Juvenile
hormone
(JH)
is
important
to
maintain
insect
larval
status;
however,
its
cell
membrane
receptor
has
not
been
identified.
Using
the
lepidopteran
Helicoverpa
armigera
(cotton
bollworm),
a
serious
agricultural
pest,
as
model,
we
determined
that
tyrosine
kinases
(RTKs)
cadherin
96ca
(CAD96CA)
and
fibroblast
growth
factor
homologue
(FGFR1)
function
JH
receptors
by
their
roles
in
JH-regulated
gene
expression,
status
maintaining,
rapid
intracellular
calcium
increase,
phosphorylation
of
MET1
cofactor
Taiman,
high
affinity
III.
Gene
knockout
Cad96ca
Fgfr1
CRISPR/Cas9
embryo
knockdown
various
cells,
overexpression
CAD96CA
FGFR1
mammalian
HEK-293T
cells
all
supported
transmitting
signal
receptors.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2021,
Номер
unknown
Опубликована: Окт. 1, 2021
Summary
Germ
cells
are
essential
to
sexual
reproduction.
Across
the
animal
kingdom,
extracellular
signaling
isoprenoids,
such
as
retinoic
acids
(RAs)
in
vertebrates
and
juvenile
hormones
(JHs)
invertebrates,
facilitate
multiple
processes
germline
lifecycle.
Here
we
investigated
role
of
these
potent
molecules
embryonic
germ
cell
migration,
using
JHs
Drosophila
melanogaster
a
model
system.
In
contrast
their
established
endocrine
roles
during
larval
adult
development,
found
that
JH
acts
locally
development.
Using
an
vivo
biosensor,
is
first
active
near
primordial
(PGCs)
they
migrate
developing
somatic
gonad.
Through
vitro
assays,
both
necessary
sufficient
for
PGC
migration.
Analysis
into
mechanisms
this
newly
uncovered
paracrine
function
revealed
migration
was
compromised
when
were
reduced
or
increased,
suggesting
specific
titers
spatiotemporal
dynamics
required
robust
colonization
Compromised
can
impair
fertility
cause
tumors
many
species,
including
humans.
mammals,
retinoids,
JH-related
family
has
development
We
acid,
like
JH,
impact
.
Together,
our
study
reveals
previously
unanticipated
isoprenoids
local
effectors
pre-gonadal
suggests
broadly
shared
mechanism
Highlights
Juvenile
(JH)
&
Primordial
Cell
(PGC)
directly
around
migrating
PGCs
prior
its
function.
Compensatory
feedback
sensitive
receptor
maintains
homeostasis.
JH-like
may
have
similar
mammalian
Juvenile
hormone
(JH)
is
important
to
maintain
insect
larval
status;
however,
its
cell
membrane
receptor
has
not
been
identified.
Using
the
lepidopteran
Helicoverpa
armigera
(cotton
bollworm),
a
serious
agricultural
pest,
as
model,
we
determined
that
tyrosine
kinases
(RTKs)
cadherin
96ca
(CAD96CA)
and
fibroblast
growth
factor
homologue
(FGFR1)
function
JH
receptors
by
their
roles
in
JH-regulated
gene
expression,
status
maintaining,
calcium
increase,
phosphorylation
of
intracellular
MET1
cofactor
Taiman,
high
affinity
III.
Gene
knockout
Cad96ca
Fgfr1
CRISPR/Cas9
embryo
knockdown
various
cells,
overexpression
CAD96CA
FGFR1
mammalian
HEK-293T
cells
all
supported
transmitting
signal
receptors.
Egyptian Academic Journal of Biological Sciences,
Год журнала:
2023,
Номер
16(2), С. 115 - 124
Опубликована: Июнь 20, 2023
Laboratory
experiments
evaluated
the
latent
effects
of
lufenuron
and
rice
bran
extract
on
newly
hatched
nymphs
Schistocerca
gregaria
produced
from
females
who
were
treated
as
fifth
nymphal
instar
with
LC50
each
extract.Semithin
sections
hatchling
showed
great
histological
malformations
in
structure
brain,
compound
eyes,
midgut,
hind
leg
muscles
compared
control,
these
organs
lost
their
normal
structures,
shapes,
functions.The
tested
compounds
had
serious
extended
histopathological
effects,
so
they
can
be
used
to
control
S.
safe
alternative
agents
toxic
synthetic
pesticides.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 28, 2024
Abstract
Juvenile
hormone
(JH)
is
important
to
maintain
insect
larval
status;
however,
its
cell
membrane
receptor
has
not
been
identified.
Using
the
lepidopteran
Helicoverpa
armigera
(cotton
bollworm),
a
serious
agricultural
pest,
as
model,
we
determined
that
tyrosine
kinases
(RTKs)
cadherin
96ca
(CAD96CA)
and
fibroblast
growth
factor
homologue
(FGFR1)
function
JH
receptors
by
their
roles
in
JH-regulated
gene
expression,
status
maintaining,
rapid
intracellular
calcium
increase,
phosphorylation
of
MET1
cofactor
Taiman,
high
affinity
III.
Gene
knockout
Cad96ca
Fgfr1
CRISPR/Cas9
embryo
knockdown
various
cells,
overexpression
CAD96CA
FGFR1
mammalian
HEK-293T
cells
all
supported
transmitting
signal
receptors.
Juvenile
hormone
(JH)
is
important
to
maintain
insect
larval
status;
however,
its
cell
membrane
receptor
has
not
been
identified.
Using
the
lepidopteran
Helicoverpa
armigera
(cotton
bollworm),
a
serious
agricultural
pest,
as
model,
we
determined
that
tyrosine
kinases
(RTKs)
cadherin
96ca
(CAD96CA)
and
fibroblast
growth
factor
homologue
(FGFR1)
function
JH
receptors
by
their
roles
in
JH-regulated
gene
expression,
status
maintaining,
rapid
intracellular
calcium
increase,
phosphorylation
of
MET1
cofactor
Taiman,
high
affinity
III.
Gene
knockout
Cad96ca
Fgfr1
CRISPR/Cas9
embryo
knockdown
various
cells,
overexpression
CAD96CA
FGFR1
mammalian
HEK-293T
cells
all
supported
transmitting
signal
receptors.
