Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone DOI Creative Commons
Yan-Xue Li, Xin-Le Kang, Yan-Li Li

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Май 16, 2024

Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.

Язык: Английский

Juvenile hormones direct primordial germ cell migration to the embryonic gonad DOI Open Access
Lacy J. Barton,

Sanny Justina,

Emily P. Dawson

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2021, Номер unknown

Опубликована: Окт. 1, 2021

Summary Germ cells are essential to sexual reproduction. Across the animal kingdom, extracellular signaling isoprenoids, such as retinoic acids (RAs) in vertebrates and juvenile hormones (JHs) invertebrates, facilitate multiple processes germline lifecycle. Here we investigated role of these potent molecules embryonic germ cell migration, using JHs Drosophila melanogaster a model system. In contrast their established endocrine roles during larval adult development, found that JH acts locally development. Using an vivo biosensor, is first active near primordial (PGCs) they migrate developing somatic gonad. Through vitro assays, both necessary sufficient for PGC migration. Analysis into mechanisms this newly uncovered paracrine function revealed migration was compromised when were reduced or increased, suggesting specific titers spatiotemporal dynamics required robust colonization Compromised can impair fertility cause tumors many species, including humans. mammals, retinoids, JH-related family has development We acid, like JH, impact . Together, our study reveals previously unanticipated isoprenoids local effectors pre-gonadal suggests broadly shared mechanism Highlights Juvenile (JH) & Primordial Cell (PGC) directly around migrating PGCs prior its function. Compensatory feedback sensitive receptor maintains homeostasis. JH-like may have similar mammalian

Язык: Английский

Процитировано

4

Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone DOI Open Access
Yan-Xue Li, Xin-Le Kang, Yan-Li Li

и другие.

Опубликована: Май 16, 2024

Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, calcium increase, phosphorylation of intracellular MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.

Язык: Английский

Процитировано

0

Latent Histopathological Effects of Lufenuron and Rice Bran Extract on Schistocerca Gregaria (Forskal) (Orthoptera: Acrididae) DOI Creative Commons

Noura Mahdy,

Samira Abd-El Wahed,

Amira Abdel hamid

и другие.

Egyptian Academic Journal of Biological Sciences, Год журнала: 2023, Номер 16(2), С. 115 - 124

Опубликована: Июнь 20, 2023

Laboratory experiments evaluated the latent effects of lufenuron and rice bran extract on newly hatched nymphs Schistocerca gregaria produced from females who were treated as fifth nymphal instar with LC50 each extract.Semithin sections hatchling showed great histological malformations in structure brain, compound eyes, midgut, hind leg muscles compared control, these organs lost their normal structures, shapes, functions.The tested compounds had serious extended histopathological effects, so they can be used to control S. safe alternative agents toxic synthetic pesticides.

Язык: Английский

Процитировано

1

Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone DOI Creative Commons
Yan-Xue Li, Xin-Le Kang, Yan-Li Li

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 28, 2024

Abstract Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.

Язык: Английский

Процитировано

0

Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone DOI Creative Commons
Yan-Xue Li, Xin-Le Kang, Yan-Li Li

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Май 16, 2024

Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as model, we determined that tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor homologue (FGFR1) function JH receptors by their roles in JH-regulated gene expression, status maintaining, rapid intracellular calcium increase, phosphorylation of MET1 cofactor Taiman, high affinity III. Gene knockout Cad96ca Fgfr1 CRISPR/Cas9 embryo knockdown various cells, overexpression CAD96CA FGFR1 mammalian HEK-293T cells all supported transmitting signal receptors.

Язык: Английский

Процитировано

0