Planar cell polarity is essential for the architectural patterning of the mammalian biliary tree DOI Creative Commons
Michaela Raab, Ersi Christodoulou, Roopesh Krishnankutty

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 20, 2023

Abstract In the developing liver, bipotent epithelial progenitor cells known as hepatoblasts undergo lineage segregation to form two major cell types, hepatocytes that constitute bulk of liver parenchyma and biliary (cholangiocytes) which comprise bile duct, a complex tubular network is critical for normal function. Notch TGFβ signalling promote formation sheet cells, ductal plate organises into discontinuous structures. How these structures elongate connect continuous duct remains undefined. Here, we show planar polarity protein, VANGL2 expressed late in intrahepatic development patterns cell-cell contacts between cells. The patterning regulates polarisation actin cytoskeleton within loss Vangl2 -function results abnormal distribution cortical remodelling resulting failure formation. Planar step post-specification sculpture essential establishing tissue architecture.

Язык: Английский

Van Gogh-like 2 is essential for the architectural patterning of the mammalian biliary tree DOI Creative Commons
Michaela Raab, Ersi Christodoulou, Roopesh Krishnankutty

и другие.

Journal of Hepatology, Год журнала: 2024, Номер 81(1), С. 108 - 119

Опубликована: Март 7, 2024

In the developing liver, bipotent epithelial progenitor cells undergo lineage segregation to form hepatocytes, which constitute bulk of liver parenchyma, and biliary (cholangiocytes), comprise bile duct (a complex tubular network that is critical for normal function). Notch TGFβ signalling promote formation a sheet cells, ductal plate, organises into discontinuous structures. How these structures elongate connect continuous remains undefined. We aimed define mechanisms by plate transitions from simple connected duct.

Язык: Английский

Процитировано

2
Emergence of planar cell polarity from the interplay of local interactions and global gradients DOI Creative Commons
Divyoj Singh, Sriraṁ Ramaswamy, Mohit Kumar Jolly

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Окт. 25, 2024

Planar cell polarity (PCP) - tissue-scale alignment of the direction asymmetric localization proteins at cell-cell interface is essential for embryonic development and physiological functions. Abnormalities in PCP can result developmental imperfections, including neural tube closure defects misaligned hair follicles. Decoding mechanisms responsible establishment maintenance remains a fundamental open question. While roles various molecules broadly classified into 'global' 'local' modules have been well-studied, their necessity sufficiency explaining connecting perturbations to experimentally observed patterns not examined. Here, we develop minimal model that captures proposed features global tissue-level gradient local distribution protein complexes. The suggests while emerge without gradient, only acts as cue but also increases robustness against stochastic perturbations. We recapitulated quantified swirling domineering non-autonomy, using three free parameters rate binding membrane, concentration proteins, steepness. explain how self-stabilizing localizations presence lead robust reveal design principles polarized system.

Язык: Английский

Процитировано

0
Planar cell polarity is essential for the architectural patterning of the mammalian biliary tree DOI Creative Commons
Michaela Raab, Ersi Christodoulou, Roopesh Krishnankutty

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 20, 2023

Abstract In the developing liver, bipotent epithelial progenitor cells known as hepatoblasts undergo lineage segregation to form two major cell types, hepatocytes that constitute bulk of liver parenchyma and biliary (cholangiocytes) which comprise bile duct, a complex tubular network is critical for normal function. Notch TGFβ signalling promote formation sheet cells, ductal plate organises into discontinuous structures. How these structures elongate connect continuous duct remains undefined. Here, we show planar polarity protein, VANGL2 expressed late in intrahepatic development patterns cell-cell contacts between cells. The patterning regulates polarisation actin cytoskeleton within loss Vangl2 -function results abnormal distribution cortical remodelling resulting failure formation. Planar step post-specification sculpture essential establishing tissue architecture.

Язык: Английский

Процитировано

0