Viable SARS-CoV-2 Omicron sub-variants isolated from autopsy tissues

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Май 22, 2023

Pulmonary and extrapulmonary manifestations have been described after infection with SARS-CoV-2, the causative agent of coronavirus disease 2019 (COVID-19). The virus is known to persist in multiple organs due its tropism for several tissues. However, previous reports were unable provide definitive information about whether viable transmissible. It has hypothesized that persisting reservoirs SARS-CoV-2 tissues could be one potentially overlapping causes long COVID.In present study, we investigated autopsy materials obtained from 21 cadaveric donors documented first or reinfection at time death. cases studied included recipients different formulations COVID-19 vaccines. aim was find presence lungs, heart, liver, kidneys, intestines. We used two technical approaches: detection quantification viral genomic RNA using RT-qPCR, infectivity permissive vitro Vero E6 culture.All analyzed showed but dissimilar levels ranging 1.01 × 102 copies/mL 1.14 108 copies/mL, even among those who had vaccinated. Importantly, amounts replication-competent detected culture media highest load measured lung (≈1.4 106 copies/mL) heart (≈1.9 samples. Additionally, based on partial Spike gene sequences, characterization revealed Omicron sub-variants exhibiting a high level nucleotide amino acid identity them.These findings highlight can spread tissue locations such as intestines, both primary reinfections variant, contributing extending knowledge pathogenesis acute understanding sequelae clinical are observed during post-acute COVID-19.

Язык: Английский

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Potential long-term effects of SARS-CoV-2 infection on the pulmonary vasculature: Multilayered cross-talks in the setting of coinfections and comorbidities

PLoS Pathogens, Год журнала: 2023, Номер 19(1), С. e1011063 - e1011063

Опубликована: Янв. 12, 2023

The Coronavirus Disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) and its sublineages pose a new challenge to healthcare systems worldwide due ability efficiently spread in immunized populations resistance currently available therapies. COVID-19, although targeting primarily the respiratory system, is also now well established that later affects every organ body. Most importantly, despite therapy vaccine-elicited protection, long-term consequences of viral infection breakthrough asymptomatic individuals are areas concern. In past two years, investigators accumulated evidence on how virus triggers our immune system molecular signals involved cross-talk between cells structural pulmonary vasculature drive pathological lung complications such as endothelial dysfunction thrombosis. review, we emphasize recent updates pathophysiological inflammatory responses associated with SARS-CoV-2 their potential may consequently lead development vascular diseases.

Язык: Английский

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RNA: The most attractive target in recent viral diseases

Chemical Biology & Drug Design, Год журнала: 2023, Номер 103(1)

Опубликована: Дек. 13, 2023

Abstract As an expert in the field of drug design and discovery, I tried, this up‐to‐date perspective or commentary article, to recap shed light on previous latest revolutionary strategies employed medicinal therapeutic chemistry target principal viral weapon used by virulent RNA viruses (e.g., severe acute respiratory syndrome coronavirus 2 “SARS‐CoV‐2”) infect humans spread infections, genomic strands. These act taking advantage weakness points attractive bioweapon disable attack it (itself), accordingly stop entire reproduction, effectively end microbial infections such as disease 2019 (COVID‐19). The generation respective slightly falsely‐weaved strands, either endogenously exogenously, is key for designing most these approaches.

Язык: Английский

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Understanding virus–host interactions in tissues

Nature Microbiology, Год журнала: 2023, Номер 8(8), С. 1397 - 1407

Опубликована: Июль 24, 2023

Язык: Английский

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Age-associated features of norovirus infection analysed in mice

Nature Microbiology, Год журнала: 2023, Номер 8(6), С. 1095 - 1107

Опубликована: Май 15, 2023

Язык: Английский

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