Identification of novel functional compounds from forest onion and its biological activities against breast cancer DOI Creative Commons
Fahrul Nurkolis, Isma Kurniatanty, Elvan Wiyarta

и другие.

Journal of Agriculture and Food Research, Год журнала: 2024, Номер 18, С. 101362 - 101362

Опубликована: Авг. 23, 2024

The discovery of new molecules from natural sources for the treatment tumors such as breast cancers is importance development functional foods and to pharmaceutical industry. A resource with potential activity against cancer forest onion, Eleutherine bulbosa (Mill.) Urb., but identity its active constituents their mechanisms action remain unexplored. Therefore, this study focuses on metabolite profiling, in silico or pharmacoinformatic mechanisms, well advanced validation vitro cell lines. Ten compounds identified E. bulb ethanolic extract (EBE) showed receptor radical inhibitory via network pharmacology molecular docking simulation. most promising compound was avenasterol binding PARP-1, HER2, iNOS receptors values −11.26, −8.34, −9.17 μg/mL, respectively. EBE had a smaller EC50 value, higher potency, than control antioxidant Trolox scavenging tests ABTS DPPH. In line study, antiproliferative human MCF-7 LD50 217.8 relatively low cytotoxicity normal MCF-10A cells (LD50 > 1000 μg/mL). mechanism associated downregulation TGF-β, PI3K, AKT which are known tumor activators. Significant (p < 0.05) upregulation suppressor gene miR-29a-3p observed after dose-dependent manner.

Язык: Английский

Novel Functional Food Properties of Forest Onion (Eleutherine bulbosa Merr.) Phytochemicals for Treating Metabolic Syndrome: New Insights from a Combined Computational and In Vitro Approach DOI Open Access
Happy Kurnia Permatasari, Nuril Farid Abshori, Rony Abdi Syahputra

и другие.

Nutrients, Год журнала: 2024, Номер 16(10), С. 1441 - 1441

Опубликована: Май 10, 2024

Metabolic syndrome is a global health problem. The use of functional foods as dietary components has been increasing. One food interest forest onion extract (FOE). This study aimed to investigate the effect FOE on lipid and glucose metabolism in silico vitro using 3T3-L1 mouse cell line. was comprehensive that used multi-modal computational network pharmacology analysis molecular docking cells vitro. phytochemical were analyzed untargeted ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS). Next, an performed determine FOE’s bioactive compounds, toxicity analysis, protein target identification, pharmacology, carried out. pancreatic lipase, α-glucosidase, α-amylase inhibition determined. Finally, we determined its accumulation MAPK8, PPARG, HMGCR, CPT-1, GLP1 expression preadipocyte We showed potential metabolites targeted inhibited lipase levels, Furthermore, significantly (p < 0.05) inhibits expressions GLP-1 dose-dependent manner. contains several reduce α-amylase, proteins associated with

Язык: Английский

Процитировано

2
Mechanism of Action of Isoflavone Derived from Soy-Based Tempeh as an Antioxidant and Breast Cancer Inhibitor via Potential Upregulation of miR-7-5p: A Multimodal Analysis Integrating Pharmacoinformatics and Cellular Studies DOI Creative Commons
Fahrul Nurkolis, Nurpudji Astuti Taslim, Dain Lee

и другие.

Antioxidants, Год журнала: 2024, Номер 13(6), С. 632 - 632

Опубликована: Май 22, 2024

Breast cancer presents a significant global health challenge with rising incidence rates worldwide. Despite current efforts, it remains inadequately controlled. Functional foods, notably tempeh, have emerged as promising candidates for breast prevention and treatment due to bioactive peptides isoflavones exhibiting potential anticancer properties by serving antioxidants, inducing apoptosis, inhibiting cell proliferation. This study integrates pharmacoinformatics cellular investigations (i.e., multifaceted approach) elucidate the antioxidative anti-breast of tempeh-derived isoflavones. Methodologies encompass metabolomic profiling, in silico analysis, antioxidant assays, vitro experiments. Daidzein genistein exhibited therapeutic options agents. In studies also supported their efficacy against ability scavenge radicals, particularly soy-based tempeh powder (SBT-P) its isoflavone derivatives. Results demonstrated downregulation signaling proteins increased expression miR-7-5p, microRNA tumor-suppressive properties. Notably, LD50 values SBT-P derivatives on normal lines indicate safety, minimal cytotoxic effects MCF-10A cells compared control groups. The underscores favorable safe option treatment, warranting further clinical exploration.

Язык: Английский

Процитировано

1
Identification of novel functional compounds from forest onion and its biological activities against breast cancer DOI Creative Commons
Fahrul Nurkolis, Isma Kurniatanty, Elvan Wiyarta

и другие.

Journal of Agriculture and Food Research, Год журнала: 2024, Номер 18, С. 101362 - 101362

Опубликована: Авг. 23, 2024

The discovery of new molecules from natural sources for the treatment tumors such as breast cancers is importance development functional foods and to pharmaceutical industry. A resource with potential activity against cancer forest onion, Eleutherine bulbosa (Mill.) Urb., but identity its active constituents their mechanisms action remain unexplored. Therefore, this study focuses on metabolite profiling, in silico or pharmacoinformatic mechanisms, well advanced validation vitro cell lines. Ten compounds identified E. bulb ethanolic extract (EBE) showed receptor radical inhibitory via network pharmacology molecular docking simulation. most promising compound was avenasterol binding PARP-1, HER2, iNOS receptors values −11.26, −8.34, −9.17 μg/mL, respectively. EBE had a smaller EC50 value, higher potency, than control antioxidant Trolox scavenging tests ABTS DPPH. In line study, antiproliferative human MCF-7 LD50 217.8 relatively low cytotoxicity normal MCF-10A cells (LD50 > 1000 μg/mL). mechanism associated downregulation TGF-β, PI3K, AKT which are known tumor activators. Significant (p < 0.05) upregulation suppressor gene miR-29a-3p observed after dose-dependent manner.

Язык: Английский

Процитировано

1