Journal of Comparative Physiology A,
Год журнала:
2023,
Номер
210(4), С. 585 - 599
Опубликована: Авг. 16, 2023
Abstract
Organisms
adapt
to
unfavorable
seasonal
conditions
survive.
These
adaptations
rely
on
the
correct
interpretation
of
environmental
cues
such
as
photoperiod,
and
temperature.
Genetic
studies
in
several
organisms,
including
genetic
powerhouse
Drosophila
melanogaster
,
indicate
that
circadian
clock
components,
period
timeless
are
involved
photoperiodic-dependent
adaptations,
but
our
understanding
this
process
is
far
from
complete.
In
particular,
role
temperature
a
key
factor
complement
photoperiodic
response
not
well
understood.
The
development
new
sequencing
technologies
has
proven
extremely
useful
plastic
changes
other
cellular
components
undergo
different
conditions,
gene
expression
alternative
splicing.
This
article
discusses
integration
photoperiod
for
biology
downstream
molecular
pathways
regulation
physiological
occur
with
changing
seasons.
We
focus
discussion
current
involvement
neuronal
circuits
these
D.
.
Insects,
Год журнала:
2025,
Номер
16(1), С. 54 - 54
Опубликована: Янв. 8, 2025
Insect
diapause
and
response
to
thermal
stress
are
similar
in
the
variety
of
manifestations.
However,
influence
shocks
on
incidence
insect
has
not
been
sufficiently
studied.
Our
laboratory
experiments
showed
that
both
cold
(-10
°C)
heat
(43
experienced
for
at
least
20-30
min
significantly
reduced
facultative
larval
winter
egg
parasitoid
Trichogramma
telengai.
patterns
these
responses
were
substantially
different.
In
particular,
peaks
sensitivity
diapause-averting
effects
fell,
correspondingly,
middle-stage
(5
days
development
15
late-stage
(9-11
larvae.
Heat
mostly
via
changes
initial
proportions
diapause-destined
non-diapause-destined
individuals,
whereas
effect
is
based
differential
mortality
(i.e.,
difference
among
treatments
same
experiment)
with
better
survival
individuals.
These
results
elucidate
peculiarities
interaction
between
diapause,
allowing
us
specify
methods
mass
rearing
storage.
Archives of Insect Biochemistry and Physiology,
Год журнала:
2025,
Номер
118(2)
Опубликована: Фев. 1, 2025
ABSTRACT
The
primary
vector
of
the
West
Nile
virus,
Culex
pipiens
,
undergoes
reproductive
dormancy
during
adverse
winter
season.
While
our
current
understanding
has
mainly
focused
on
cellular
signals
and
phenotypic
shifts
occurring
at
a
global
scale
diapause,
information
tissue‐specific
transcriptomic
changes
remains
limited.
This
knowledge
gap
is
major
challenge
in
interpreting
regulatory
mechanisms
tissue
level.
To
address
this,
present
work
utilized
RNA‐seq
technology
to
investigate
transcriptional
head
that
house
brain
crucial
endocrinal
organs
such
as
corpora
allata.
We
obtained
RNA
samples
from
heads
diapausing
nondiapausing
female
mosquitoes
two
specific
time
intervals,
ZT0
ZT16,
then
subjected
them
sequencing.
Our
results
revealed
differences
differentially
expressed
genes
between
diapause
non‐diapause
highlighting
diel
variations
gene
expression.
also
selected
twelve
associated
with
phenotype
examined
transcript
abundance
six
different
points
over
24
h.
qRT‐PCR
analysis
showed
similar
up‐
downregulation
transcripts
nondiapause
phenotypes
thus
validating
RNA‐seq.
In
summary,
study
identified
new
differentiation
their
expression,
potentially
linking
photoperiod
seasonal
insects.
newly
presented
will
significantly
advance
head‐specific
for
insect
diapause.
The
effects
of
environmental
stress
on
animal
life
are
gaining
importance
with
climate
change.
Diapause
is
a
dormancy
program
that
occurs
in
response
to
an
adverse
environment,
followed
by
resumption
development
and
reproduction
upon
the
return
favorable
conditions.
complex
trait,
so
we
leveraged
Drosophila
genetic
reference
panel
(DGRP)
lines
conducted
Genome-Wide
Association
Study
(GWAS)
characterize
basis
diapause.
We
assessed
post-diapause
non-diapause
fecundity
across
193
DGRP
lines.
GWAS
revealed
546
variants,
encompassing
single
nucleotide
polymorphisms,
insertions
deletions
associated
fecundity.
identified
291
candidate
diapause-associated
genes,
40
which
had
previously
been
diapause,
89
were
more
than
one
SNP.
Gene
network
analysis
indicated
genes
primarily
linked
neuronal
reproductive
system
development.
Similarly,
comparison
results
from
other
fly
greatest
overlap
olfactory-behavior-associated
fecundity-and-lifespan-associated
genes.
An
RNAi
screen
selected
candidates
two
Dip-𝛾
Scribbler,
be
required
during
recovery
for
complemented
test
neurons
successful
found
although
amputation
antenna
little
no
effect
lifespan,
it
reduced
diapause
lifespan
postdiapause
further
show
olfactory
receptor
temperature-sensing
Our
provide
insights
into
molecular,
cellular,
adult
.
Frontiers in Physiology,
Год журнала:
2025,
Номер
16
Опубликована: Март 14, 2025
Insect
diapause
is
a
vital
survival
strategy
that
enables
insects
to
enter
state
of
suspended
development,
allowing
them
withstand
unfavorable
environmental
conditions.
During
diapause,
significantly
lower
their
metabolic
rate
and
build
up
energy
reserves,
which
they
gradually
utilize
throughout
this
period.
The
regulation
involves
complex
interaction
hormones
enzymes.
Juvenile
(JHs)
affect
adults
larvae
differently;
in
adults,
the
absence
JH
typically
triggers
while
larvae,
presence
encourages
state.
Ecdysteroids,
regulate
molting
metamorphosis,
are
carefully
controlled
prevent
premature
development.
Reduced
signaling
insulin-like
peptides
enhances
stress
resistance
promotes
storage.
Several
enzymes
play
crucial
roles
adjustments
necessary
for
diapause.
These
include
degradation
JH,
ecdysteroidogenic
pathway,
metabolism
fatty
acids,
glycogen,
cryoprotectants,
responses.
Understanding
diapause's
molecular
biochemical
mechanisms
essential
fundamental
entomological
research
practical
applications.
Despite
recent
advances,
many
aspects
regulation,
especially
interactions
among
hormonal
pathways
role
enzymes,
remain
poorly
understood.
This
review
analyzes
approximately
250
papers
consolidate
current
knowledge
on
enzymatic
It
offers
comprehensive
overview
key
processes
based
studies
suggests
future
directions
fill
gaps
our
understanding
significant
biological
phenomenon.
also
lays
groundwork
enhancing
pest
control
strategies
ecological
conservation
by
deepening
mechanisms.
Biomolecules,
Год журнала:
2025,
Номер
15(4), С. 515 - 515
Опубликована: Апрель 1, 2025
Diapause
is
a
state
of
developmental
and
metabolic
dormancy
that
precedes
exposure
to
environmental
stresses.
Yet,
diapausing
embryos
are
typically
stress-tolerant.
Evidence
suggests
"prepare"
for
stress
as
part
gene
expression
program
they
enter
dormancy.
Here,
we
investigate
if
diapause
II
the
annual
killifish
Austrofundulus
limnaeus,
which
can
survive
hundreds
days
anoxia,
mount
transcriptomic
response
anoxic
insult.
Bulk
RNAseq
was
used
characterize
transcriptomes
exposed
normoxia,
4
h
24
2
normoxic
recovery
from
anoxia.
Differential
ontology
analyses
were
probe
pathways
may
mitigate
survival.
Transcriptional
factor
analysis
predict
potential
mediators
this
response.
Diapausing
exhibited
robust
anoxia
returns
near
baseline
conditions
after
h.
Anoxia
induced
an
upregulation
genes
involved
in
integrated
response,
lipid
metabolism,
p38mapk
kinase
signaling,
apoptosis.
Developmental
mitochondrial
decreased.
We
conclude
when
faced
with
This
consistent
mediating
expected
challenges
cellular
homeostasis
