Molecular identification and antimicrobial potential of endophytic fungi against some grapevine pathogens
PLoS ONE,
Год журнала:
2024,
Номер
19(10), С. e0309041 - e0309041
Опубликована: Окт. 24, 2024
Endophytic
fungi
are
microorganisms
that,
exhibiting
within
the
plant
tissues
without
causing
any
apparent
harm
to
host,
establish
a
symbiotic
relationship
with
plants.
Host
plants
provide
endophytic
essential
nutrients
and
protected
environment.
In
exchange,
can
enhance
plant’s
ability
acquire
nutrients.
They
also
play
crucial
role
in
increasing
host
tolerance
various
abiotic
biotic
stresses.
produce
wide
range
of
bioactive
compounds,
some
similar
those
found
plant.
Iraq’s
Duhok
province
Kurdistan
region,
species
Vitis
vinifera
has
been
explored
as
habitat
for
diverse
across
ecological
environments.
During
period
from
2021
2022,
total
600
samples
were
collected
four
distinct
locations:
Bagera,
Besfke,
Barebhar,
Atrush.
From
these
samples,
twelve
fungal
isolated,
including
Aspergillus
flavipes
,
Botryosphaeria
dothidea
Fusarium
oxysporum
ruscicol
venenatum
Chaetomium
globosum
Clonostachys
rosea
Mucor
racemosus
Penicillium
glabrum
terreus
nidulans
niger
Alternaria
alternata
Paecilomyces
maximus
Curvularia
buchloes
.
These
introduced
their
potential
biocontrol
agents
against
grapevine
trunk
diseases
grape
rotting
fungi,
which
pose
significant
risks
health
productivity.
Penicilium
radiatolobatum
Botrysphaeria
ruscicola
represented
first
record
endophytes
on
Iraq.
Based
ITS
SSU
sequencing,
molecular
identification
confirmed
fungi’s
presence
sequence
identities
ranging
99%
100%.
Phylogenetic
analysis
revealed
that
could
be
categorized
into
five
main
clusters
(A,
B,
C,
D,
E),
showing
high
intra-group
similarity.
Utilizing
Dual
Culture
method,
endophyte
demonstrated
70.83%
inhibition
rate
Ilyonectria
destructans
Food
Poisoning
A
P
emerged
most
effective
inhibitors
whereas
M
achieved
complete
(100%)
Botrytis
cinerea
Additionally,
was
identified
agent
Neoscytalidium
dimidiatum
conclusion,
study
emphasizes
diseases,
highlighting
sustainable
vineyard
management.
findings
lead
further
exploration
implementation
fungi-inserted
pest
management
strategies.
Язык: Английский
Morphogenetic identification of new endophytic fungal species related to the family of Plectosphaerellaceae in Iraq
Bionatura journal :,
Год журнала:
2024,
Номер
1(1), С. 1 - 11
Опубликована: Март 15, 2024
This
study
focused
on
isolating
and
identifying
endophytic
fungi
from
vegetable
crops
in
Iraq.
Samples
seven
plants,
including
Anethum
graveolens,
Apium
Capsicum
annuum,
Malva
parviflora,
Mentha
piperita,
Petroselinum
sativum,
Portulaca
oleracea,
were
collected
five
central
regions
Basrah,
Iraq,
(Abu
Al-Khaseeb,
Karmat
Ali,
AL-Zubair,
Shatt
Al-Arab
the
Centre
of
Basrah).
Samples,
mature
leaves,
stems
roots
sources,
treated
laboratory.
Recovered
purified
identified
based
their
macro
micromorphological
features.
Identification
was
validated
by
DNA
sequencing
PCR
amplification
ITS4
ITS5
gene
primers
molecular
analysis.
Phylogenetic
examination
indicated
that
three
novel
fungal
species
are
documented
Iraqi
mycobiota
for
first
time,
isolated
plants
Basrah
province
related
to
family
Plectosphaerellaceae,
Gibellulopsis
serrae,
Plectosphaerella
niemeijerarum,
P.
oratosquillae.
Brief
descriptions
photo
panels
provided
newly
recorded
this
study.
These
findings
essential
understanding
community
within
parts
can
be
used
manage
control
plant
disease
enhance
productivity.
Keywords:
Ascomycota,
Bsarah,
fungi,
Plectosphaerellaceae.
Язык: Английский