bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Дек. 2, 2022
Abstract
Somatic
cell
reprogramming
and
oncogenic
transformation
share
surprisingly
similar
features,
yet
transformed
cells
are
highly
resistant
to
reprogramming.
There
must
be
barriers
that
block
from
reprogramming,
but
the
nature
of
those
is
unclear.
In
this
study,
we
generated
a
systematic
panel
mouse
embryonic
fibroblasts
(MEFs)
using
variety
transgenes,
discovered
lines
remain
compatible
with
when
transfected
Oct4
/
Sox2
Klf4
Myc
.
By
comparing
reprogramming-capable
incapable
identified
multiple
stages
failure
in
process.
Some
failed
very
early,
whilst
other
seemed
progress
through
normal-looking
Finally,
show
MEK
inhibition
overcomes
one
critical
barrier
by
indirectly
suppressing
hyperactive
epigenetic
state
some
cells.
This
study
reveals
underlying
resistance
vary
between
different
methods.
Key
findings
context-specific
Inhibition
converts
Transformed
characterized
chromatin
affects
enable
Current Opinion in Obstetrics & Gynecology,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 24, 2025
Aneuploidy
is
a
major
cause
of
embryonic
arrest.
While
meiotic
aneuploidies,
especially
maternal,
are
well-documented
embryo
and
fetal
arrest,
increasing
evidence
highlights
the
crucial
role
played
by
mitotic
aneuploidies.
This
review
explores
molecular
cellular
pathways
underlying
these
abnormalities,
focusing
on
abnormal
cleavage,
chromatin
cohesion,
spindle
stability,
maternal
effect
genes,
mitochondria.
Approximately
half
human
embryos
cease
development
in
vitro
or
shortly
after
transfer
to
uterus.
Genetic
investigation
has
highlighted
that
90%
exhibit
Surprisingly,
most
arise
from
errors
during
early
divisions
preimplantation
embryos.
These
findings
strongly
correlate
with
disruptions
cleavage
possibly
due
faulty
assembly
mitochondrial
dysfunction
in-vitro
development.
Moreover,
effects,
such
as
recombination
variants
genes
involved
subcortical
complex,
may
further
predispose
high
rates
chromosomal
imbalance.
Meiotic
aneuploidies
play
significant
yet
their
origin
not
well
understood.
Investigating
lead
interventions
could
be
developed
improve
success
IVF
even
fertility
general.
Cell Reports,
Год журнала:
2023,
Номер
42(2), С. 112100 - 112100
Опубликована: Фев. 1, 2023
During
pre-implantation
stages
of
mammalian
development,
maternally
stored
material
promotes
both
the
erasure
sperm
and
oocyte
epigenetic
profiles
is
responsible
for
concomitant
genome
activation.
Here,
we
have
utilized
single-cell
methylome
transcriptome
sequencing
(scM&T-seq)
to
quantify
mRNA
expression
DNA
methylation
in
oocytes
a
developmental
series
human
embryos
at
resolution.
We
fully
characterize
embryonic
activation
maternal
transcript
degradation
map
key
reprogramming
events
developmentally
high-quality
embryos.
By
comparing
these
signatures
with
early
that
undergone
spontaneous
cleavage-stage
arrest,
as
determined
by
time-lapse
imaging,
identify
fail
appropriately
activate
their
genomes
or
undergo
reprogramming.
Our
results
indicate
failure
successfully
accomplish
essential
milestones
impedes
potential
likely
important
implications,
similar
aneuploidy,
success
assisted
reproductive
cycles.
Biology,
Год журнала:
2022,
Номер
11(8), С. 1121 - 1121
Опубликована: Июль 27, 2022
This
review
introduces
the
subject
of
senescence,
aging,
and
formation
senescent
multinucleated
giant
cells.
We
define
senescence
aging
describe
how
molecular
cellular
leads
to
organismal
senescence.
latest
information
on
cells’
phenotypes.
features
role
cells
in
aging-related
conditions
cancer.
explain
form
their
arteries
gonads.
also
reversibility
initiate
cancer
lead
progression
metastasis.
molecules
pathways
regulating
model
systems
applicability
clinical
therapies
age-related
diseases.
Antioxidants,
Год журнала:
2023,
Номер
12(12), С. 2117 - 2117
Опубликована: Дек. 14, 2023
Oocyte
quality
is
a
pivotal
determinant
of
assisted
reproductive
outcomes.
The
oocytes
intricately
linked
to
their
developmental
microenvironment,
particularly
the
levels
oxidative
stress
within
follicular
fluid.
Oxidative
in
fluid
may
have
substantial
influence
on
oocyte
health,
thereby
impacting
outcomes
ART
procedures.
This
review
meticulously
explores
intricate
relationship
between
and
Furthermore,
it
delves
into
strategies
aimed
at
ameliorating
status
fluid,
with
overarching
goal
enhancing
overall
efficacy
ART.
research
endeavors
establish
robust
foundation
provide
valuable
guidance
for
clinical
treatment
approaches,
context
infertile
women,
including
those
advanced
maternal
age.
Artificial Intelligence in Medicine,
Год журнала:
2024,
Номер
157, С. 102997 - 102997
Опубликована: Окт. 8, 2024
Nowadays,
the
most
adopted
technique
to
address
infertility
problems
is
in
vitro
fertilisation
(IVF).
However,
its
success
rate
limited,
and
associated
procedures,
known
as
assisted
reproduction
technology
(ART),
suffer
from
a
lack
of
objectivity
at
laboratory
level
clinical
practice.
This
paper
deals
with
applications
Artificial
Intelligence
(AI)
techniques
IVF
procedures.
intelligence
considered
promising
tool
for
ascertaining
quality
embryos,
critical
step
IVF.
Since
oocyte
influences
final
embryo
quality,
we
present
systematic
review
literature
on
AI-based
used
assess
quality;
analyse
results
discuss
several
research
directions.
In
particular,
highlight
how
can
support
process
examine
their
current
presented
literature.
Then,
challenges
must
face
fully
deploying
solutions
medical
Among
them,
availability
high-quality
data
sets
well
standardised
imaging
protocols
formats,
use
physics-informed
simulation
machine
learning
techniques,
study
informative,
descriptive
yet
observable
features,
and,
above
all,
studies
oocytes
specifically
about
live
birth
potential.
An
improved
understanding
determinants
improve
rates
while
reducing
costs,
risks
long-term
cultures,
bioethical
concerns.
