Actin Cytoskeleton at the Synapse: An Alzheimer's Disease Perspective

Cytoskeleton, Год журнала: 2025, Номер unknown

Опубликована: Янв. 22, 2025

ABSTRACT Actin, a ubiquitous and highly conserved cytoskeletal protein, plays pivotal role in various cellular functions such as structural support, facilitating cell motility, contributing to the dynamic processes of synaptic function. Apart from its established inducing morphological changes, recent developments field indicate an active involvement actin modulating both structure function pre‐ postsynaptic terminals. Within presynapse, it is involved organization trafficking vesicles, neurotransmitter release. In postsynapse, dynamically modulates dendritic spines, influencing density anchoring receptors. addition, interplay at synapse underscores essential regulating neural communication. This review strives offer comprehensive overview advancements understanding multifaceted cytoskeleton functions. By emphasizing aberrant regulation, we aim provide valuable insights into underlying mechanisms Alzheimer's disease pathophysiology.

Язык: Английский

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Intracellular protein-lipid interactions drive presynaptic assembly prior to neurexin recruitment

Neuron, Год журнала: 2025, Номер 113(5), С. 737 - 753.e6

Опубликована: Янв. 14, 2025

Язык: Английский

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A Chemogenetic Toolkit for Inducible, Cell Type‐Specific Actin Disassembly

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

The actin cytoskeleton and its nanoscale organization are central to all eukaryotic cells-powering diverse cellular functions including morphology, motility, cell division-and is dysregulated in multiple diseases. Historically studied largely with purified proteins or isolated cells, tools study type-specific roles of multicellular contexts greatly needed. DeActs recently created, first-in-class genetic for perturbing nanostructures dynamics specific types across model organisms. Here, ChiActs introduced, the next generation actin-perturbing that can be rapidly activated cells optogenetically targeted distinct subcellular locations using light. composed split halves DeAct-SpvB, whose potent disassembly-promoting activity restored by chemical-induced dimerization allosteric switching. It shown function induce disassembly several able perturb actin-dependent nano-assembly functions, inhibiting lamellipodial protrusions membrane ruffling, remodeling mitochondrial reorganizing chromatin locally constraining compartments. thus expand toolbox genetically-encoded living unlocking studies many complex systems.

Язык: Английский

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Neurexin drives Caenorhabditis elegans avoidance behavior independently of its post-synaptic binding partner neuroligin

G3 Genes Genomes Genetics, Год журнала: 2024, Номер 14(8)

Опубликована: Май 23, 2024

Abstract Neurexins and their canonical binding partners, neuroligins, are localized to neuronal pre-, post-synapses, respectively, but less is known about role in driving behaviors. Here, we use the nematode C. elegans show that neurexin, not neuroligin, required for avoiding specific chemorepellents. We find adults with knockouts of entire neurexin locus exhibit a strong avoidance deficit response glycerol weaker defect copper. Notably, (nrx-1) locus, like its mammalian homologs, encodes multiple isoforms, α γ. Using isoform-specific mutations, γ isoform selectively avoidance. Next, used transgenic rescue experiments this functions at least partially nervous system. also confirm transgenes expressed neurons observe protein accumulation neurites. Furthermore, tested whether these mutants affect behavioral responses juveniles. juveniles (4th larval stages) knocking out or α-isoforms, γ-isoform, defective glycerol. These results suggest different isoforms chemosensory behavior adults, providing general principle how conserved gene across species.

Язык: Английский

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Autophagy in the retina affects photoreceptor synaptic plasticity and behavior

Journal of Insect Physiology, Год журнала: 2024, Номер 161, С. 104741 - 104741

Опубликована: Дек. 10, 2024

Язык: Английский

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Targeting the intracellular neurexin interactome by in vivo proximity ligation

Trends in Neurosciences, Год журнала: 2024, Номер 47(4), С. 243 - 245

Опубликована: Март 6, 2024

Язык: Английский

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