Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues

Annual Review of Cell and Developmental Biology, Год журнала: 2022, Номер 38(1), С. 321 - 347

Опубликована: Май 14, 2022

Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, embryos. Mathematical frameworks have been devised to account for self-organization biological patterns, most famously Turing framework. can be defined space, example, form stripes; time, such as during oscillations; or both, traveling waves. The formation these patterns different origins: purely chemical, mechanical, a combination two. Beyond variety molecular implementations we emphasize unitary principles associated with them, across scales space within general mechanochemical We illustrate where mechanisms pattern arise from cellular tissue scales, an emphasis on morphogenesis. Our goal is convey picture that draws attention rather than solely specific mechanisms.

Язык: Английский

---

Measuring and manipulating mechanical forces during development

Nature Cell Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Язык: Английский

---

Orchestrating morphogenesis: building the body plan by cell shape changes and movements

Development, Год журнала: 2020, Номер 147(17)

Опубликована: Сен. 1, 2020

ABSTRACT During embryonic development, a simple ball of cells re-shapes itself into the elaborate body plan an animal. This requires dramatic cell shape changes and movements, powered by contractile force generated actin myosin linked to plasma membrane at cell-cell cell-matrix junctions. Here, we review three morphogenetic events common most animals: apical constriction, convergent extension collective migration. Using fruit fly Drosophila as example, discuss recent work that has revealed exciting new insights molecular mechanisms allow change move without tearing tissues apart. We also point out parallel in other animals, which suggest underlying these processes are conserved.

Язык: Английский

---

Convergent extension requires adhesion-dependent biomechanical integration of cell crawling and junction contraction

Cell Reports, Год журнала: 2022, Номер 39(4), С. 110666 - 110666

Опубликована: Апрель 1, 2022

Convergent extension (CE) is an evolutionarily conserved collective cell movement that elongates several organ systems during development. Studies have revealed two distinct cellular mechanisms, one based on crawling and the other junction contraction. Whether these behaviors collaborate unclear. Here, using live-cell imaging, we show contraction act both independently jointly but CE more effective when they are integrated via mechano-reciprocity. We thus developed a computational model considering This recapitulates biomechanical efficacy of integrating modes further clarifies how their integration influenced by adhesion. Finally, use insights to understand function understudied catenin, Arvcf, CE. These data significant for providing interesting biological into fundamental morphogenetic process implicated in human neural tube defects skeletal dysplasias.

Язык: Английский

---

Appreciating the role of cell shape changes in the mechanobiology of epithelial tissues

Biophysics Reviews, Год журнала: 2022, Номер 3(1)

Опубликована: Март 1, 2022

The wide range of epithelial cell shapes reveals the complexity and diversity intracellular mechanisms that serve to construct their morphology regulate functions. Using mechanosensitive steps, cells can sense a variety different mechanochemical stimuli adapt behavior by reshaping morphology. These changes shape rely on structural reorganization in space time generates modifications tensional state activates biochemical cascades. Recent studies have started unveil how maintenance is involved mechanical homeostatic tasks sustain tissue folding, identity, self-renewal. Here, we review relevant works integrated mechanobiology elucidate some core principles may be conveyed into spatial information guide collective processes such as morphogenesis. Among many other parameters, show regulation understood result interplay between two counteracting mechanisms: actomyosin contractility intercellular adhesions, both do not act independently but are functionally operate molecular, cellular, scales. We highlight role cadherin-based adhesions force-sensing mechanotransduction, report recent developments exploit physics liquid crystals connect orientational order aggregates. Finally, emphasize further intermingling disciplines develop new assays will lead way toward unified picture contribution pathophysiological tissues.

Язык: Английский

---

Multi-level Force-dependent Allosteric Enhancement of αE-catenin Binding to F-actin by Vinculin

Journal of Molecular Biology, Год журнала: 2023, Номер 435(5), С. 167969 - 167969

Опубликована: Янв. 20, 2023

Язык: Английский

---

Spatially coordinated cell cycle activity and motility govern bifurcation of mammary branches

The Journal of Cell Biology, Год журнала: 2023, Номер 222(9)

Опубликована: Июнь 27, 2023

Branching morphogenesis is an evolutionary solution to maximize epithelial function in a compact organ. It involves successive rounds of branch elongation and point formation generate tubular network. In all organs, points can form by tip splitting, but it unclear how cells coordinate branching. Here, we addressed these questions the embryonic mammary gland. Live imaging revealed that tips advance directional cell migration relies upon differential motility feeds retrograde flow lagging into trailing duct, supported proliferation. Tip bifurcation involved localized repression cycle at point. Cells nascent daughter remained proliferative changed their direction elongate new branches. We also report fundamental importance contractility for branching morphogenesis. The co-localization motility, non-muscle myosin II, ERK activities front suggests coordination/cooperation between functions.

Язык: Английский

---

Cyclic stretch regulates epithelial cell migration in a frequency dependent manner via intercellular vinculin recruitment

npj Biological Physics and Mechanics., Год журнала: 2024, Номер 1(1)

Опубликована: Ноя. 6, 2024

Abstract The epithelial microenvironment is incredibly dynamic, subjected to mechanical cues including cyclic stretch. While cell stretching platforms have revealed reorientation and gap formation, few studies investigated the long-term effects of stretch on migration. We measured migratory response epithelium a range physiologically relevant frequencies Our results indicate that lower (i.e., 0.1 Hz) suppress migration, accompanied by high shape solidity. found this also increased recruitment vinculin cell-cell contacts, necessary movements. These confirm mechanosensitive nature within adherens junction, but independently reveal novel mechanism low frequency stress in supporting integrity suppressing

Язык: Английский

---

An analysis of contractile and protrusive cell behaviors at the superficial surface of the zebrafish neural plate

Developmental Dynamics, Год журнала: 2025, Номер unknown

Опубликована: Фев. 22, 2025

Abstract Background The forces underlying convergence and internalization of the teleost neural plate remain unknown. To help understand this morphogenesis, we analyzed collective individual cell behaviors at superficial surface as begins to form keel in hindbrain region zebrafish embryo. Results Convergence midline is not accompanied by anteroposterior elongation stage, it characterized oscillatory contractile plate, a punctate distribution Cdh2 medially polarized actin‐rich protrusions plate. We also characterize intimate relationship dynamic protrusive between surfaces motile stationary overlying non‐neural enveloping layer. Conclusions Superficial cells are coupled Cdh2‐rich adhesions. At surface, tug on neighbors using contractions. Oscillatory contractions accompany shrinkage cells' for during keeling. Some occurs without oscillations. deep layer contact with suggesting that may constrain movements internalization.

Язык: Английский

---

Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation

Опубликована: Март 6, 2025

Apical constriction is a basic mechanism for epithelial morphogenesis, making columnar cells into wedge shape and bending flat cell sheet. It has long been thought that an apically localized myosin generates contractile force drives the deformation. However, when we tested increased apical surface contractility in cellular Potts model simulation, pressure inside pushed its lateral outward, adopt drop instead of expected shape. To keep straight, considered alternative which was determined by membrane elasticity endocytosis, balanced among cells. The simulation succeeded reproducing constriction, it also suggested too strong tension might prevent tissue invagination.

Язык: Английский

---