CMOS and CCD Detection in Raman Spectroscopy: A Comparison Using Spontaneous and Multiplex Coherent Anti‐Stokes Raman Scattering (CARS)
Journal of Raman Spectroscopy,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 28, 2025
ABSTRACT
Cooled
CCD
cameras
are
used
widely
in
spectroscopy,
mainly
due
to
their
sensitivity,
low
noise
under
low‐light
conditions,
and
relatively
high
image
spectral
readout
rates.
Despite
many
advantages,
have
limitations.
Particularly
bright‐light
such
as
those
encountered
with
coherent
Raman
spectroscopies,
the
finite
time
of
chips
becomes
limiting.
Furthermore,
when
weak
signals
need
be
observed
close
intense
signals,
blooming
smearing
limit
signal‐to‐noise
ratios
achievable.
Scientific
complementary
mixed
oxide
(sCMOS)–based
sensors
new.
Although
they
still
show
much
higher
than
cooled
CCDs,
application
spectroscopy
is
interest
given
rates
dynamic
ranges
possible.
Here,
we
evaluate
sCMOS
for
multiplex
(50
ps)
CARS
spontaneous
making
a
direct
comparison
state‐of‐the‐art
EM‐CCD
detector.
The
camera
outperforms
terms
limits
detection,
while
performs
better
range
rate.
Importantly,
an
does
not
suffer
from
interference
seen
cameras,
which
enables
observation
bands
(e.g.,
overtones)
more
signals.
that,
at
>
20–50
Hz,
performances
two
detector
types
substantially
different.
We
anticipate
that
sCMOS‐based
will
find
bright
CARS,
well
imaging.
Язык: Английский
Evaluating the single-shot photodegradation of optically-transparent bulk polymers under two-photon Raman-resonant laser irradiation in nanosecond regime (multiplex CARS test)
Polymer Testing,
Год журнала:
2024,
Номер
133, С. 108401 - 108401
Опубликована: Март 24, 2024
Язык: Английский
Imaging immunometabolism in situ in live animals
Immunometabolism,
Год журнала:
2024,
Номер
6(3), С. e00044 - e00044
Опубликована: Июль 1, 2024
Immunometabolism
is
a
rapidly
developing
field
that
holds
great
promise
for
diagnostic
and
therapeutic
benefits
to
human
diseases.
The
has
emerged
based
on
seminal
findings
from
in
vitro
ex
vivo
studies
established
the
fundamental
role
of
metabolism
immune
cell
effector
functions.
Currently,
acknowledging
necessity
investigating
cellular
within
natural
context
biological
processes.
Examining
cells
their
native
microenvironment
essential
not
only
reveal
cell-intrinsic
mechanisms
but
also
understand
how
cross-talk
between
neighboring
regulates
at
tissue
level
local
niche.
This
driving
innovation
advancement
multiple
imaging-based
technologies
enable
analysis
dynamic
intracellular
single-cell
level,
with
spatial
temporal
resolution.
In
this
review,
we
tally
currently
available
explore
emerging
methods
Raman
autofluorescence
lifetime
imaging
microscopy,
which
hold
significant
potential
offer
broad
applications
immunometabolism.
Язык: Английский