ABSTRACT
Stem-cell-based
embryo
models
have
allowed
greater
insight
into
peri-implantation
mammalian
developmental
events
that
are
otherwise
difficult
to
manipulate
due
the
inaccessibility
of
early
embryo.
The
rapid
development
this
field
has
resulted
in
precise
roles
frequently
used
supplements
such
as
N2,
B27
and
Chiron
driving
stem
cell
lineage
commitment
not
being
clearly
defined.
Here,
we
investigate
effects
these
on
embryoid
bodies
better
understand
their
differentiation.
We
show
Wnt
signalling
a
general
posteriorising
effect
aggregates
directs
differentiation
towards
mesoderm,
confirmed
through
upregulation
posterior
mesodermal
markers.
N2
can
mitigate
upregulate
expression
anterior
To
control
gradient
subsequent
versus
fate,
make
use
BMP4
centre
conditions
express
cephalic
These
findings
indicate
there
is
an
intricate
balance
between
various
culture
ability
guide
models.
Communications Biology,
Год журнала:
2023,
Номер
6(1)
Опубликована: Апрель 28, 2023
Abstract
Biomechanical
cues
from
the
extracellular
matrix
(ECM)
are
essential
for
directing
many
cellular
processes,
normal
development
and
repair,
to
disease
progression.
To
better
understand
cell-matrix
interactions,
we
have
developed
a
new
instrument
named
‘OptoRheo’
that
combines
light
sheet
fluorescence
microscopy
with
particle
tracking
microrheology.
OptoRheo
lets
us
image
cells
in
3D
as
they
proliferate
over
several
days
while
simultaneously
sensing
mechanical
properties
of
surrounding
pericellular
at
sub-cellular
length
scale.
can
be
used
two
operational
modalities
(with
without
an
optical
trap)
extend
dynamic
range
microrheology
measurements.
We
corroborated
this
by
characterising
ECM
live
breast
cancer
distinct
culture
systems,
cell
clusters
hydrogels
spheroids
suspension
culture.
This
cutting-edge
will
transform
exploration
drug
transport
through
complex
matrices
optimise
design
next-generation
models.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Окт. 14, 2022
Abstract
Differential
speeds
in
biochemical
reactions
have
been
proposed
to
be
responsible
for
the
differences
developmental
tempo
between
mice
and
humans.
However,
underlying
mechanism
controlling
species-specific
kinetics
remains
determined.
Using
vitro
differentiation
of
pluripotent
stem
cells,
we
recapitulated
segmentation
clocks
diverse
mammalian
species
varying
body
weight
taxa:
marmoset,
rabbit,
cattle
rhinoceros.
Together
with
mouse
human,
clock
periods
six
did
not
scale
animal
weight,
but
were
rather
grouped
according
phylogeny.
The
core
gene
HES7
displayed
clear
scaling
period.
cellular
metabolic
rates
show
an
evident
correlation.
Instead,
genes
involving
showed
expression
pattern
that
scales
Altogether,
our
cell
zoo
uncovered
general
laws
governing
tempo.*
ABSTRACT
Stem-cell-based
embryo
models
have
allowed
greater
insight
into
peri-implantation
mammalian
developmental
events
that
are
otherwise
difficult
to
manipulate
due
the
inaccessibility
of
early
embryo.
The
rapid
development
this
field
has
resulted
in
precise
roles
frequently
used
supplements
such
as
N2,
B27
and
Chiron
driving
stem
cell
lineage
commitment
not
being
clearly
defined.
Here,
we
investigate
effects
these
on
embryoid
bodies
better
understand
their
differentiation.
We
show
Wnt
signalling
a
general
posteriorising
effect
aggregates
directs
differentiation
towards
mesoderm,
confirmed
through
upregulation
posterior
mesodermal
markers.
N2
can
mitigate
upregulate
expression
anterior
To
control
gradient
subsequent
versus
fate,
make
use
BMP4
centre
conditions
express
cephalic
These
findings
indicate
there
is
an
intricate
balance
between
various
culture
ability
guide
models.
