Single-cell transcriptomics identifies regulation of invasive behavior in Drosophila follicle cells with polarity loss DOI Creative Commons
Deeptiman Chatterjee, Xianfeng Wang, Allison Jevitt

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Июнь 12, 2022

ABSTRACT Apicobasal cell-polarity loss is a founding event in Epithelial-Mesenchymal Transition (EMT) and epithelial tumorigenesis, yet how pathological polarity induces plasticity changes remains largely unknown. To understand the mechanisms mediators regulating upon loss, we performed single-cell (sc) RNA sequencing of Drosophila ovaries, where inducing polarity-gene l(2)gl knockdown (Lgl-KD) causes invasive delamination follicular epithelia. Integrating Lgl-KD with corresponding wild-type sc-transcriptome, discovered clusters specific to various discernible phenotype-specific cell types further characterized regulons active those cells. A genetic requirement Keap1-Nrf2 signaling promoting multilayer formation cells was identified. Elevated expression Keap1 increased volume delaminated follicle that undergo enhanced collective invasion via cytoskeletal remodeling. Overall, our findings describe comprehensive transcriptome follicle-cell tumor model at resolution identify previously unappreciated link between stress early tumorigenesis.

Язык: Английский

Single-cell transcriptomics identifies regulation of invasive behavior in Drosophila follicle cells with polarity loss DOI Creative Commons
Deeptiman Chatterjee, Xianfeng Wang, Allison Jevitt

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Июнь 12, 2022

ABSTRACT Apicobasal cell-polarity loss is a founding event in Epithelial-Mesenchymal Transition (EMT) and epithelial tumorigenesis, yet how pathological polarity induces plasticity changes remains largely unknown. To understand the mechanisms mediators regulating upon loss, we performed single-cell (sc) RNA sequencing of Drosophila ovaries, where inducing polarity-gene l(2)gl knockdown (Lgl-KD) causes invasive delamination follicular epithelia. Integrating Lgl-KD with corresponding wild-type sc-transcriptome, discovered clusters specific to various discernible phenotype-specific cell types further characterized regulons active those cells. A genetic requirement Keap1-Nrf2 signaling promoting multilayer formation cells was identified. Elevated expression Keap1 increased volume delaminated follicle that undergo enhanced collective invasion via cytoskeletal remodeling. Overall, our findings describe comprehensive transcriptome follicle-cell tumor model at resolution identify previously unappreciated link between stress early tumorigenesis.

Язык: Английский

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