T cell receptor–centric perspective to multimodal single-cell data analysis DOI Creative Commons
Kerry A. Mullan, My Kieu Ha, Sebastiaan Valkiers

и другие.

Science Advances, Год журнала: 2024, Номер 10(48)

Опубликована: Ноя. 29, 2024

The T cell receptor (TCR), despite its importance, is underutilized in single-cell analysis, with gene expression features solely driving current strategies. Here, we argue for a TCR-first approach, more suited toward repertoires. To this end, curated large atlas from 12 prominent human studies, containing total 500,000 cells spanning multiple diseases, including melanoma, head and neck cancer, blood lung transplantation. identified severe limitations cell-type annotation using unsupervised approaches propose robust standard semi-supervised method or the TCR arrangement. We showcase utility of approach through application STEGO.R tool identification treatment-related dynamics previously unknown public clusters potential antigen-specific properties. Thus, paradigm shift to can highlight overlooked key that have improvements immunotherapy diagnostics.

Язык: Английский

T cell receptor–centric perspective to multimodal single-cell data analysis DOI Creative Commons
Kerry A. Mullan, My Kieu Ha, Sebastiaan Valkiers

и другие.

Science Advances, Год журнала: 2024, Номер 10(48)

Опубликована: Ноя. 29, 2024

The T cell receptor (TCR), despite its importance, is underutilized in single-cell analysis, with gene expression features solely driving current strategies. Here, we argue for a TCR-first approach, more suited toward repertoires. To this end, curated large atlas from 12 prominent human studies, containing total 500,000 cells spanning multiple diseases, including melanoma, head and neck cancer, blood lung transplantation. identified severe limitations cell-type annotation using unsupervised approaches propose robust standard semi-supervised method or the TCR arrangement. We showcase utility of approach through application STEGO.R tool identification treatment-related dynamics previously unknown public clusters potential antigen-specific properties. Thus, paradigm shift to can highlight overlooked key that have improvements immunotherapy diagnostics.

Язык: Английский

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