Biological Chemistry,
Год журнала:
2023,
Номер
404(8-9), С. 769 - 779
Опубликована: Июнь 28, 2023
Mitochondria
are
the
essential
players
in
eukaryotic
ATP
production
by
oxidative
phosphorylation,
which
relies
on
maintenance
and
accurate
expression
of
mitochondrial
genome.
Even
though
basic
principles
translation
conserved
due
to
descendance
from
a
bacterial
ancestor,
some
deviations
regarding
factors
as
well
mRNA
characteristics
applied
genetic
code
present
human
mitochondria.
Together,
these
features
certain
challenges
during
mitochondrion
has
handle.
Here,
we
discuss
current
knowledge
focusing
termination
process
associated
quality
control
mechanisms.
We
describe
how
mtRF1a
resembles
RF1
mechanistically
summarize
vitro
recent
vivo
data
leading
conclusion
being
major
release
factor.
On
other
hand,
ongoing
debate
about
function
second
codon-dependent
factor
mtRF1
its
role
specialized
Finally,
link
defects
activation
rescue
mechanisms
highlighting
importance
ribosome-associated
for
sufficient
respiratory
therefore
health.
The
human
mitochondrial
genome
encodes
crucial
oxidative
phosphorylation
system
proteins,
pivotal
for
aerobic
energy
transduction.
They
are
translated
from
nine
monocistronic
and
two
bicistronic
transcripts
whose
native
structures
remain
unexplored,
posing
a
gap
in
understanding
gene
expression.
In
this
work,
we
devised
the
dimethyl
sulfate
mutational
profiling
with
sequencing
(mitoDMS-MaPseq)
method
applied
detection
of
RNA
folding
ensembles
using
expectation-maximization
(DREEM)
clustering
to
unravel
messenger
(mt-mRNA)
structurome
wild-type
(WT)
leucine-rich
pentatricopeptide
repeat-containing
protein
(LRPPRC)-deficient
cells.
Our
findings
elucidate
LRPPRC's
role
as
holdase
contributing
maintaining
mt-mRNA
efficient
translation.
structural
insights
WT
mitochondria,
coupled
metabolic
labeling,
unveil
potential
mRNA-programmed
translational
pausing
distinct
programmed
ribosomal
frameshifting
mechanism.
data
define
critical
layer
expression
regulation.
These
maps
provide
reference
studying
diverse
physiological
pathological
contexts.
Journal of Chemistry,
Год журнала:
2022,
Номер
2022, С. 1 - 19
Опубликована: Май 17, 2022
Cancer
is
characterized
by
abnormal
cell
differentiation
in
or
on
the
part
of
body.
The
most
commonly
used
chemotherapeutic
drugs
are
developed
to
target
rapidly
dividing
cells,
such
as
cancer
but
they
also
damage
healthy
epithelial
cells.
This
has
serious
consequences
for
normal
cells
and
become
responsible
development
various
disorders.
Several
strategies
delivering
cytotoxic
cancerous
sites
that
limit
systemic
toxicity
other
adverse
effects
have
recently
been
evolved.
Among
them,
biomolecule-conjugated
metal
complexes-based
targeting
shown
tremendous
advantages
therapy.
review
focuses
several
chemoselective
biomolecules-bound
complexes
prospective
therapy-targeted
agents.
In
this
review,
we
presented
details
extra-
intracellular
mechanisms
We
addressed
current
clinical
issues
recent
therapeutic
targeted
therapy
may
pave
a
way
future
direction
Molecular Cell,
Год журнала:
2022,
Номер
82(19), С. 3646 - 3660.e9
Опубликована: Авг. 30, 2022
The
human
mitochondrial
genome
must
be
replicated
and
expressed
in
a
timely
manner
to
maintain
energy
metabolism
supply
cells
with
adequate
levels
of
adenosine
triphosphate.
Central
this
process
is
the
idea
that
replication
primers
gene
products
both
arise
via
transcription
from
single
light
strand
promoter
(LSP)
such
primer
formation
can
influence
expression,
no
consensus
as
how
regulated.
Here,
we
report
discovery
second
(LSP2)
humans,
features
characteristic
bona
fide
promoter.
We
propose
position
LSP2
on
allows
expression
orchestrated
two
distinct
sites,
which
expands
our
long-held
understanding
humans.
Journal of Oral Pathology and Medicine,
Год журнала:
2023,
Номер
52(8), С. 738 - 745
Опубликована: Авг. 3, 2023
Abstract
Background
Transforming
growth
factor
β
regulator
4
(TBRG4)
is
a
potential
prognostic
indicator
in
various
cancers,
especially
squamous
cell
carcinomas,
and
associated
with
disease
amelioration
poor
outcomes.
The
study
aimed
to
assess
the
expression
pattern
of
TBRG4
patients
operable
oral
carcinoma
(OSCC)
understand
its
role
tumour
progression
using
indicators
outcome
like
stage,
grade,
nodal
metastasis,
invasion.
Methods
was
assessed
by
analyzing
51
cancer
adjacent
non‐cancerous
tissues
OSCC
quantitative
real‐time
PCR,
Western
blot.
also
analysed
Cancer
Genome
Atlas
Head–Neck
Squamous
Cell
Carcinoma
(TCGA‐HNSC)
dataset
UALCAN
tool
(
http://ualcan.path.uab.edu/
).
relationship
between
patient's
prognosis
Kaplan–Meier
plotter.
Results
Both
mRNA
protein
levels
were
significantly
increased
tissues.
TBGR4
advanced
stages
(III
IV)
worst
invasion
(WPOI‐4
5).
High
reduced
overall
survival
p
=
0.011).
In
addition,
analysis
gene
clinical
data
from
TCGA,
identified
that
highly
expressed
HPV
negative
positively
correlated
survival.
Conclusion
present
suggests
high
might
serve
as
novel
biomarker
for
HPV‐negative
OSCC,
which
can
be
validated
future
additional
investigations
larger
cohorts
along
functional
studies.
Human Molecular Genetics,
Год журнала:
2024,
Номер
33(R1), С. R19 - R25
Опубликована: Май 22, 2024
Human
mitochondria
harbour
a
circular,
polyploid
genome
(mtDNA)
encoding
11
messenger
RNAs
(mRNAs),
two
ribosomal
(rRNAs)
and
22
transfer
(tRNAs).
Mitochondrial
transcription
produces
long,
polycistronic
transcripts
that
span
almost
the
entire
length
of
genome,
hence
contain
all
three
types
RNAs.
The
primary
then
undergo
number
processing
maturation
steps,
which
constitute
key
regulatory
points
mitochondrial
gene
expression.
first
step
RNA
consists
separation
into
individual,
functional
molecules
can
occur
by
distinct
pathways.
Both
are
carried
out
dedicated
molecular
machineries
substantially
differ
from
enzymes
found
elsewhere.
As
result,
underlying
mechanisms
remain
poorly
understood.
Over
last
years,
genetic,
biochemical
structural
studies
have
identified
players
involved
in
both
pathways
provided
insights
mechanisms.
Here,
we
review
our
current
understanding
mammalian
provide
an
outlook
on
open
questions
field.
