In Vitro Models of Cardiovascular Disease: Embryoid Bodies, Organoids and Everything in Between DOI Creative Commons
Theodora M. Stougiannou, Konstantinos Christodoulou, Dimos Karangelis

и другие.

Biomedicines, Год журнала: 2024, Номер 12(12), С. 2714 - 2714

Опубликована: Ноя. 27, 2024

Cardiovascular disease comprises a group of disorders affecting or originating within tissues and organs the cardiovascular system; most, if not all, will eventually result in cardiomyocyte dysfunction death, negatively impacting cardiac function. Effective models are thus important for understanding crucial aspects progression, while recent advancements stem cell biology have allowed use populations to derive such models. These include three-dimensional (3D) as cell-based embryos (SCME) well organoids, many which frequently derived from embryoid bodies (EB). Not only can they recapitulate 3D form function, but developmental programs governing self-organization into more complex well. Many different organoids SCME constructs been generated years recreate tissue that give rise its cellular composition unique morphology. It is purpose this narrative literature review describe summarize recently organoid their recapitulation genetic acquired disease. Owing examined, focus on injury associated with embryonic/fetal tissues.

Язык: Английский

Brachyury co-operates with polycomb protein RYBP to regulate gastrulation and axial elongation in vitro DOI Creative Commons

Lilla Kokity,

Zsolt Czimmerer,

Bernadett Benyhe-Kis

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Ноя. 29, 2024

Early embryonic development is a complex process where undifferentiated cells lose their pluripotency and start to gastrulate. During gastrulation, three germ layers form, giving rise different cell lineages organs. This regulated by transcription factors epigenetic regulators, including non-canonical polycomb repressive 1s (ncPRC1s). Previously, we reported that ncPRC1-member RYBP (RING1 YY1 binding protein) crucial for implantation cardiac lineage commitment in mice. However, the role of gastrulation mesoderm formation has not yet been defined. In this study, used 2D 3D

Язык: Английский

Процитировано

1

EomesrestrictsBrachyuryfunctions at the onset of mammalian gastrulation DOI Creative Commons
Katrin M. Schüle, Jelena Weckerle, Simone Probst

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Янв. 27, 2023

Abstract Mammalian specification of mesoderm and definitive endoderm (DE) is instructed by the two related Tbx transcription factors (TFs) Eomesodermin ( Eomes ) Brachyury sharing partially redundant functions. Gross differences mutant embryonic phenotypes suggest specific functions each TF. To date, molecular details separated lineage-specific gene-regulation remain poorly understood. Here, we combine stem cell-based analyses to delineate non-overlapping, transcriptional activities. On a genome-wide scale binding both TFs overlaps at promoters target genes, but shows specificity for distal enhancer regions, that conferred in DNA-binding motifs. The unique sites instructs anterior (AM) DE caudal . Remarkably, EOMES antagonizes BRACHYURY gene-regulatory co-expressing cells during early gastrulation ensure proper sequence AM lineage followed posterior derivatives. Highlights Detailed comparative analysis critical developmental regulators mouse embryos differentiating control distinct gene programs specify different subsets Program non-overlapping enhancers with motifs restricts activities thus ensuring

Язык: Английский

Процитировано

3

Local cellular interactions during the self-organization of stem cells DOI Open Access
Christian Schröter, Kristina S. Stapornwongkul, Vikas Trivedi

и другие.

Current Opinion in Cell Biology, Год журнала: 2023, Номер 85, С. 102261 - 102261

Опубликована: Окт. 28, 2023

Язык: Английский

Процитировано

1

BPA Exposure Affects Mouse Gastruloids Axial Elongation by Perturbing the Wnt/β-Catenin Pathway DOI Open Access
Paola Rebuzzini,

S Rustichelli,

Lorenzo Fassina

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7924 - 7924

Опубликована: Июль 19, 2024

Mammalian embryos are very vulnerable to environmental toxicants (ETs) exposure. Bisphenol A (BPA), one of the most diffused ETs, exerts endocrine-disrupting effects through estro-gen-mimicking and hormone-like properties, with detrimental health effects, including on reproduction. However, its impact during peri-implantation stages is still unclear. This study, using gastruloids as a 3D stem cell-based in vitro model embryonic development, showed that BPA exposure arrests their axial elongation when present Wnt/β-catenin pathway activation period by β-catenin protein reduction. Gastruloid reshaping might have been impeded downregulation Snail, Slug Twist, known suppress E-cadherin expression activate N-cadherin gene, low protein. Also, lack be related altered exit BPA-exposed cells from pluripotency condition following differentiation. In conclusion, here we show inhibition gastruloids' result concomitant perturbation, reduced Oct4, T/Bra Cdx2 patter expression, which all together concur impaired development mouse gastruloids.

Язык: Английский

Процитировано

0

In Vitro Models of Cardiovascular Disease: Embryoid Bodies, Organoids and Everything in Between DOI Creative Commons
Theodora M. Stougiannou, Konstantinos Christodoulou, Dimos Karangelis

и другие.

Biomedicines, Год журнала: 2024, Номер 12(12), С. 2714 - 2714

Опубликована: Ноя. 27, 2024

Cardiovascular disease comprises a group of disorders affecting or originating within tissues and organs the cardiovascular system; most, if not all, will eventually result in cardiomyocyte dysfunction death, negatively impacting cardiac function. Effective models are thus important for understanding crucial aspects progression, while recent advancements stem cell biology have allowed use populations to derive such models. These include three-dimensional (3D) as cell-based embryos (SCME) well organoids, many which frequently derived from embryoid bodies (EB). Not only can they recapitulate 3D form function, but developmental programs governing self-organization into more complex well. Many different organoids SCME constructs been generated years recreate tissue that give rise its cellular composition unique morphology. It is purpose this narrative literature review describe summarize recently organoid their recapitulation genetic acquired disease. Owing examined, focus on injury associated with embryonic/fetal tissues.

Язык: Английский

Процитировано

0