Investigating the basis of lineage decisions and developmental trajectories in the dorsal spinal cord through pseudotime analyses DOI Creative Commons
Sandeep Gupta, Eric Heinrichs, Bennett G. Novitch

и другие.

Development, Год журнала: 2024, Номер 151(10)

Опубликована: Май 15, 2024

ABSTRACT Dorsal interneurons (dIs) in the spinal cord encode perception of touch, pain, heat, itchiness and proprioception. Previous studies using genetic strategies animal models have revealed important insights into dI development, but molecular details how dIs arise as distinct populations neurons remain incomplete. We developed a resource to investigate fate specification by combining single-cell RNA-Seq atlas mouse embryonic stem cell-derived with pseudotime analyses. To validate this silico useful tool, we used it first identify genes that are candidates for directing transition states lead lineage trajectories, then validated them situ hybridization analyses developing vivo. also identified an endpoint dI5 trajectory found become more transcriptionally homogeneous during terminal differentiation. This study introduces valuable tool further discovery about timing gene expression differentiation demonstrates its utility clarifying relationships.

Язык: Английский

An active metabolite of the anti-COVID-19 drug molnupiravir impairs mouse preimplantation embryos at clinically relevant concentrations DOI Creative Commons
Yusuke Marikawa, Vernadeth B. Alarcón

Reproductive Toxicology, Год журнала: 2023, Номер 121, С. 108475 - 108475

Опубликована: Сен. 23, 2023

Язык: Английский

Процитировано

5
Wnt and BMP signalling direct anterior–posterior differentiation in aggregates of mouse embryonic stem cells DOI Creative Commons
Atoosa Amel, Alexa Rabeling,

Simoné Rossouw

и другие.

Biology Open, Год журнала: 2023, Номер 12(9)

Опубликована: Авг. 25, 2023

ABSTRACT Stem-cell-based embryo models have allowed greater insight into peri-implantation mammalian developmental events that are otherwise difficult to manipulate due the inaccessibility of early embryo. The rapid development this field has resulted in precise roles frequently used supplements such as N2, B27 and Chiron driving stem cell lineage commitment not being clearly defined. Here, we investigate effects these on embryoid bodies better understand their differentiation. We show Wnt signalling a general posteriorising effect aggregates directs differentiation towards mesoderm, confirmed through upregulation posterior mesodermal markers. N2 can mitigate upregulate expression anterior To control gradient subsequent versus fate, make use BMP4 centre conditions express cephalic These findings indicate there is an intricate balance between various culture ability guide models.

Язык: Английский

Процитировано

4
Mapping and exploring the organoid state space using synthetic biology DOI
Tzer Han Tan, Jifeng Liu, Anne Grapin‐Botton

и другие.

Seminars in Cell and Developmental Biology, Год журнала: 2022, Номер 141, С. 23 - 32

Опубликована: Апрель 22, 2022

Язык: Английский

Процитировано

7
A stem cell zoo uncovers intracellular scaling of developmental tempo across mammals DOI Creative Commons
Jorge Lázaro, Maria Costanzo, Marina Sanaki-Matsumiya

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Окт. 14, 2022

Abstract Differential speeds in biochemical reactions have been proposed to be responsible for the differences developmental tempo between mice and humans. However, underlying mechanism controlling species-specific kinetics remains determined. Using vitro differentiation of pluripotent stem cells, we recapitulated segmentation clocks diverse mammalian species varying body weight taxa: marmoset, rabbit, cattle rhinoceros. Together with mouse human, clock periods six did not scale animal weight, but were rather grouped according phylogeny. The core gene HES7 displayed clear scaling period. cellular metabolic rates show an evident correlation. Instead, genes involving showed expression pattern that scales Altogether, our cell zoo uncovered general laws governing tempo.*

Язык: Английский

Процитировано

7
Investigating the basis of lineage decisions and developmental trajectories in the dorsal spinal cord through pseudotime analyses DOI Creative Commons
Sandeep Gupta, Eric Heinrichs, Bennett G. Novitch

и другие.

Development, Год журнала: 2024, Номер 151(10)

Опубликована: Май 15, 2024

ABSTRACT Dorsal interneurons (dIs) in the spinal cord encode perception of touch, pain, heat, itchiness and proprioception. Previous studies using genetic strategies animal models have revealed important insights into dI development, but molecular details how dIs arise as distinct populations neurons remain incomplete. We developed a resource to investigate fate specification by combining single-cell RNA-Seq atlas mouse embryonic stem cell-derived with pseudotime analyses. To validate this silico useful tool, we used it first identify genes that are candidates for directing transition states lead lineage trajectories, then validated them situ hybridization analyses developing vivo. also identified an endpoint dI5 trajectory found become more transcriptionally homogeneous during terminal differentiation. This study introduces valuable tool further discovery about timing gene expression differentiation demonstrates its utility clarifying relationships.

Язык: Английский

Процитировано

1