To hatch and hatch not: does heterochrony in onset of vestibular mechanosensing explain species differences in escape-hatching success of Agalychnis embryos in snake attacks? DOI Creative Commons
Brandon A. Güell, Karen M. Warkentin

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Авг. 10, 2023

Abstract Phyllomedusid treefrogs hatch prematurely to escape egg predation, but success varies among species. Snake attacks elicited 55% less escape-hatching in spontaneously hatching Agalychnis spurrelli than developed A. callidryas . use their vestibular system and, secondarily, lateral line sense physical disturbances that cue hatching. Since develop faster, we hypothesized heterochronic shifts the onset timing of mechanosensory function, relative ability, might explain lower response cues. To test this, compared timings hypoxia- and mechanosensory-cued (MCH) function developmental series both Across species, most sibships began responding each at same stage development, measured by vestibulo-ocular reflex (VOR), was similar. These results demonstrate low is not caused a delay function. MCH associated with VOR, higher threshold callidryas. The absence before strong suggests multimodal mechanosensing may contribute antipredator responses embryos. Our observations larger clutches snake complications flooded suggest differences clutch capsule structure directly species success. Moreover, select against false alarms, increasing stringency decision rules.

Язык: Английский

Functional Morphology of Hatching: Ontogeny and Distribution of Hatching Gland Cells in Red‐Eyed Treefrogs and a New Marker for Anuran Hatching Enzyme DOI Open Access
María José Salazar‐Nicholls,

Henry Macías Bazante,

Karen M. Warkentin

и другие.

Journal of Morphology, Год журнала: 2025, Номер 286(2)

Опубликована: Янв. 28, 2025

ABSTRACT Environmentally cued hatching (ECH) is widespread in animals and requires regulation of mechanisms. Enzymatic digestion the egg membrane a common mechanism vertebrates invertebrates. In amphibians fishes, enzymes (HE) are synthesized released by gland cells (HGC), whose functional ontogeny determines when can occur. Ontogenetic studies HGC development or HE expression limited, based largely on external cell morphology; few markers for available, those appear specific Xenopus . Moreover, mechanisms regulating release unknown anurans. To investigate variation process, we need tools to identify analyze its components. Agalychnis callidryas (Hylidae) well‐established model ECH, showing plastically timed, acute release, unlike gradual described some aquatic We developed new antibody marker A. that also labels HGC/HE glassfrogs (Centrolenidae). As treefrogs diverged 62 mya, may be broadly useful used AcHE examine distribution accumulation HE, two key elements mechanisms, found much larger number (ca. 4200) broader than has been documented any amphibian, with densely but non‐contiguously distributed over front head eyes scattered along dorsal midline. begins before competence strong throughout plastic period, gene which diminishes after competence. The callidryas' HE/HGC related their performance, plasticity, embryo morphology. will enable comparative research elucidate co‐variation morphology, ecological context hatching.

Язык: Английский

Процитировано

0
Developmental changes in red-eyed treefrog embryo behavior increase escape-hatching success in wasp attacks DOI
Elena Katherine Gomez,

Alina Chaiyasarikul,

Brandon A. Güell

и другие.

Behavioral Ecology and Sociobiology, Год журнала: 2023, Номер 77(5)

Опубликована: Май 1, 2023

Язык: Английский

Процитировано

4
Elevated ammonia cues hatching in red‐eyed treefrogs: A mechanism for escape from drying eggs DOI
Astrid K. Lisondro‐Arosemena, María José Salazar‐Nicholls, Karen M. Warkentin

и другие.

Journal of Experimental Zoology Part B Molecular and Developmental Evolution, Год журнала: 2024, Номер 342(5), С. 406 - 411

Опубликована: Май 6, 2024

Egg dehydration can kill terrestrial frog embryos, and this threat is increasing with climate change deforestation. In several lineages that independently evolved eggs, retained aquatic tadpoles, embryos accelerate hatching to escape from drying entering the water earlier less developed. However, cues stimulate drying-induced early are unknown. Ammonia a toxic, water-soluble metabolic waste accumulates within eggs as develop concentrates dehydrate. Thus, ammonia concentration may be direct in eggs. We hypothesized it could serve cue, stimulating hatch escape. The of red-eyed treefrogs, Agalychnis callidryas, many threats, including dehydration, known use mechanosensory, hypoxia, light cues. To test if they also high cue hatch, we exposed stage-matched pairs hatching-competent, well-hydrated sibling control solutions shallow baths recorded their behavior. Control remained unhatched while ammonia-exposed showed rapid, strong response; 95% hatched, on average under 15 min. This demonstrates elevated for A. callidryas embryos. finding key step understanding mechanisms enable egg drying, opening new possibilities integrative comparative studies growing threat.

Язык: Английский

Процитировано

1
To hatch and hatch not: does heterochrony in onset of vestibular mechanosensing explain species differences in escape-hatching success of Agalychnis embryos in snake attacks? DOI
Brandon A. Güell, Karen M. Warkentin

Behavioral Ecology and Sociobiology, Год журнала: 2023, Номер 77(12)

Опубликована: Дек. 1, 2023

Язык: Английский

Процитировано

1
Egg-Clutch Biomechanics Affect Escape-Hatching Behavior and Performance DOI Creative Commons
Brandon A. Güell, J. Gregory McDaniel, Karen M. Warkentin

и другие.

Integrative Organismal Biology, Год журнала: 2024, Номер 6(1)

Опубликована: Янв. 1, 2024

Synopsis Arboreal embryos of phyllomedusine treefrogs hatch prematurely to escape snake predation, cued by vibrations in their egg clutches during attacks. However, success varies between species, from ∼77% Agalychnis callidryas just ∼9% A. spurrelli at 1 day premature. Both species begin responding attacks similar developmental stages, when vestibular mechanosensory function begins, suggesting that sensory ability does not limit the hatching response spurrelli. are thick and gelatinous, while thinner stiffer. We hypothesized this structural difference alters motion excited Since snakes must propagate through reach embryos, we attack-induced may reflect effects clutch biomechanics on cues available embryos. Mechanics predicts thinner, stiffer structures have higher free vibration frequencies, greater spatial attenuation, faster damping than thicker, more flexible structures. assessed embedding small accelerometers both recording standardized excitation tests two distances accelerometer. Analyses recorded showed frequencies clutches. Higher elicit less callidryas, could reduce amount can perceive. To directly test if structure affects attacks, transplanted eggs into compared rates with untransplanted, age-matched conspecific controls. also performed reciprocal transplantation pairs as a method control. Transplanting nearly tripled (44%) controls (15%), whereas transplanting different only increased 10%. At competence, no longer jelly-encapsulated, retain jelly coat. Therefore, latency de-jellied control, jelly-encapsulated siblings using manual egg-jiggling simulate predation cues. Embryos were likely hatched control siblings. Together, our results suggest properties parentally produced egg-clutch structures, including biomechanics, constrain information contribute interspecific differences responses predator

Язык: Английский

Процитировано

0
Social influences on embryonic behaviour and the developmental onset of embryonic acquired predator recognition in minnows DOI Creative Commons

Brooke Karasch,

Jessica Ward

Animal Behaviour, Год журнала: 2024, Номер 219, С. 123017 - 123017

Опубликована: Дек. 24, 2024

Язык: Английский

Процитировано

0
To hatch and hatch not: does heterochrony in onset of vestibular mechanosensing explain species differences in escape-hatching success of Agalychnis embryos in snake attacks? DOI Creative Commons
Brandon A. Güell, Karen M. Warkentin

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Авг. 10, 2023

Abstract Phyllomedusid treefrogs hatch prematurely to escape egg predation, but success varies among species. Snake attacks elicited 55% less escape-hatching in spontaneously hatching Agalychnis spurrelli than developed A. callidryas . use their vestibular system and, secondarily, lateral line sense physical disturbances that cue hatching. Since develop faster, we hypothesized heterochronic shifts the onset timing of mechanosensory function, relative ability, might explain lower response cues. To test this, compared timings hypoxia- and mechanosensory-cued (MCH) function developmental series both Across species, most sibships began responding each at same stage development, measured by vestibulo-ocular reflex (VOR), was similar. These results demonstrate low is not caused a delay function. MCH associated with VOR, higher threshold callidryas. The absence before strong suggests multimodal mechanosensing may contribute antipredator responses embryos. Our observations larger clutches snake complications flooded suggest differences clutch capsule structure directly species success. Moreover, select against false alarms, increasing stringency decision rules.

Язык: Английский

Процитировано

0