Leveraging Transcriptional Signatures of Diverse Stressors for Bumble Bee Conservation DOI Creative Commons
Gabriela M. Quinlan, Heather M. Hines, Christina M. Grozinger

и другие.

Molecular Ecology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 13, 2024

ABSTRACT Organisms in nature are subjected to a variety of stressors, often simultaneously. Foremost among stressors key pollinators pathogens, poor nutrition and climate change. Landscape transcriptomics can be used decipher the relative role provided there unique signatures stress that reliably detected field specimens. In this study, we identify biomarkers bumble bee ( Bombus impatiens ) responses by first subjecting bees various short‐term (cold, heat, pathogen challenge) laboratory setting assessing their transcriptome responses. Using random forest classification on whole data, were able discriminate each stressor. Our best model (tissue‐specific trained subset important genes) correctly predicted known with 92% accuracy. We then applied wild‐caught sampled across heatwave event at two sites central Pennsylvania, US, expected differ baseline temperature floral resource availability. Transcriptomes during heat wave's peak showed stress, while collected relatively cooler morning periods starvation cold stress. failed pick up signals shortly after heatwave, suggesting set is more useful for identifying acute than long‐term monitoring chronic, landscape‐level stressors. highlight future directions fine‐tune landscape towards development better both conservation improving understanding stressor impacts bees.

Язык: Английский

The effect of seasonal temperatures on the physiology of the overwintered honey bee DOI Creative Commons
Olga Frunze,

Yumi Yun,

Hyunjee Kim

и другие.

PLoS ONE, Год журнала: 2024, Номер 19(12), С. e0315062 - e0315062

Опубликована: Дек. 9, 2024

Honey bee physiology follows an annual cycle, with winter bees living ten times longer than summer bees. Their transition can be disrupted by climate change. Several factors, mainly temperature, may contribute to the global losses of We simulated warming maintaining constant temperatures 25°C (Group 25) and 35°C 35) in rooms around hives from June October, while a Group control experienced natural conditions. Colony performance was assessed August September. In February, workers were examined for physiological traits (acinus size lipid content fat body) molecular markers ( vg JHAMT ), along potential ilp1 , ilp2 TOR1 HSP70 ). Our findings suggest that temperature decreases worker broods 25 fall led their different states related aging compared 35 workers. Changes end diapause detected upregulation genes. These signs response could lead development strategies prevent improve identification insect models.

Язык: Английский

Процитировано

0
Leveraging Transcriptional Signatures of Diverse Stressors for Bumble Bee Conservation DOI Creative Commons
Gabriela M. Quinlan, Heather M. Hines, Christina M. Grozinger

и другие.

Molecular Ecology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 13, 2024

ABSTRACT Organisms in nature are subjected to a variety of stressors, often simultaneously. Foremost among stressors key pollinators pathogens, poor nutrition and climate change. Landscape transcriptomics can be used decipher the relative role provided there unique signatures stress that reliably detected field specimens. In this study, we identify biomarkers bumble bee ( Bombus impatiens ) responses by first subjecting bees various short‐term (cold, heat, pathogen challenge) laboratory setting assessing their transcriptome responses. Using random forest classification on whole data, were able discriminate each stressor. Our best model (tissue‐specific trained subset important genes) correctly predicted known with 92% accuracy. We then applied wild‐caught sampled across heatwave event at two sites central Pennsylvania, US, expected differ baseline temperature floral resource availability. Transcriptomes during heat wave's peak showed stress, while collected relatively cooler morning periods starvation cold stress. failed pick up signals shortly after heatwave, suggesting set is more useful for identifying acute than long‐term monitoring chronic, landscape‐level stressors. highlight future directions fine‐tune landscape towards development better both conservation improving understanding stressor impacts bees.

Язык: Английский

Процитировано

0