High and Low Temperatures Differentially Affect Survival, Reproduction, and Gene Transcription in Male and Female Moths of Spodoptera frugiperda
Insects,
Год журнала:
2023,
Номер
14(12), С. 958 - 958
Опубликована: Дек. 17, 2023
In
this
study,
we
found
that
both
heat
and
cold
stresses
significantly
affected
the
survival
reproduction
of
sexes
in
Spodoptera
frugiperda
adults,
with
larvae
showing
relatively
higher
extreme
temperature
tolerance.
Further
transcriptomic
analysis
adults
remarkable
differences
similarities
between
terms
stress
responses.
Metabolism-related
processes
were
suppressed
stressed
females,
which
did
not
occur
to
same
extend
males.
Moreover,
reduced
immune
activities
sexes.
Heat
induced
upregulation
many
shock
proteins
sexes,
whereas
response
was
insignificant.
More
tolerance-related
genes,
such
as
cuticle
proteins,
UDP-glucuronosyltransferase,
facilitated
trehalose
transporter
Tret1,
upregulated
males,
most
these
genes
downregulated
females.
a
large
number
fatty
acid-related
acid
synthases
desaturases,
differentially
expressed
under
females
zinc
finger
reproduction-related
genes;
downregulation
linked
reproduction.
addition,
TRPA1-like
encoding
(which
have
functions
involved
detecting
changes)
sex
peptide
receptor-like
be
moths.
These
results
indicate
sex-specific
responses
adaptive
mechanisms
suggest
trade-offs
stress-resistant
progresses
fundamental
metabolic
well
Язык: Английский
Acclimation to moderate temperatures can have strong negative impacts on heat tolerance of arctic arthropods
Functional Ecology,
Год журнала:
2024,
Номер
38(7), С. 1549 - 1561
Опубликована: Май 19, 2024
Abstract
The
Arctic
is
impacted
by
some
of
the
fastest
temperature
changes
observed
on
Earth,
but
impact
terrestrial
arthropod
fauna
unclear.
Acute
physiological
thermal
limits
ectotherms
from
high
latitudes
often
exceed
local
air
temperatures,
suggesting
that
they
may
be
able
to
cope
with
increasing
temperatures.
However,
knowledge
how
arthropods
elevated
temperatures
for
longer
periods
lacking.
Here
we
investigate
acclimation
and
exposure
time
affect
acute
heat
tolerance
five
species
(
Neomolgus
littoralis
,
Megaphorura
arctica
Nysius
groenlandicus
Psammotettix
lividellus
Nabis
flavomarginatus
)
immediately
after
collection
in
sub‐Arctic
habitats.
We
show
although
tolerances
are
relatively
high,
even
moderate
(temperature
span
assessed
ca.
3–29°C)
a
24
h
period
have
strong
negative
effects
four
species.
Similarly,
negatively
affected
tolerance,
depending
temperature.
Together
our
results
suggest
moderately
or
shorter
can
lead
lower
cold
adapted
regions.
Consequently,
climate
change
leading
extended
mildly
these
argue
this
aspect
currently
overlooked
when
assessing
ability
sub‐Artic
regions
as
such
predictions
typically
based
estimates
assumption
beneficial
responses.
Read
free
Plain
Language
Summary
article
Journal
blog.
Язык: Английский
A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest
Open Biology,
Год журнала:
2025,
Номер
15(4)
Опубликована: Апрель 1, 2025
Global
climate
change
is
characterized
by
increased
extreme
temperatures
affecting
insects
at
all
trophic
levels.
Zinc
finger
proteins
(ZFPs)
are
key
regulators
of
gene
expression
and
cell
differentiation
in
eukaryotes,
essential
for
stress
resistance
both
animals
plants.
Using
CRISPR/Cas9
deletion,
this
study
predicted
examined
the
structure
ZFP320
diamondback
moth
(
Plutella
xylostella
)
investigated
its
function
temperature
response
through
a
comprehensive
age-stage,
two-sex
life
table
analysis.
We
found
encodes
387
amino
acid
protein
(43
kDa)
with
no
transmembrane
domains,
featuring
ZnF-C2H2
domain.
Quantitative
fluorescence
analysis
showed
that
under
high
temperatures.
knockout
altered
antioxidant
expression,
resulting
higher
levels
superoxide
dismutase
catalase
mutant
strains
compared
wild-type
strain.
Life
revealed
had
shorter
fecundity
oviposition
periods
normal
Additionally,
exhibited
lower
parameters
r
,
λ
R
0
),
as
well
reduced
survival
rates
critical
thermal
maxima.
Notably,
PxZFP320
plays
crucial
role
adaptation,
paving
way
future
investigations
on
significance
ZFPs
P.
’s
tolerance.
Язык: Английский
Comparative Transcriptome Analysis of Galeruca daurica Reveals Cold Tolerance Mechanisms
Genes,
Год журнала:
2023,
Номер
14(12), С. 2177 - 2177
Опубликована: Дек. 4, 2023
Galeruca
daurica
(Joannis)
is
a
pest
species
with
serious
outbreaks
in
the
Inner
Mongolian
grasslands
recent
years,
and
its
larvae
eggs
are
extremely
cold-tolerant.
To
gain
deeper
understanding
of
molecular
mechanism
cold-tolerant
stress
response,
we
performed
de
novo
transcriptome
assembly
G.
via
RNA-Seq
compared
differentially
expressed
genes
(DEGs)
first-
second-instar
grown
developed
indoors
outdoors,
respectively.
The
results
show
that
cold
tolerance
associated
changes
gene
expression
mainly
involved
glycolysis/gluconeogenesis
pathway,
fatty
acid
biosynthesis
pathway
production
heat
shock
proteins
(HSPs).
Compared
control
group
(indoor),
gluconeogenesis,
HSP
were
up-regulated
outdoors.
While
these
related
to
physiological
metabolism
growth
insects,
it
was
hypothesized
encoded
by
play
an
important
role
insects.
In
addition,
also
investigated
metabolic
HSPs,
HSP-related
significantly
outdoors
indoor
group.
Finally,
chose
induce
significant
differences
Hsp70
(Hsp70A1,
Hsp70-2
Hsp70-3)
RNAi
further
illustrate
on
larvae.
separate
mixed
injections
dsHSP70A1,
dsHsp70-2
dsHsp70-3
reduced
levels
target
super-cooling
point
(SCP)
body
fluid
freezing
(FP)
test
determined
after
using
thermocouple
method,
found
silencing
increased
SCP
FP
larvae,
which
validated
resistance
Our
findings
provide
theoretical
basis
for
excavating
key
response
environments
analyzing
adaptation
insects
harsh
environments.
Язык: Английский
Strong acclimation effect of temperature and humidity on heat tolerance of the Arctic collembolan Megaphorura arctica
Journal of Experimental Biology,
Год журнала:
2024,
Номер
227(12)
Опубликована: Июнь 6, 2024
The
Arctic
is
a
highly
variable
environment
in
which
extreme
daily
and
seasonal
temperature
fluctuations
can
occur.
With
climate
change,
an
increase
the
occurrence
of
high
temperatures
drought
events
expected.
While
effects
cold
dehydration
stress
on
polar
arthropods
are
well
studied
combination,
little
known
about
how
these
species
respond
to
combined
heat
stress.
In
this
paper,
we
investigated
tolerance
collembola
Megaphorura
arctica
affected
by
combinations
different
humidity
acclimation
regimes
under
controlled
laboratory
conditions.
effect
was
complex
dependent
both
time
temperature,
found
have
positive,
negative
or
no
depending
experimental
Further,
marked
interaction
between
tolerance,
with
lower
severely
decreasing
when
increased.
This
more
pronounced
increasing
time.
Lastly,
fluctuating
regime
time,
as
levels.
Together,
results
show
that
thermal
alone
has
moderate
but
events,
likely
be
frequent
future,
combination
large
impacts
some
arthropods.
Язык: Английский
Adaptive genomic variation is linked to a climatic gradient in a social wasp
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 14, 2023
ABSTRACT
Species
vary
in
their
ability
to
adapt
rapid
changes,
with
the
presence
of
genetic
variation
often
facilitating
long-term
evolutionary
responses.
Given
impending
threat
climate
change,
it
is
critical
investigate
how
facilitates
persistence
and
possible
range
expansion
animals.
Here,
we
combine
genomic
climatic
data
characterize
drivers
local
adaptation
widely
distributed,
social
wasp
Mischocyttarus
mexicanus
cubicola
.
Using
whole
genome
sequence
data,
show
that
adaptive
linked
a
gradient
across
broad
distribution
this
species.
We
found
strong
population
structure,
dividing
species
into
two
clusters
follow
subtropical
temperate
regions.
Patterns
gene
flow
deviate
from
those
expected
by
isolation
distance
alone
differences
resulting
reduced
even
between
adjacent
populations.
Importantly,
genotype-environment
analyses
reveal
candidate
single
nucleotide
polymorphism
(SNPs)
associated
temperature
rainfall,
suggesting
for
thermal
desiccation
tolerance.
In
particular,
SNPs
or
near
mitochondrial
genes
ND5
,
CO1
COIII
are
cold
tolerance
metabolism.
Similarly,
Gld
nuclear
shown
mediate
hardiness
cuticle
formation,
shows
non-synonymous
mutations
unique
Together,
our
results
consistent
distinct
conditions.
Thus,
integration
can
be
powerful
approach
predict
vulnerability
under
change.
Язык: Английский
Differential expression of hemolymph proteins in wild bumblebees provides insights into species‐specific impacts of heat stress
Physiological Entomology,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 27, 2024
Abstract
Wildlife
faces
an
increasing
threat
from
extreme
climatic
events,
such
as
heatwaves,
which
can
have
a
severe
impact
on
various
species,
including
crucial
pollinators
like
bumblebees.
Bumblebees
are
cold‐adapted
and
heterothermic,
possessing
the
ability
to
regulate
their
internal
temperature.
The
of
heat
stress
seems
species
specific
in
While
most
impacted,
some
bumblebee
manage
survive,
potentially
by
employing
physiological
mechanisms,
modulation
protein
profile
(e.g.
Heat
Shock
Proteins).
However,
there
is
limited
understanding
how
profiles
associated
with
exposure.
In
this
study,
we
examined
global
variation
males
two
sampled
wild:
heat‐tolerant
Bombus
terrestris
heat‐sensitive
magnus
.
After
subjecting
them
stupor
at
40°C
controlled
condition,
it
was
observed
that
nearly
all
B.
survived
stress,
while
over
50%
individuals
succumbed
Through
off‐gel
bottom‐up
proteomics
LC–MS/MS
analysis
hemolymph
proteome,
identified
164
proteins
both
large
part
differentially
expressed
after
Additionally,
quantitative
fat
bodies
revealed
relative
mass
stable
,
significantly
lower
exposed
stress.
Our
data
suggest
compared
displays
higher
adaptability
its
proteome
response
This
could
be
key
factor
contributing
high
resistance
adapt
new,
stressful
environments
expected
due
climate
change.
Understanding
these
mechanisms
regulation
bumblebees
provide
valuable
insights
into
resilience
vulnerability
facing
environmental
stresses.
Язык: Английский
Nonlinear transcriptomic responses to compounded environmental changes across temperature and resources in a pest beetle, Callosobruchus maculatus (Coleoptera: Chrysomelidae)
Journal of Insect Science,
Год журнала:
2024,
Номер
24(6)
Опубликована: Ноя. 1, 2024
Abstract
Many
species
are
experiencing
drastic
and
multidimensional
changes
to
their
environment
due
anthropogenic
events.
These
may
act
nonadditively
on
physiological
life
history
responses,
thus
not
be
predicted
by
responses
single
dimensional
environmental
changes.
Therefore,
work
is
needed
understand
species’
multiple
aspects
of
change.
We
used
whole-transcriptomic
RNA-Sequencing
assays
uncover
singly-applied
shifts
in
resource
or
temperature
dimensions,
comparison
combined,
change,
the
crop
pest
seed
beetle,
Callosobruchus
maculatus.
found
that
change
caused
larger
fecundity,
developmental
period
offspring
viability
than
additive
effects
1-dimensional
In
addition,
there
was
little
overlap
between
genes
differentially
expressed
under
treatment
versus
altered
conditions
alone.
Moreover,
115
exhibited
significant
×
interaction
expression,
including
those
involved
energy
metabolism,
detoxification,
enhanced
formation
cuticle
structural
components.
conclude
alone
cannot
determine
transcriptomic
results
highlight
importance
studying
for
understanding
molecular
phenotypic
allow
organisms
insects
rapidly
adapt
simultaneously
Язык: Английский
Greenlandic seed bugs cope well with heat
Journal of Experimental Biology,
Год журнала:
2023,
Номер
226(11)
Опубликована: Июнь 1, 2023
Creatures
that
live
in
the
Arctic
have
a
reputation
for
hardiness,
weathering
bitter
winters
and
chilly
summers.
Even
insects
to
deal
with
drastic
temperature
ranges
summer
‘Coping
such
extremes
is
critical
survival
reproduction
of
these
regions’,
says
Natasja
Noer
from
Aalborg
University,
Denmark.
But
climate
changing
warming
particularly
alarming,
record-setting
heatwaves
exceeding
30°C
June
2022.
How
well
will
robust
this
rapidly
heating
region
cope
higher
temperatures?
Greenlandic
seed
bugs
(Nysius
groenlandicus)
complete
single
life
cycle
during
brief
Nordic
summer,
so
her
colleagues
Torsten
Kristensen
Simon
Bahrndorff
(Aalborg
University)
ventured
Greenland
late
2018
find
out
how
warmer
temperatures.Over
3-week
period,
team
on
five
separate
days
collect
2.5–4
mm
long
Narsarsuaq,
–
where
researchers
were
also
keeping
track
continuously
air
retrieving
early
morning,
midday,
afternoon
evening.
The
then
either
warmed
bugs,
quickly
they
would
pass
out,
or
plunged
them
into
–3°C
bath
before
rewarming
them,
soon
recover
chill.After
reconstructing
temperatures
had
experienced
run-up
tests,
realised
females
conditions
preceding
hours
better
prepared
sudden
heatwave,
whereas
those
cooler
resuscitated
most
swiftly
their
bath.
In
contrast,
male
warm
period
8–10
h
chill
took
longer
revive,
weather
prior
unexpected
heatwave
did
not
improve
ability
withstand
heat.
short,
female
respond
blasts
heat,
adjusting
within
45
min,
are
less
able
rises.
However,
blast
12
earlier
seems
prepare
both
males
later
day.Next,
Kåre
Nielsen,
Elsa
Sverrisdóttir
(both
checked
affected
by
conditions,
determining
which
genes
activating
repressing
(top
∼10°C)
hot
(∼22°C)
day.
As
rose
hotter
day,
increased
activation
more
than
100
genes,
especially
morning.
switching
protection?This
time,
Toke
Høye
(Aarhus
Denmark)
delivered
Narsarsuaq
Aalborg,
gradually
43°C
cooled
3°C,
discovering
when
hot,
boosted
protect
proteins
bodies
heat
damage
as
prioritizing
egg
production
ensure
future
next
generation.
compared
gene
levels
high
home
laboratory,
wild
seemed
sensitive,
protective
at
lower
lab.So,
reasonably
bright
night-time
chills
polar
home,
tracking
adjust
accordingly.
Язык: Английский