How to enjoy laccases

Cellular and Molecular Life Sciences, Год журнала: 2015, Номер 72(5), С. 923 - 940

Опубликована: Янв. 10, 2015

Язык: Английский

---

Aptitude of Oxidative Enzymes for Treatment of Wastewater Pollutants: A Laccase Perspective

Molecules, Год журнала: 2019, Номер 24(11), С. 2064 - 2064

Опубликована: Май 30, 2019

Natural water sources are very often contaminated by municipal wastewater discharges which contain either of xenobiotic pollutants and their sometimes more toxic degradation products, or both, frustrates the universal millenium development goal provision relatively scarce pristine freshwater to water-scarce -stressed communities, in order augment socioeconomic well-being. Seeing that both regulatory measures, as regards discharge limits wastewater, query for efficient treatment methods remain unanswered, partially, prospects enzymatic is advisable. Therefore, a reconsideration was assigned possible capacity oxidative enzymes respective challenges encountered during applications treatment, ultimately, laccase, polyphenol oxidase oxidizes aromatic inorganic substrates with electron-donating groups contaminants real situations, since it assumed be vehicle greener community. Furthermore, importance laccase-driven catalysis toward maintaining mass-energy balance, hence minimizing environmental waste, comprehensibly elucidated, well strategic positioning laccase model facility effective contaminants.

Язык: Английский

---

Biochemical properties and yields of diverse bacterial laccase-like multicopper oxidases expressed in Escherichia coli

Scientific Reports, Год журнала: 2015, Номер 5(1)

Опубликована: Июнь 12, 2015

Laccases are multi-copper oxidases that oxidize a broad range of substrates at the expense molecular oxygen, without any need for co-factor regeneration. These enzymes bear high potential sustainable synthesis fine chemicals and modification (bio)polymers. Here we describe cloning expression five novel bacterial laccase-like multi copper (LMCOs) diverse origin which were identified by homology searches in online databases. Activity yields under different conditions temperature stabilities compared to three previously described from Bacillus subtilis, pumilus clausii. In almost all cases, switch oxygen-limited growth after induction increased volumetric activity considerably. For proteins with predicted signal peptides secretion, recombinant sequence was investigated. CotA-type LMCOs outperformed Streptomyces Gram-negative bacteria respect Escherichia coli application relevant biochemical properties. The coagulans LMCO combined E. unprecedented strong alkaline pH storage stability, making it promising candidate further development.

Язык: Английский

---

Applications of Microbial Laccases: Patent Review of the Past Decade (2009–2019)

Catalysts, Год журнала: 2019, Номер 9(12), С. 1023 - 1023

Опубликована: Дек. 4, 2019

There is a high number of well characterized, commercially available laccases with different redox potentials and low substrate specificity, which in turn makes them attractive for vast array biotechnological applications. Laccases operate as batteries, storing electrons from individual oxidation reactions to reduce molecular oxygen, releasing water the only by-product. Due society’s increasing environmental awareness global intensification bio-based economies, industry also expanding. Enzymes such are seen better alternative use wood, paper, textile, food industries, they being applied biocatalysts, biosensors, biofuel cells. Almost 140 years first description laccase, industrial implementations these enzymes still remain scarce comparison their potential, mostly due production costs limited control enzymatic reaction side product(s). This review summarizes laccase applications last decade, focusing on published patents during this period.

Язык: Английский

---

Laccases as versatile enzymes: from industrial uses to novel applications

Journal of Chemical Technology & Biotechnology, Год журнала: 2019, Номер 95(3), С. 481 - 494

Опубликована: Окт. 6, 2019

Abstract The application of enzymes offers an enormous potential in the improvement existing industrial procedures and establishment new processes for obtaining high‐added value products. Enzymes provide cleaner more efficient contribute to sustainability concept. In this sense, laccases are very versatile biocatalysts currently used food, textile pulp paper sectors among others. During last years, scientific efforts have been diverted exploitation such interesting novel fields like lignocellulosic biorefineries, biosensors or enzymatic biofuel cells. This review provides a general vision use laccase describing their main characteristics mode action. Furthermore, current uses summarized most revealed. increasing interest on is also demonstrated by research enzyme engineering as it detailed review. © 2019 Society Chemical Industry

Язык: Английский

---

Biotransformation of Plant-Derived Phenolic Acids

Biotechnology Journal, Год журнала: 2017, Номер unknown, С. 1700632 - 1700632

Опубликована: Дек. 26, 2017

Phenolic acids are abundant biomass feedstock that can be derived from the processing of lignin or other byproducts agro-industrial waste. Although phenolic such as p-hydroxybenzoic acid, p-coumaric caffeic vanillic cinnamic gallic syringic and ferulic acid used directly in various applications, their value significantly increased when they further modified to high value-added compounds. This review summarizes discusses new advances cell-free whole-cell biocatalysis technologies for reactions important conversion including esterification, decarboxylation, amination, halogenation, hydroxylation, ring-breakage reactions. The products these useful pharmaceutical, cosmetic, food, fragrance, polymer industries. Production is sustainable, processes biotransformation clean do not produce toxic waste use less energy than conventional physical chemical methods. Thus, provides an economically viable sustainable means producing materials society.

Язык: Английский

---

Functional Enzyme Mimics for Oxidative Halogenation Reactions that Combat Biofilm Formation

Advanced Materials, Год журнала: 2018, Номер 30(36)

Опубликована: Июнь 19, 2018

Abstract Transition‐metal oxide nanoparticles and molecular coordination compounds are highlighted as functional mimics of halogenating enzymes. These enzymes involved in halometabolite biosynthesis. Their activity is based upon the formation hypohalous acids from halides hydrogen peroxide or oxygen, which form bioactive secondary metabolites microbial origin with strong antibacterial antifungal activities follow‐up reactions. Therefore, enzyme may be valuable tools to combat biofilm formation. Here, models briefly described, classified according their catalytic functions, current knowledge about settlement chemistry adhesion fouling organisms summarized. Enzyme highest potential showcased. They find application antifouling coatings, indoor outdoor paints, polymer membranes for water desalination, aquacultures, but also on surfaces food packaging, door handles, hand rails, push buttons, keyboards, other elements made plastic where biofilms present. The use natural compounds, formed situ nontoxic abundant metal mimics, represents a novel efficient “green” strategy emulate utilize defense system preventing bacterial colonization growth.

Язык: Английский

---

Recent Advancements in Enhancing Antimicrobial Activity of Plant-Derived Polyphenols by Biochemical Means

Horticulturae, Год журнала: 2022, Номер 8(5), С. 401 - 401

Опубликована: Май 3, 2022

Plants are a reservoir of phytochemicals, which known to possess several beneficial health properties. Along with all the secondary metabolites, polyphenols have emerged as potential replacements for synthetic additives due their lower toxicity and fewer side effects. However, controlling microbial growth using these preservatives requires very high doses plant-derived compounds, limits use only specific conditions. Their at concentrations leads unavoidable changes in organoleptic properties foods. Therefore, biochemical modification natural can be promising alternative enhance antimicrobial efficacy compounds/polyphenols. Amongst modifications, low concentration ascorbic acid (AA)–Cu (II), degradation products (DPAA), Maillard reaction (MRPs), laccase–mediator (Lac–Med) horse radish peroxidase (HRP)–H2O2 systems standout. This review reveals importance plant polyphenols, role agents, mechanism methods ways may used enhancing potency polyphenols. Ultimately, this study act base development potent agents that find food applications.

Язык: Английский

---

Structural insights, biocatalytic characteristics, and application prospects of lignin-modifying enzymes for sustainable biotechnology

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 242, С. 124968 - 124968

Опубликована: Май 20, 2023

Lignin modifying enzymes (LMEs) have gained widespread recognition in depolymerization of lignin polymers by oxidative cleavage. LMEs are a robust class biocatalysts that include peroxidase (LiP), manganese (MnP), versatile (VP), laccase (LAC), and dye-decolorizing (DyP). Members the family act on phenolic, non-phenolic substrates been widely researched for valorization lignin, cleavage xenobiotics phenolics. implementation biotechnological industrial sectors has sparked significant attention, although its potential future applications remain underexploited. To understand mechanism sustainable pollution mitigation, several studies undertaken to assess feasibility correlating diverse pollutants binding intermolecular interactions at molecular level. However, further investigation is required fully comprehend underlying mechanism. In this review we presented key structural functional features LMEs, including computational aspects, as well advanced biotechnology research. Furthermore, concluding remarks look ahead, use coupled with framework, built upon artificial intelligence (AI) machine learning (ML), emphasized recent milestone environmental

Язык: Английский

---

A Key Role for Apoplastic H₂O₂ in Norway Spruce Phenolic Metabolism

PLANT PHYSIOLOGY, Год журнала: 2017, Номер 174(3), С. 1449 - 1475

Опубликована: Май 18, 2017

Apoplastic events such as monolignol oxidation and lignin polymerization are difficult to study in intact trees. To investigate the role of apoplastic hydrogen peroxide (H2O2) gymnosperm phenolic metabolism, an extracellular lignin-forming cell culture Norway spruce (Picea abies) was used a research model. Scavenging H2O2 by potassium iodide repressed formation, line with peroxidases activating monolignols for polymerization. Time-course analyses coupled candidate substrate-product pair network propagation revealed differential accumulation low-molecular-weight phenolics, including (glycosylated) oligolignols, flavonoids, proanthocyanidins, H2O2-scavenging cultures supported that oxidatively not only wall but also cytoplasm, where they other proanthocyanidins. Dilignol glycoconjugates reduced structures were found medium, suggesting cells able transport glycosylated dilignols apoplast. Transcriptomic scavenging resulted remodulation transcriptome, carbon flux into shikimate pathway propagating down biosynthesis. Aggregated coexpression analysis identified enzymes transcription factors production addition potential receptors. The results presented indicate redox state apoplast has profound influence on cellular metabolism.

Язык: Английский

---