Helper NLR immune protein NRC3 evolved to evade inhibition by a cyst nematode virulence effector DOI Creative Commons
Yu Sugihara, Jiorgos Kourelis, Mauricio P. Contreras

и другие.

PLoS Genetics, Год журнала: 2025, Номер 21(4), С. e1011653 - e1011653

Опубликована: Апрель 9, 2025

Parasites can counteract host immunity by suppressing nucleotide binding and leucine-rich repeat (NLR) proteins that function as immune receptors. We previously showed a cyst nematode virulence effector SPRYSEC15 (SS15) binds inhibits oligomerisation of helper NLR in the expanded NRC1/2/3 clade preventing intramolecular rearrangements required for NRC into an activated resistosome. Here we examined degree to which from multiple Solanaceae species are sensitive suppression SS15 tested hypotheses about adaptive evolution interface between inhibitor proteins. Whereas all orthologs NRC2 were inhibited SS15, some natural variants NRC1 NRC3 insensitive suppression. Ancestral sequence reconstruction combined with functional assays revealed transitioned ancestral suppressed form one over 19 million years ago. Our analyses evolutionary trajectory receptor against parasite inhibitor, identifying key transitions NLRs this inhibition. This work reveals distinct type gene-for-gene interaction or pathogen immunosuppressors receptors contrasts coevolution AVR effectors

Язык: Английский

Helper NLR immune protein NRC3 evolved to evade inhibition by a cyst nematode virulence effector DOI Creative Commons
Yu Sugihara, Jiorgos Kourelis, Mauricio P. Contreras

и другие.

PLoS Genetics, Год журнала: 2025, Номер 21(4), С. e1011653 - e1011653

Опубликована: Апрель 9, 2025

Parasites can counteract host immunity by suppressing nucleotide binding and leucine-rich repeat (NLR) proteins that function as immune receptors. We previously showed a cyst nematode virulence effector SPRYSEC15 (SS15) binds inhibits oligomerisation of helper NLR in the expanded NRC1/2/3 clade preventing intramolecular rearrangements required for NRC into an activated resistosome. Here we examined degree to which from multiple Solanaceae species are sensitive suppression SS15 tested hypotheses about adaptive evolution interface between inhibitor proteins. Whereas all orthologs NRC2 were inhibited SS15, some natural variants NRC1 NRC3 insensitive suppression. Ancestral sequence reconstruction combined with functional assays revealed transitioned ancestral suppressed form one over 19 million years ago. Our analyses evolutionary trajectory receptor against parasite inhibitor, identifying key transitions NLRs this inhibition. This work reveals distinct type gene-for-gene interaction or pathogen immunosuppressors receptors contrasts coevolution AVR effectors

Язык: Английский

Процитировано

0