Bioinformatics for Inosine: Tools and Approaches to Trace This Elusive RNA Modification DOI Open Access
Enrico Bortoletto, Umberto Rosani

Genes, Год журнала: 2024, Номер 15(8), С. 996 - 996

Опубликована: Июль 29, 2024

Inosine is a nucleotide resulting from the deamination of adenosine in RNA. This chemical modification process, known as RNA editing, typically mediated by family double-stranded binding proteins named Adenosine Deaminase Acting on dsRNA (ADAR). While presence ADAR orthologs has been traced throughout evolution metazoans, existence and extension editing have characterized more limited number animals so far. Undoubtedly, ADAR-mediated plays vital role physiology, organismal development disease, making understanding evolutionary conservation this phenomenon pivotal to deep characterization relevant biological processes. However, lack direct high-throughput methods reveal modifications at single resolution an extended investigation editing. Nowadays, these developed, appropriate bioinformatic pipelines are required fully exploit data, which can complement existing approaches detect Here, we review current literature "bioinformatics for inosine" subject discuss future research avenues field.

Язык: Английский

Leveraging genetics to understand ADAR1-mediated RNA editing in health and disease DOI
Jin Billy Li, Carl R. Walkley

Nature Reviews Genetics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Язык: Английский

Процитировано

0
Advances in Detection Methods for A‐to‐I RNA Editing DOI Creative Commons
Yuxi Yang, Masayuki Sakurai

Wiley Interdisciplinary Reviews - RNA, Год журнала: 2025, Номер 16(2)

Опубликована: Март 1, 2025

ABSTRACT Adenosine‐to‐inosine (A‐to‐I) RNA editing is a key post‐transcriptional modification that influences gene expression and various cellular processes. Advances in sequencing technologies have greatly contributed to the identification of A‐to‐I sites, providing insights into their distribution across coding non‐coding regions. These developments facilitated discovery functionally relevant events advanced understanding biological roles. This review presents evolution methodologies for detection examines recent advances, including chemically‐assisted, enzyme‐assisted, quantitative approaches. By evaluating these techniques, we aim help researchers select most effective tools investigating its broader implications health disease.

Язык: Английский

Процитировано

0
Bioinformatics for Inosine: Tools and Approaches to Trace This Elusive RNA Modification DOI Open Access
Enrico Bortoletto, Umberto Rosani

Genes, Год журнала: 2024, Номер 15(8), С. 996 - 996

Опубликована: Июль 29, 2024

Inosine is a nucleotide resulting from the deamination of adenosine in RNA. This chemical modification process, known as RNA editing, typically mediated by family double-stranded binding proteins named Adenosine Deaminase Acting on dsRNA (ADAR). While presence ADAR orthologs has been traced throughout evolution metazoans, existence and extension editing have characterized more limited number animals so far. Undoubtedly, ADAR-mediated plays vital role physiology, organismal development disease, making understanding evolutionary conservation this phenomenon pivotal to deep characterization relevant biological processes. However, lack direct high-throughput methods reveal modifications at single resolution an extended investigation editing. Nowadays, these developed, appropriate bioinformatic pipelines are required fully exploit data, which can complement existing approaches detect Here, we review current literature "bioinformatics for inosine" subject discuss future research avenues field.

Язык: Английский

Процитировано

0