Damsels in a Hidden Colour: Development of Ultraviolet Sensitivity and Colour Patterns in Damselfishes (Pomacentridae) DOI Creative Commons
Valerio Tettamanti, N. Justin Marshall, Karen L. Cheney

и другие.

Опубликована: Окт. 8, 2024

Damselfishes (Pomacentridae) are widespread and highly abundant on tropical coral reefs. They exhibit diverse body colouration within between the ∼250 species across ontogenetic stages. In addition to human visible colours (i.e., 400-700 nm), most adult damselfishes reflect ultraviolet (UV, 300-400 nm) colour patches. UV sensitivity signals essential for feeding form basis a secret communication channel invisible many UV-blind predatory fish reef; however, how these traits develop stages, their distribution damselfish family is poorly characterised. Here, we used photography, phylogenetic reconstructions of opsin genes, differential gene expression analysis (DGE) retinal samples, investigate development vision patterns in three stages (pre-settlement larval, juvenile, adult) eleven species. Using DGE, found similar juveniles adults, which strongly differed from larvae. All all expressed at least one UV-sensitive _sws1_ gene. However, only started appear juvenile stage. Moreover, _Pomacentrus_ displayed complex that were correlated with two copies. This could mean some can discriminate change component. We demonstrate dramatic shifts both damselfish, while highlighting importance considering ontogeny when studying coevolution visual systems signals.

Язык: Английский

Ultraviolet vision in anemonefish improves colour discrimination DOI Creative Commons
Laurie J. Mitchell,

Amelia M. Phelan,

Fabio Cortesi

и другие.

Journal of Experimental Biology, Год журнала: 2024, Номер 227(7)

Опубликована: Апрель 1, 2024

ABSTRACT In many animals, ultraviolet (UV) vision guides navigation, foraging, and communication, but few studies have addressed the contribution of UV signals to colour vision, or measured discrimination thresholds using behavioural experiments. Here, we tested in an anemonefish (Amphiprion ocellaris) a five-channel (RGB-V-UV) LED display. We first determined that maximal sensitivity A. ocellaris cone was ∼386 nm microspectrophotometry. Three additional spectral sensitivities had maxima at ∼497, 515 ∼535 nm. then behaviourally by training distinguish coloured target pixel from grey distractor pixels varying intensity. Thresholds were calculated for nine sets colours with without signals. Using tetrachromatic model, found better (i.e. lower) discriminating when higher chromatic contrast. These caused greater stimulation relative other types. findings imply component cues improves their detectability, which likely increases prominence body patterns communication silhouette zooplankton prey.

Язык: Английский

Процитировано

9

Damsels in Disguise: Development of Ultraviolet Sensitivity and Colour Patterns in Damselfishes (Pomacentridae) DOI Creative Commons
Valerio Tettamanti, N. Justin Marshall, Karen L. Cheney

и другие.

Molecular Ecology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

ABSTRACT Damselfishes (Pomacentridae) are widespread and highly abundant on tropical coral reefs. They exhibit diverse body colouration within between the ~250 species across ontogenetic stages. In addition to human‐visible colours (i.e., 400–700 nm), most adult damselfishes reflect ultraviolet (UV, 300–400 nm) colour patches. UV sensitivity signals essential for feeding form basis a secret communication channel invisible many UV‐blind predatory fish reef; however, how these traits develop stages their distribution damselfish family is poorly characterised. Here, we used photography, phylogenetic reconstructions of opsin genes, differential gene expression analysis (DGE) retinal samples investigate development vision patterns in three (pre‐settlement larval, juvenile, adult) 11 species. Using DGE, found similar juveniles adults, which strongly differed from larvae. All all expressed at least one UV‐sensitive sws1 gene. However, only started appear juvenile stage. Moreover, Pomacentrus displayed complex that were correlated with two copies. This could mean some can discriminate change component. We demonstrate dramatic shifts both while highlighting importance considering ontogeny when studying coevolution visual systems signals.

Язык: Английский

Процитировано

0

Bioinspired Motion Perception Using the Wavelength-Sensitive Nonvolatile MoTe2 Homojunction DOI

Haoxiang Tian,

Yi Cui, Miao Zhang

и другие.

ACS Photonics, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

0

Damsels in a hidden colour: development of ultraviolet sensitivity and colour patterns in damselfishes (Pomacentridae) DOI Open Access
Valerio Tettamanti, Justin Marshall, Karen L. Cheney

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 23, 2024

Damselfishes (Pomacentridae) are widespread and highly abundant on tropical coral reefs. They exhibit diverse body colouration within between the ~250 species across ontogenetic stages. In addition to human visible colours (i.e., 400-700 nm), most adult damselfishes reflect ultraviolet (UV, 300-400 nm) colour patches. UV sensitivity signals essential for feeding form basis a secret communication channel invisible many UV-blind predatory fish reef; however, how these traits develop stages, their distribution damselfish family is poorly characterised. Here, we used photography, phylogenetic reconstructions of opsin genes, differential gene expression analysis (DGE) retinal samples, investigate development vision patterns in three stages (pre-settlement larval, juvenile, adult) eleven species. Using DGE, found similar juveniles adults, which strongly differed from larvae. All all expressed at least one UV-sensitive sws1 gene. However, only started appear juvenile stage. Moreover, Pomacentrus displayed complex that were correlated with two copies. This could mean some can discriminate change component. We demonstrate dramatic shifts both damselfish, while highlighting importance considering ontogeny when studying coevolution visual systems signals.

Язык: Английский

Процитировано

1

Generation of the First Transgenic Line of the Iconic Coral Reef FishAmphiprion ocellaris DOI Creative Commons

Gabriel J. Graham,

Emma M. Ibanez, Laurie J. Mitchell

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 9, 2024

Abstract The common clownfish, Amphiprion ocellaris , is an iconic coral reef fish, ubiquitous in the marine aquarium hobby and useful for studying a variety of biological processes (e.g., mutual symbiosis, ultraviolet vision, protandrous sex change). Recently, CRISPR/Cas9 methods were developed knocking out specific genes mechanistic studies. Here, we expand genetic toolkit A. by creating first transgenic line using Tol2 transposon system. Fertilized eggs co-injected with transposase mRNA plasmid encoding Elongation factor 1 α ( Ef1α ): Green fluorescent protein GFP) cassette at various concentrations, needle tip dimensions timepoints post-fertilization. We compared injection parameters sterilization to maximize survival injected eggs. F0s (n=10) that genotyped GFP+ then raised 6 months age crossed wild-type (WT) females confirm germline transmission. F1 offspring also same manner. highly efficient system resulted 37% rate transgenesis surviving amounting 2.7% yield all being (n= 160). Of these, 10 adulthood, 8 spawned, 5/8 (62.5 %) produced offspring. Further, two F1s WT 53.8% 54.2% respectively, confirming creation stable line. This is, our knowledge, generation any fish. ability express transgenes interest anemonefish opens door new era exploration into their fascinating biology.

Язык: Английский

Процитировано

0

Generation of the First Transgenic Line of the Iconic Coral Reef Fish Amphiprion ocellaris DOI

Gabriel J. Graham,

Emma M. Ibanez, Laurie J. Mitchell

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июль 19, 2024

Abstract The common clownfish, Amphiprion ocellaris, is an iconic coral reef fish, ubiquitous in the marine aquarium hobby and useful for studying a variety of biological processes (e.g., mutual symbiosis, ultraviolet vision, protandrous sex change). Recently, CRISPR/Cas9 methods were developed knocking out specific genes mechanistic studies. Here, we expand genetic toolkit A. ocellaris by creating first transgenic line using Tol2 transposon system. Fertilized eggs co-injected with transposase mRNA plasmid encoding Elongation factor 1 α (Ef1α): Green fluorescent protein (GFP) cassette at various concentrations, needle tip dimensions timepoints post-fertilization. We compared injection parameters sterilization to maximize survival injected eggs. F0s (n = 10) that genotyped GFP + then raised 6 months age crossed wild-type (WT) females confirm germline transmission. F1 offspring also same manner. highly efficient system resulted 37% rate transgenesis surviving amounting 2.7% yield all being GFP+ 160). Of these, 10 adulthood, 8 spawned, 5/8 (62.5%) produced offspring. Further, two F1s WT 53.8% 54.2% respectively, confirming creation stable line. This is, our knowledge, generation any fish. ability express transgenes interest anemonefish opens door new era exploration into their fascinating biology.

Язык: Английский

Процитировано

0

Generation of the First Transgenic Line of the Iconic Coral Reef Fish Amphiprion ocellaris DOI

Gabriel J. Graham,

Emma M. Ibanez, Laurie J. Mitchell

и другие.

Marine Biotechnology, Год журнала: 2024, Номер 26(5), С. 1067 - 1078

Опубликована: Авг. 19, 2024

Язык: Английский

Процитировано

0

Coral reef fish visual adaptations to a changing world DOI Creative Commons
Abigail Shaughnessy, Fabio Cortesi

Functional Ecology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Abstract Coral reef ecosystems show fluctuations in their prevailing light environment response to both regular (e.g. between seasons) and more prevalent stochastic events human‐induced sediment runoff). In these shifting environments, phenotypic plasticity provides an essential mechanism for coral fishes adjust visual capability meet changing sensory requirements. Here, we evaluate the growing area of research that highlights many genetic ecological mechanisms affect plastic responses fish vision environmental cues. With increasing number disturbances marine environment, it is critical understand extent limits under natural disturbed conditions. our current knowledge drawing upon a large body work freshwater fishes, speculate whether can adapt changes where limitations could lie. Whilst have shown adaptations different degree inconsistent species. Thus, may not only be functionally significant maintaining performance visually guided behaviours single species but, broadly, likely key sustaining ecosystem function. Read free Plain Language Summary this article on Journal blog.

Язык: Английский

Процитировано

0

Damsels in a Hidden Colour: Development of Ultraviolet Sensitivity and Colour Patterns in Damselfishes (Pomacentridae) DOI Creative Commons
Valerio Tettamanti, N. Justin Marshall, Karen L. Cheney

и другие.

Опубликована: Окт. 8, 2024

Damselfishes (Pomacentridae) are widespread and highly abundant on tropical coral reefs. They exhibit diverse body colouration within between the ∼250 species across ontogenetic stages. In addition to human visible colours (i.e., 400-700 nm), most adult damselfishes reflect ultraviolet (UV, 300-400 nm) colour patches. UV sensitivity signals essential for feeding form basis a secret communication channel invisible many UV-blind predatory fish reef; however, how these traits develop stages, their distribution damselfish family is poorly characterised. Here, we used photography, phylogenetic reconstructions of opsin genes, differential gene expression analysis (DGE) retinal samples, investigate development vision patterns in three stages (pre-settlement larval, juvenile, adult) eleven species. Using DGE, found similar juveniles adults, which strongly differed from larvae. All all expressed at least one UV-sensitive _sws1_ gene. However, only started appear juvenile stage. Moreover, _Pomacentrus_ displayed complex that were correlated with two copies. This could mean some can discriminate change component. We demonstrate dramatic shifts both damselfish, while highlighting importance considering ontogeny when studying coevolution visual systems signals.

Язык: Английский

Процитировано

0