Greenlandic seed bugs cope well with heat DOI Open Access
Kathryn Knight

Journal of Experimental Biology, Год журнала: 2023, Номер 226(11)

Опубликована: Июнь 1, 2023

Creatures that live in the Arctic have a reputation for hardiness, weathering bitter winters and chilly summers. Even insects to deal with drastic temperature ranges summer ‘Coping such extremes is critical survival reproduction of these regions’, says Natasja Noer from Aalborg University, Denmark. But climate changing warming particularly alarming, record-setting heatwaves exceeding 30°C June 2022. How well will robust this rapidly heating region cope higher temperatures? Greenlandic seed bugs (Nysius groenlandicus) complete single life cycle during brief Nordic summer, so her colleagues Torsten Kristensen Simon Bahrndorff (Aalborg University) ventured Greenland late 2018 find out how warmer temperatures.Over 3-week period, team on five separate days collect 2.5–4 mm long Narsarsuaq, – where researchers were also keeping track continuously air retrieving early morning, midday, afternoon evening. The then either warmed bugs, quickly they would pass out, or plunged them into –3°C bath before rewarming them, soon recover chill.After reconstructing temperatures had experienced run-up tests, realised females conditions preceding hours better prepared sudden heatwave, whereas those cooler resuscitated most swiftly their bath. In contrast, male warm period 8–10 h chill took longer revive, weather prior unexpected heatwave did not improve ability withstand heat. short, female respond blasts heat, adjusting within 45 min, are less able rises. However, blast 12 earlier seems prepare both males later day.Next, Kåre Nielsen, Elsa Sverrisdóttir (both checked affected by conditions, determining which genes activating repressing (top ∼10°C) hot (∼22°C) day. As rose hotter day, increased activation more than 100 genes, especially morning. switching protection?This time, Toke Høye (Aarhus Denmark) delivered Narsarsuaq Aalborg, gradually 43°C cooled 3°C, discovering when hot, boosted protect proteins bodies heat damage as prioritizing egg production ensure future next generation. compared gene levels high home laboratory, wild seemed sensitive, protective at lower lab.So, reasonably bright night-time chills polar home, tracking adjust accordingly.

Язык: Английский

High and Low Temperatures Differentially Affect Survival, Reproduction, and Gene Transcription in Male and Female Moths of Spodoptera frugiperda DOI Creative Commons
Yi-Dong Tao, Yu Liu,

Xiao-Shuang Wan

и другие.

Insects, Год журнала: 2023, Номер 14(12), С. 958 - 958

Опубликована: Дек. 17, 2023

In this study, we found that both heat and cold stresses significantly affected the survival reproduction of sexes in Spodoptera frugiperda adults, with larvae showing relatively higher extreme temperature tolerance. Further transcriptomic analysis adults remarkable differences similarities between terms stress responses. Metabolism-related processes were suppressed stressed females, which did not occur to same extend males. Moreover, reduced immune activities sexes. Heat induced upregulation many shock proteins sexes, whereas response was insignificant. More tolerance-related genes, such as cuticle proteins, UDP-glucuronosyltransferase, facilitated trehalose transporter Tret1, upregulated males, most these genes downregulated females. a large number fatty acid-related acid synthases desaturases, differentially expressed under females zinc finger reproduction-related genes; downregulation linked reproduction. addition, TRPA1-like encoding (which have functions involved detecting changes) sex peptide receptor-like be moths. These results indicate sex-specific responses adaptive mechanisms suggest trade-offs stress-resistant progresses fundamental metabolic well

Язык: Английский

Процитировано

15
Acclimation to moderate temperatures can have strong negative impacts on heat tolerance of arctic arthropods DOI Creative Commons
Jesper Givskov Sørensen, Natasja Krog Noer, Torsten Nygaard Kristensen

и другие.

Functional Ecology, Год журнала: 2024, Номер 38(7), С. 1549 - 1561

Опубликована: Май 19, 2024

Abstract The Arctic is impacted by some of the fastest temperature changes observed on Earth, but impact terrestrial arthropod fauna unclear. Acute physiological thermal limits ectotherms from high latitudes often exceed local air temperatures, suggesting that they may be able to cope with increasing temperatures. However, knowledge how arthropods elevated temperatures for longer periods lacking. Here we investigate acclimation and exposure time affect acute heat tolerance five species ( Neomolgus littoralis , Megaphorura arctica Nysius groenlandicus Psammotettix lividellus Nabis flavomarginatus ) immediately after collection in sub‐Arctic habitats. We show although tolerances are relatively high, even moderate (temperature span assessed ca. 3–29°C) a 24 h period have strong negative effects four species. Similarly, negatively affected tolerance, depending temperature. Together our results suggest moderately or shorter can lead lower cold adapted regions. Consequently, climate change leading extended mildly these argue this aspect currently overlooked when assessing ability sub‐Artic regions as such predictions typically based estimates assumption beneficial responses. Read free Plain Language Summary article Journal blog.

Язык: Английский

Процитировано

3
A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest DOI Creative Commons
Xin Miao, Fang Cao, Xiaofei Yu

и другие.

Open Biology, Год журнала: 2025, Номер 15(4)

Опубликована: Апрель 1, 2025

Global climate change is characterized by increased extreme temperatures affecting insects at all trophic levels. Zinc finger proteins (ZFPs) are key regulators of gene expression and cell differentiation in eukaryotes, essential for stress resistance both animals plants. Using CRISPR/Cas9 deletion, this study predicted examined the structure ZFP320 diamondback moth ( Plutella xylostella ) investigated its function temperature response through a comprehensive age-stage, two-sex life table analysis. We found encodes 387 amino acid protein (43 kDa) with no transmembrane domains, featuring ZnF-C2H2 domain. Quantitative fluorescence analysis showed that under high temperatures. knockout altered antioxidant expression, resulting higher levels superoxide dismutase catalase mutant strains compared wild-type strain. Life revealed had shorter fecundity oviposition periods normal Additionally, exhibited lower parameters r , λ R 0 ), as well reduced survival rates critical thermal maxima. Notably, PxZFP320 plays crucial role adaptation, paving way future investigations on significance ZFPs P. ’s tolerance.

Язык: Английский

Процитировано

0
Comparative Transcriptome Analysis of Galeruca daurica Reveals Cold Tolerance Mechanisms DOI Open Access

Hongling Zhang,

Feilong Sun,

Wenbing Zhang

и другие.

Genes, Год журнала: 2023, Номер 14(12), С. 2177 - 2177

Опубликована: Дек. 4, 2023

Galeruca daurica (Joannis) is a pest species with serious outbreaks in the Inner Mongolian grasslands recent years, and its larvae eggs are extremely cold-tolerant. To gain deeper understanding of molecular mechanism cold-tolerant stress response, we performed de novo transcriptome assembly G. via RNA-Seq compared differentially expressed genes (DEGs) first- second-instar grown developed indoors outdoors, respectively. The results show that cold tolerance associated changes gene expression mainly involved glycolysis/gluconeogenesis pathway, fatty acid biosynthesis pathway production heat shock proteins (HSPs). Compared control group (indoor), gluconeogenesis, HSP were up-regulated outdoors. While these related to physiological metabolism growth insects, it was hypothesized encoded by play an important role insects. In addition, also investigated metabolic HSPs, HSP-related significantly outdoors indoor group. Finally, chose induce significant differences Hsp70 (Hsp70A1, Hsp70-2 Hsp70-3) RNAi further illustrate on larvae. separate mixed injections dsHSP70A1, dsHsp70-2 dsHsp70-3 reduced levels target super-cooling point (SCP) body fluid freezing (FP) test determined after using thermocouple method, found silencing increased SCP FP larvae, which validated resistance Our findings provide theoretical basis for excavating key response environments analyzing adaptation insects harsh environments.

Язык: Английский

Процитировано

4
Strong acclimation effect of temperature and humidity on heat tolerance of the Arctic collembolan Megaphorura arctica DOI Creative Commons
Sara Nørris Christoffersen, Cino Pertoldi, Jesper Givskov Sørensen

и другие.

Journal of Experimental Biology, Год журнала: 2024, Номер 227(12)

Опубликована: Июнь 6, 2024

The Arctic is a highly variable environment in which extreme daily and seasonal temperature fluctuations can occur. With climate change, an increase the occurrence of high temperatures drought events expected. While effects cold dehydration stress on polar arthropods are well studied combination, little known about how these species respond to combined heat stress. In this paper, we investigated tolerance collembola Megaphorura arctica affected by combinations different humidity acclimation regimes under controlled laboratory conditions. effect was complex dependent both time temperature, found have positive, negative or no depending experimental Further, marked interaction between tolerance, with lower severely decreasing when increased. This more pronounced increasing time. Lastly, fluctuating regime time, as levels. Together, results show that thermal alone has moderate but events, likely be frequent future, combination large impacts some arthropods.

Язык: Английский

Процитировано

1
Adaptive genomic variation is linked to a climatic gradient in a social wasp DOI Open Access

Hannah L. Cook,

Sara Miller, Gilbert Giri

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 14, 2023

ABSTRACT Species vary in their ability to adapt rapid changes, with the presence of genetic variation often facilitating long-term evolutionary responses. Given impending threat climate change, it is critical investigate how facilitates persistence and possible range expansion animals. Here, we combine genomic climatic data characterize drivers local adaptation widely distributed, social wasp Mischocyttarus mexicanus cubicola . Using whole genome sequence data, show that adaptive linked a gradient across broad distribution this species. We found strong population structure, dividing species into two clusters follow subtropical temperate regions. Patterns gene flow deviate from those expected by isolation distance alone differences resulting reduced even between adjacent populations. Importantly, genotype-environment analyses reveal candidate single nucleotide polymorphism (SNPs) associated temperature rainfall, suggesting for thermal desiccation tolerance. In particular, SNPs or near mitochondrial genes ND5 , CO1 COIII are cold tolerance metabolism. Similarly, Gld nuclear shown mediate hardiness cuticle formation, shows non-synonymous mutations unique Together, our results consistent distinct conditions. Thus, integration can be powerful approach predict vulnerability under change.

Язык: Английский

Процитировано

1
Differential expression of hemolymph proteins in wild bumblebees provides insights into species‐specific impacts of heat stress DOI
Kimberly Przybyla, Baptiste Martinet, Denis Michez

и другие.

Physiological Entomology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 27, 2024

Abstract Wildlife faces an increasing threat from extreme climatic events, such as heatwaves, which can have a severe impact on various species, including crucial pollinators like bumblebees. Bumblebees are cold‐adapted and heterothermic, possessing the ability to regulate their internal temperature. The of heat stress seems species specific in While most impacted, some bumblebee manage survive, potentially by employing physiological mechanisms, modulation protein profile (e.g. Heat Shock Proteins). However, there is limited understanding how profiles associated with exposure. In this study, we examined global variation males two sampled wild: heat‐tolerant Bombus terrestris heat‐sensitive magnus . After subjecting them stupor at 40°C controlled condition, it was observed that nearly all B. survived stress, while over 50% individuals succumbed Through off‐gel bottom‐up proteomics LC–MS/MS analysis hemolymph proteome, identified 164 proteins both large part differentially expressed after Additionally, quantitative fat bodies revealed relative mass stable , significantly lower exposed stress. Our data suggest compared displays higher adaptability its proteome response This could be key factor contributing high resistance adapt new, stressful environments expected due climate change. Understanding these mechanisms regulation bumblebees provide valuable insights into resilience vulnerability facing environmental stresses.

Язык: Английский

Процитировано

0
Nonlinear transcriptomic responses to compounded environmental changes across temperature and resources in a pest beetle, Callosobruchus maculatus (Coleoptera: Chrysomelidae) DOI Creative Commons
Beth A McCaw, Aoife Leonard, Lesley T. Lancaster

и другие.

Journal of Insect Science, Год журнала: 2024, Номер 24(6)

Опубликована: Ноя. 1, 2024

Abstract Many species are experiencing drastic and multidimensional changes to their environment due anthropogenic events. These may act nonadditively on physiological life history responses, thus not be predicted by responses single dimensional environmental changes. Therefore, work is needed understand species’ multiple aspects of change. We used whole-transcriptomic RNA-Sequencing assays uncover singly-applied shifts in resource or temperature dimensions, comparison combined, change, the crop pest seed beetle, Callosobruchus maculatus. found that change caused larger fecundity, developmental period offspring viability than additive effects 1-dimensional In addition, there was little overlap between genes differentially expressed under treatment versus altered conditions alone. Moreover, 115 exhibited significant × interaction expression, including those involved energy metabolism, detoxification, enhanced formation cuticle structural components. conclude alone cannot determine transcriptomic results highlight importance studying for understanding molecular phenotypic allow organisms insects rapidly adapt simultaneously

Язык: Английский

Процитировано

0
Greenlandic seed bugs cope well with heat DOI Open Access
Kathryn Knight

Journal of Experimental Biology, Год журнала: 2023, Номер 226(11)

Опубликована: Июнь 1, 2023

Creatures that live in the Arctic have a reputation for hardiness, weathering bitter winters and chilly summers. Even insects to deal with drastic temperature ranges summer ‘Coping such extremes is critical survival reproduction of these regions’, says Natasja Noer from Aalborg University, Denmark. But climate changing warming particularly alarming, record-setting heatwaves exceeding 30°C June 2022. How well will robust this rapidly heating region cope higher temperatures? Greenlandic seed bugs (Nysius groenlandicus) complete single life cycle during brief Nordic summer, so her colleagues Torsten Kristensen Simon Bahrndorff (Aalborg University) ventured Greenland late 2018 find out how warmer temperatures.Over 3-week period, team on five separate days collect 2.5–4 mm long Narsarsuaq, – where researchers were also keeping track continuously air retrieving early morning, midday, afternoon evening. The then either warmed bugs, quickly they would pass out, or plunged them into –3°C bath before rewarming them, soon recover chill.After reconstructing temperatures had experienced run-up tests, realised females conditions preceding hours better prepared sudden heatwave, whereas those cooler resuscitated most swiftly their bath. In contrast, male warm period 8–10 h chill took longer revive, weather prior unexpected heatwave did not improve ability withstand heat. short, female respond blasts heat, adjusting within 45 min, are less able rises. However, blast 12 earlier seems prepare both males later day.Next, Kåre Nielsen, Elsa Sverrisdóttir (both checked affected by conditions, determining which genes activating repressing (top ∼10°C) hot (∼22°C) day. As rose hotter day, increased activation more than 100 genes, especially morning. switching protection?This time, Toke Høye (Aarhus Denmark) delivered Narsarsuaq Aalborg, gradually 43°C cooled 3°C, discovering when hot, boosted protect proteins bodies heat damage as prioritizing egg production ensure future next generation. compared gene levels high home laboratory, wild seemed sensitive, protective at lower lab.So, reasonably bright night-time chills polar home, tracking adjust accordingly.

Язык: Английский

Процитировано

0