An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing

Nature Communications, Год журнала: 2018, Номер 9(1)

Опубликована: Окт. 8, 2018

Abstract Through analysis of the Drosophila ionotropic receptors (IRs), a family variant glutamate receptors, we reveal that most IRs are expressed in peripheral neuron populations diverse gustatory organs larvae and adults. We characterise IR56d, which defines two anatomically-distinct classes proboscis: one responds to carbonated solutions fatty acids while other represents subset sugar- acid-sensing cells. Mutational indicates together with broadly-expressed co-receptors IR25a IR76b, is essential for physiological responses carbonation acids, but not sugars. further demonstrate both promote IR56d-dependent attraction flies, through different behavioural outputs. Our work provides toolkit investigating taste functions IRs, these required sensing, illustrates how system uses combinatorial expression sensory molecules distinct neurons coordinate behaviour.

Язык: Английский

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Recent advances in the genetic basis of taste detection in Drosophila

Cellular and Molecular Life Sciences, Год журнала: 2019, Номер 77(6), С. 1087 - 1101

Опубликована: Окт. 9, 2019

Язык: Английский

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Drosophilasensory receptors—a set of molecular Swiss Army Knives

Genetics, Год журнала: 2021, Номер 217(1), С. 1 - 34

Опубликована: Янв. 1, 2021

Genetic approaches in the fruit fly, Drosophila melanogaster, have led to a major triumph field of sensory biology-the discovery multiple large families receptors and channels. Some these families, such as transient receptor potential channels, are conserved from animals ranging worms humans, while others, "gustatory receptors," "olfactory "ionotropic restricted invertebrates. Prior identification flies, it was widely assumed that proteins function just one modality vision, smell, taste, hearing, somatosensation, which includes thermosensation, light, noxious mechanical touch. By employing vast combination genetic, behavioral, electrophysiological, other concept emerge is many multitaskers. The earliest example this idea individual channels senses. It now clear multitasking exhibited by including gustatory receptors, ionotropic epithelial Na+ (also referred Pickpockets), even opsins, were formerly thought exclusively light sensors. characterizations neurons express them also reveal mechanisms through flies can accurately differentiate between different stimuli when they activate same receptor, well adaptation, amplification, integration. insights gleaned studies been highly influential directing investigations animal models.

Язык: Английский

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Mechanisms of lactic acid gustatory attraction in Drosophila

Current Biology, Год журнала: 2021, Номер 31(16), С. 3525 - 3537.e6

Опубликована: Июнь 30, 2021

Язык: Английский

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Ir56d-dependent fatty acid responses in Drosophila uncover taste discrimination between different classes of fatty acids

eLife, Год журнала: 2021, Номер 10

Опубликована: Май 5, 2021

Chemosensory systems are critical for evaluating the caloric value and potential toxicity of food. While animals can discriminate between thousands odors, much less is known about discriminative capabilities taste systems. Fats sugars represent calorically potent attractive food sources that contribute to hedonic feeding. Despite differences in nutritional fats sugars, ability system different rewarding tastants thought be limited. In Drosophila, neurons expressing ionotropic receptor 56d (IR56d) required reflexive behavioral responses medium-chain fatty acid, hexanoic acid. Here, we tested whether flies classes acids using an aversive memory assay. Our results indicate able from both short- long-chain acids, but not other acids. IR56d broadly responsive short-, medium-, genetic deletion selectively disrupts response Further, IR56d+ GR64f+ necessary proboscis extension (PER) GR64f dispensable PER indicating involvement one or more neurons. Together, these findings reveal acid taste, discrimination occurs through differential activation shared populations study uncovers a capacity encode tastant identity within category.

Язык: Английский

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Molecular sensors in the taste system of Drosophila

Genes & Genomics, Год журнала: 2023, Номер 45(6), С. 693 - 707

Опубликована: Фев. 24, 2023

Язык: Английский

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Molecular Basis of Hexanoic Acid Taste in Drosophila melanogaster

Molecules and Cells, Год журнала: 2023, Номер 46(7), С. 451 - 460

Опубликована: Май 19, 2023

Animals generally prefer nutrients and avoid toxic harmful chemicals.Recent behavioral physiological studies have identified that sweet-sensing gustatory receptor neurons (GRNs) in Drosophila melanogaster mediate appetitive behaviors toward fatty acids.Sweet-sensing GRN activation requires the function of ionotropic receptors IR25a, IR56d, IR76b, as well GR64e.However, we reveal hexanoic acid (HA) is rather than nutritious to D. melanogaster.HA one major components fruit Morinda citrifolia (noni).Thus, analyzed responses noni acids, HA, via electrophysiology proboscis extension response (PER) assay.Electrophysiological tests show this reminiscent arginine-mediated neuronal responses.Here, determined a low concentration HA induced attraction, which was mediated by GRNs, high aversion, bitter-sensing GRNs.We also demonstrated elicits attraction mainly GR64d IR56d expressed but activates three (GR32a, GR33a, GR66a) GRNs.The mechanism sensing biphasic dose dependent manner.Furthermore, inhibit sugar-mediated like other bitter compounds.Taken together, discovered binary HA-sensing may be evolutionarily meaningful foraging niche insects.

Язык: Английский

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A systematic review of the biological mediators of fat taste and smell

Physiological Reviews, Год журнала: 2022, Номер 103(1), С. 855 - 918

Опубликована: Сен. 15, 2022

Taste and smell play a key role in our ability to perceive foods. Overconsumption of highly palatable energy-dense foods can lead increased caloric intake obesity. Thus there is growing interest the study biological mediators fat taste associated olfaction as potential targets for pharmacologic nutritional interventions context obesity health. The number studies examining mechanisms underlying has grown rapidly last 5 years. Therefore, purpose this systematic review summarize emerging evidence smell. A literature search was conducted published English between 2014 2021 adult humans animal models. Database searches were using PubMed, EMBASE, Scopus, Web Science terms including fat/lipid, taste, olfaction. Initially, 4,062 articles identified through database searches, total 84 relevant met inclusion exclusion criteria are included review. Existing suggests that several proteins integral chemosensation, cluster differentiation 36 (CD36) G protein-coupled receptor 120 (GPR120). This will discuss these signal transduction pathways involved detection. We also neural circuits, brain regions, ingestive cues, postingestive signals, genetic polymorphism perception consumption. Finally, we eating behavior

Язык: Английский

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A single pair of pharyngeal neurons functions as a commander to reject high salt in Drosophila melanogaster

eLife, Год журнала: 2023, Номер 12

Опубликована: Дек. 27, 2023

Salt (NaCl), is an essential nutrient for survival, while excessive salt can be detrimental. In the fruit fly, Drosophila melanogaster , internal taste organs in pharynx are critical gatekeepers impacting decision to accept or reject a food. Currently, our understanding of mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high rudimentary. Here, we found that member ionotropic family, Ir60b expressed exclusively pair GRNs activated by salt. Using two-way choice assay (DrosoX) measure ingestion volume, demonstrate IR60b and two co-receptors IR25a IR76b required prevent consumption. Mutants lacking external but retaining exhibit much higher avoidance than flies with all missing three IRs. Our findings highlight vital role IRs GRN control

Язык: Английский

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The taste of vitamin C in Drosophila

EMBO Reports, Год журнала: 2023, Номер 24(6)

Опубликована: Апрель 28, 2023

Язык: Английский

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