A column-switching two-dimensional liquid chromatography - high resolution/triple quadrupole dual mass spectrometry system for simultaneously untargeted metabolome and targeted exposome analyses DOI Creative Commons
Guowang Xu, Yuting Wang, Penglong Cao

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Фев. 3, 2025

Abstract Metabolome-exposome association studies (mEWAS) demands high-coverage and high-sensitivity analytical methods for metabolites exposures with diverse chemical properties concentrations. Here, we present an integrated column-switching two-dimensional liquid chromatography (2DLC) - high resolution/triple quadrupole dual mass spectrometry (MS) method, achieving simultaneously untargeted metabolome targeted exposome analyses including 598 in a single run. Polar non-polar compounds log P ranging from -8 to 15 were separated without unretained the dead time. Benchmarked against biological matrices, analyte coverage increased by 35-104% over one-dimensional LC method. Highly sensitive limits of detection 0.001-1 μg/mL >90% ng/mL obtained, matching concentration difference between human blood. The method was demonstrated patients chronic diseases, showing feasibility establishing mEWAS integrating 2DLC-dual MS techniques.

Язык: Английский

A column-switching two-dimensional liquid chromatography - high resolution/triple quadrupole dual mass spectrometry system for simultaneously untargeted metabolome and targeted exposome analyses DOI Creative Commons
Guowang Xu, Yuting Wang, Penglong Cao

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Фев. 3, 2025

Abstract Metabolome-exposome association studies (mEWAS) demands high-coverage and high-sensitivity analytical methods for metabolites exposures with diverse chemical properties concentrations. Here, we present an integrated column-switching two-dimensional liquid chromatography (2DLC) - high resolution/triple quadrupole dual mass spectrometry (MS) method, achieving simultaneously untargeted metabolome targeted exposome analyses including 598 in a single run. Polar non-polar compounds log P ranging from -8 to 15 were separated without unretained the dead time. Benchmarked against biological matrices, analyte coverage increased by 35-104% over one-dimensional LC method. Highly sensitive limits of detection 0.001-1 μg/mL >90% ng/mL obtained, matching concentration difference between human blood. The method was demonstrated patients chronic diseases, showing feasibility establishing mEWAS integrating 2DLC-dual MS techniques.

Язык: Английский

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