Impact of Metal Ions on Cellular Functions: A Focus on Mesenchymal Stem/Stromal Cell Differentiation
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 10127 - 10127
Опубликована: Сен. 20, 2024
Metals
play
a
crucial
role
in
the
human
body,
especially
as
ions
metalloproteins.
Essential
metals,
such
calcium,
iron,
and
zinc
are
for
various
physiological
functions,
but
their
interactions
within
biological
networks
complex
not
fully
understood.
Mesenchymal
stem/stromal
cells
(MSCs)
essential
tissue
regeneration
due
to
ability
differentiate
into
cell
types.
This
review
article
addresses
effects
of
unphysiological,
directly
toxic,
metal
ion
concentrations,
particularly
concerning
MSCs.
Overloading
or
unbalancing
concentrations
can
significantly
impair
function
differentiation
capacity
In
addition,
excessive
unbalanced
lead
oxidative
stress,
which
affect
viability
inflammation.
Data
on
MSC
limited
often
contradictory.
Future
research
should,
therefore,
aim
clarify
mechanisms
by
differentiation,
focusing
aspects
interactions,
exposure
duration,
other
environmental
conditions.
Understanding
these
could
ultimately
improve
design
biomaterials
implants
promote
MSC-mediated
regeneration.
It
also
development
innovative
therapeutic
strategies
regenerative
medicine.
Язык: Английский
A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth
Nature Structural & Molecular Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 24, 2025
Abstract
Cilia
are
motile
or
sensory
organelles
present
on
many
eukaryotic
cells.
Their
formation
and
function
rely
axonemal
microtubules,
which
exhibit
very
slow
dynamics,
but
the
underlying
mechanisms
largely
unexplored.
Here
we
reconstituted
in
vitro
individual
collective
activities
of
ciliary
tip
module
proteins
CEP104,
CSPP1,
TOGARAM1,
ARMC9
CCDC66,
interact
with
each
other
microtubules
and,
when
mutated
humans,
cause
ciliopathies
such
as
Joubert
syndrome.
We
show
that
a
protein
tubulin-binding
TOG
domain,
its
luminal
partner
CSPP1
inhibit
microtubule
growth
shortening.
Another
TOG-domain
protein,
overcomes
inhibition
imposed
by
CEP104
CSPP1.
CCDC66
do
not
affect
dynamics
act
scaffolds
for
their
partners.
Cryo-electron
tomography
demonstrated
that,
together,
members
form
plus-end-specific
cork-like
structures
reduce
protofilament
flaring.
The
combined
effect
these
is
processive
elongation,
recapitulates
Язык: Английский
Axonemal microtubule dynamics in the assembly and disassembly of cilia
Biochemical Society Transactions,
Год журнала:
2025,
Номер
53(1)
Опубликована: Янв. 31, 2025
Cilia
and
eukaryotic
flagella
(exchangeable
terms)
function
in
cell
motility
signaling,
which
are
pivotal
for
development
physiology.
dysfunction
can
lead
to
ciliopathies.
usually
assembled
quiescent
and/or
differentiated
cells
undergo
disassembly
when
enter
cycle
or
response
environmental
stresses.
contain
a
microtubule-based
structure
termed
axoneme
that
comprises
nine
outer
doublet
microtubules
with
without
pair
of
central
microtubules,
is
ensheathed
by
the
ciliary
membrane.
Regulation
axonemal
microtubule
dynamics
tightly
associated
assembly
disassembly.
In
this
short
review,
we
discuss
recent
findings
on
regulation
microtubule-binding
proteins
modulating
kinesins
during
Язык: Английский
Centrosome biogenesis and maintenance in homeostasis and disease
Current Opinion in Cell Biology,
Год журнала:
2025,
Номер
94, С. 102485 - 102485
Опубликована: Фев. 24, 2025
Язык: Английский
Phosphorylation at the Helm: Kinase‐Mediated Regulation of Primary Cilia Assembly and Disassembly
Cytoskeleton,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 10, 2025
The
primary
cilium
serves
as
an
antenna
of
most
vertebrate
cells
and
is
important
for
conveying
cues
from
several
signaling
pathways
into
appropriate
cellular
responses
during
development
homeostasis.
Cilia
assembly
disassembly
processes
are
thought
to
be
strictly
controlled;
however,
the
precise
nature
molecular
events
underlying
this
control
still
awaits
full
resolution.
Through
their
enzymatic
activity,
kinases
function
flexible
yet
highly
controllable
regulators
a
vast
variety
processes.
Their
activity
ranges
cell
cycle
regulation
motility,
signal
transduction,
metabolism.
This
review
focuses
on
emerging
role
in
cilia
biology.
We
underscore
functions
formation,
maintenance,
resorption
while
examining
available
models
respective
mechanisms
actions.
Язык: Английский
Microtubule-Associated Proteins (MAPs) Are Multifunctional Cytoskeletal Proteins in the Testis That Regulate Spermatogenesis
Advances in experimental medicine and biology,
Год журнала:
2025,
Номер
unknown, С. 411 - 431
Опубликована: Янв. 1, 2025
Язык: Английский
A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 26, 2024
Abstract
Cilia
are
essential
motile
or
sensory
organelles
found
on
many
eukaryotic
cells.
Their
formation
and
function
rely
axonemal
microtubules,
which
exhibit
very
slow
dynamics,
however
the
underlying
biochemical
mechanisms
largely
unexplored.
Here,
we
reconstituted
in
vitro
individual
collective
activities
of
ciliary
tip
module
proteins,
CEP104,
CSPP1,
TOGARAM1,
ARMC9
CCDC66,
interact
with
each
other
and,
when
mutated,
cause
ciliopathies
such
as
Joubert
syndrome.
a
protein
containing
tubulin-binding
TOG
domain,
is
an
inhibitor
microtubule
growth
shortening
that
interacts
EBs
surface
luminal
microtubule-pausing
factor
CSPP1.
Another
TOG-domain
protein,
overcomes
inhibition
imposed
by
CEP104
CCDC66
do
not
affect
dynamics
directly
but
act
scaffolds
for
their
partners.
Cryo-electron
tomography
showed
together,
members
form
plus-end-specific
cork-like
structures
reduce
protofilament
flaring.
The
combined
effect
these
proteins
processive
elongation,
recapitulates
Язык: Английский
Variants in the AGBL5 gene are responsible for autosomal recessive Retinitis pigmentosa with hearing loss
European Journal of Human Genetics,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 13, 2024
Abstract
The
AGBL5
gene
encodes
for
the
Cytoplasmic
Carboxypeptidase
5
(CCP5),
an
α-tubulin
deglutamylase
that
cleaves
γ-carboxyl-linked
branching
point
of
glutamylated
tubulin.
To
date,
pathogenic
variants
in
have
been
associated
only
with
isolated
retinitis
pigmentosa
(RP).
Hearing
loss
has
not
reported
-caused
retinal
disease.
In
this
study,
we
performed
exome
sequencing
probands
eight
unrelated
families
from
Italy,
Spain,
Palestine,
Switzerland,
and
Greece.
All
subjects
had
a
clinical
diagnosis
(suspected)
Usher
syndrome
type
II
concurrent
presence
RP
post-verbal
sensorineural
hearing
(SNHL)
ranged
mild
to
moderate.We
identified
biallelic
sequence
all
analysed
subjects.
Four
were
novel.
co-segregated
auditory
phenotypes
additional
affected
family
members.
We
did
detect
any
causative
known
deafness
or
genes
could
explain
patients’
loss.
therefore
conclude
SNHL
is
feature
syndromic
presentation
retinopathy.
This
study
provides
first
evidence
mutations
can
cause
forms
loss,
probably
due
dysfunction
sensory
cilia
retina
inner
ear.
Язык: Английский
Ccdc66 regulates primary cilium stability, disassembly and signaling important for epithelial organization
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 17, 2024
Abstract
The
primary
cilium
is
a
conserved,
microtubule-based
organelle
that
transduces
signaling
pathways
essential
for
development
and
homeostasis.
It
dynamic
structure
assembles
disassembles
in
response
to
intrinsic
extrinsic
stimuli
while
maintaining
remarkable
stability
tightly
controlled
length.
Although
assembly
well-understood,
less
known
about
the
molecular
players
governing
their
stability,
length
disassembly.
Here,
we
elucidated
function
of
Ccdc66,
microtubule-associated
protein
linked
ciliopathies,
maintenance
disassembly
mouse
epithelial
cells.
We
found
Ccdc66
depletion
disrupts
disassembly,
but
does
not
affect
these
Live
imaging
processes
revealed
cilia
Ccdc66-depleted
cells
frequently
fluctuate
exhibit
increased
ectocytosis
from
tip.
Phenotypic
rescue
experiments
vitro
microtubule
stabilization
assays
showed
mediates
functions
via
regulating
microtubules.
Temporal
proximity
mapping
CCDC66
identified
potential
new
regulators
involved
Additionally,
compromised
Hedgehog
Wnt
pathway
activation
disrupted
cell
organization
polarity
two-dimensional
three-dimensional
cultures.
Collectively,
our
results
define
as
microtubule-stabilizing
factor
regulates
providing
insights
into
mechanisms
homeostasis
pathologies
associated
with
Ccdc66.
Язык: Английский
Motile Cilia in Female and Male Reproductive Tracts and Fertility
Cells,
Год журнала:
2024,
Номер
13(23), С. 1974 - 1974
Опубликована: Ноя. 28, 2024
Motile
cilia
are
evolutionarily
conserved
organelles.
In
humans,
multiciliated
cells
(MCCs),
assembling
several
hundred
motile
on
their
apical
surface,
components
of
the
monolayer
epithelia
lining
lower
and
upper
airways,
brain
ventricles,
parts
reproductive
tracts,
fallopian
tube
uterus
in
females,
efferent
ductules
males.
The
coordinated
beating
generates
a
force
that
enables
shift
tubular
fluid,
particles,
or
along
surface
ciliated
epithelia.
Uncoordinated
altered
motion
immotility
may
result
subfertility
even
infertility.
Here,
we
summarize
current
knowledge
regarding
localization
function
MCCs
human
discuss
how
beating-generated
fluid
flow
directly
indirectly
contribute
to
processes
these
organs,
lack
improper
functioning
influence
fertility.
Язык: Английский