Luciferase complementation for cellular assays beyond protein–protein interactions

Analytical Sciences, Год журнала: 2025, Номер unknown

Опубликована: Фев. 18, 2025

Abstract Luciferase complementation assays have emerged in 2001 as a useful tool to analyze biological processes through diverse such cellular studies and vivo imaging. The assay has an advantage of wide dynamic ranges, high signal-to-noise ratios, capability for real-time monitoring events with readout bioluminescence. While it was initially harnessed detecting protein–protein interactions, biosensors based on luciferase-fragment achieved significant advancements their designs, expanding versatility applicability beyond the initial scope. This review aims provide comprehensive overview designing strategies employed split luciferase highlight bioanalytical applications. Because simple bi-molecular detection interactions by this approach is well-established, will focus introducing sensor designs using concept complementation. Graphical abstract

Язык: Английский

Research approaches for exploring the hidden conversations of GPCR Transactivation

Molecular Pharmacology, Год журнала: 2025, Номер unknown, С. 100043 - 100043

Опубликована: Май 1, 2025

Язык: Английский