Advanced Surface-Enhanced Raman Scattering Nanoprobes for Precise Detection of Nitroreductase in Hypoxic Tumor Cells: Improving Cancer Diagnosis DOI Creative Commons
Xiaoyue Zhao, Ying Zhang, Chunyan Zhu

и другие.

SLAS TECHNOLOGY, Год журнала: 2024, Номер unknown, С. 100229 - 100229

Опубликована: Дек. 1, 2024

Nitroreductase (NTR) plays a critical role in the oxygen-deficient environment of anoxic tumor cells, and its identification is crucial for diagnosis treatment cancer. This research introduces an innovative Surface Enhanced Raman Scattering (SERS) probe, created by attaching p-nitrothiophenol (p-NTP) to gold nanoparticles (Au NPs). probe leverages specific enzymatic reaction NTR hypoxic status, utilizing decreased NADH. The activity transforms nitroaromatic compounds into aromatic amines, which then reflected as measurable shift SERS signal probe. novel approach allows accurate quantification NTR, with sensitivity reaching detection threshold less than 0.02 μg/mL. probe's non-toxic nature superior biocompatibility facilitate use direct investigations A549 cells under reduced oxygen levels. We also applied this method xenograft model. results demonstrate marked increase levels tissues conditions, highlighting significance nanoprobe enhancing cancer diagnostics, helping medical doctors making decisions more swiftly effectively.

Язык: Английский

Advanced Surface-Enhanced Raman Scattering Nanoprobes for Precise Detection of Nitroreductase in Hypoxic Tumor Cells: Improving Cancer Diagnosis DOI Creative Commons
Xiaoyue Zhao, Ying Zhang, Chunyan Zhu

и другие.

SLAS TECHNOLOGY, Год журнала: 2024, Номер unknown, С. 100229 - 100229

Опубликована: Дек. 1, 2024

Nitroreductase (NTR) plays a critical role in the oxygen-deficient environment of anoxic tumor cells, and its identification is crucial for diagnosis treatment cancer. This research introduces an innovative Surface Enhanced Raman Scattering (SERS) probe, created by attaching p-nitrothiophenol (p-NTP) to gold nanoparticles (Au NPs). probe leverages specific enzymatic reaction NTR hypoxic status, utilizing decreased NADH. The activity transforms nitroaromatic compounds into aromatic amines, which then reflected as measurable shift SERS signal probe. novel approach allows accurate quantification NTR, with sensitivity reaching detection threshold less than 0.02 μg/mL. probe's non-toxic nature superior biocompatibility facilitate use direct investigations A549 cells under reduced oxygen levels. We also applied this method xenograft model. results demonstrate marked increase levels tissues conditions, highlighting significance nanoprobe enhancing cancer diagnostics, helping medical doctors making decisions more swiftly effectively.

Язык: Английский

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