Structure and function of the soil microbiome underlying N2O emissions from global wetlands

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Март 17, 2022

Abstract Wetland soils are the greatest source of nitrous oxide (N 2 O), a critical greenhouse gas and ozone depleter released by microbes. Yet, microbial players processes underlying N O emissions from wetland poorly understood. Using in situ measurements determining structure potential functional communities 645 soil samples globally, we examined role archaea, bacteria, fungi nitrogen (N) cycling emissions. We show that higher drained warm soils, correlated with diversity further provide evidence despite their much lower abundance compared to nitrifying archaeal is key factor explaining globally. Our data suggest ongoing global warming intensifying environmental change may boost nitrifiers, collectively transforming greater O.

Язык: Английский

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Microplastics reduce nitrogen uptake in peanut plants by damaging root cells and impairing soil nitrogen cycling

Journal of Hazardous Materials, Год журнала: 2022, Номер 443, С. 130384 - 130384

Опубликована: Ноя. 11, 2022

Язык: Английский

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Genomic structure predicts metabolite dynamics in microbial communities

Cell, Год журнала: 2022, Номер 185(3), С. 530 - 546.e25

Опубликована: Янв. 31, 2022

Язык: Английский

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Denitrifying communities enriched with mixed nitrogen oxides preferentially reduce N2O under conditions of electron competition in wastewater

Chemical Engineering Journal, Год журнала: 2024, Номер 498, С. 155292 - 155292

Опубликована: Авг. 30, 2024

The reduction rate of nitrous oxide (N2O) is affected by the electron competition among four denitrifying steps, limiting mitigation N2O emissions during wastewater treatment. We foresee essential to understand how combinations acceptors (EAs) affect microbial composition and mechanisms communities. enriched three communities from activated sludge biomass with equivalent loads different EAs: NO3– (R1), (R2), + (R3). resulting enrichments were compared in terms (1) nitrogen oxides absence/presence other EAs (NO3–, NO2–, N2O), (2) their denitrification gene (3) community composition. Batch results showed presence NO2– suppressed rates all reactors. effect was lower mixed-substrate feed than single-substrate under infinite retention time chemostat operation modes. N2O-reducers type nosZ II when serves as sole EA, whereas I more prone enrichment EA. EA rather mode differentiated genus Flavobacterium seems play a significant role alleviating suppression caused competition. Limited conditions supply are norm independently high C/N levels, co-enriched alleviates for electrons reductase enzyme or individually.

Язык: Английский

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Ecological interactions and the underlying mechanism of anammox and denitrification across the anammox enrichment with eutrophic lake sediments

Microbiome, Год журнала: 2023, Номер 11(1)

Опубликована: Апрель 20, 2023

Abstract Background Increasing attention has recently been devoted to the anaerobic ammonium oxidation (anammox) in eutrophic lakes due its potential key functions nitrogen (N) removal for eutrophication control. However, successful enrichment of anammox bacteria from lake sediments is still challenging, partly ecological interactions between and denitrifying across such with remain unclear. Results This study thus designed fill knowledge gaps using bioreactors enrich more than 365 days. We continuously monitored influent effluent water, measured denitrification efficiencies, quantified bacteria, as well related N cycling genes. found that maximum efficiencies NH 4 + NO 2 − reached up 85.92% 95.34%, respectively. Accordingly, diversity decreased significantly enrichment, relative dominant (e.g., Candidatus Jettenia) Thauera, Afipia ) shifted considerably. The cooperation tended increase microbial community stability, indicating a coupling bacteria. Moreover, nirS -type denitrifiers showed stronger nirK during enrichment. Functional potentials depicted by metagenome sequencing confirmed denitrification. Metagenome-assembled genomes-based model indicated most could provide various materials amino acid, cofactors, vitamin Cross-feeding highlights importance increasing lakes. Conclusions greatly expands our understanding mechanisms among sediments, which sheds new insights into controlling eutrophication.

Язык: Английский

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Intermediate soil acidification induces highest nitrous oxide emissions

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 27, 2024

Abstract Global potent greenhouse gas nitrous oxide (N 2 O) emissions from soil are accelerating, with increases in the proportion of reactive nitrogen emitted as N O, i.e., O emission factor (EF). Yet, primary controls and underlying mechanisms EFs remain unresolved. Based on two independent but complementary global syntheses, three field studies determining effects acidity denitrifying microorganisms, we show that pH predominantly by affecting denitrifier community composition. Analysis 5438 paired data points fluxes revealed a hump-shaped relationship between EFs, highest occurring moderately acidic soils favored O-producing over O-consuming induced high emissions. Our results illustrate has unimodal denitrifiers net depends both O/(N + ) ratio overall denitrification rate. These findings can inform strategies to predict mitigate under future input scenarios.

Язык: Английский

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Effect of antibiotic perturbation on nitrous oxide emissions: An in-depth analysis

Critical Reviews in Environmental Science and Technology, Год журнала: 2024, Номер 54(22), С. 1612 - 1632

Опубликована: Апрель 16, 2024

Agricultural practices such as extensive manure application (containing residual antibiotics) fertilizer can also contribute to antibiotic pollution in soil, besides nitrous oxide (N2O) emissions. This dual impact designates certain agricultural soils hotspots for heightened N2O emissions and pollution. Nevertheless, our understanding of the repercussions residues on underlying mechanisms remain elusive. comprehensive literature review investigated recent research findings regarding contamination its influence microbial production consumption. We scrutinized published data field experimental monitoring results analyze prevalence temporal variation Chinese soils. By analyzing 482 log response ratio (lnR) datasets obtained from 41 independent articles, we established that quinolones (QNs) significantly (p < 0.05) boost Conversely, cycloheximide aminoglycosides (ARs) demonstrated notable inhibitory effects. Antibiotic at concentrations within 1 ppm promoted soil emissions, whereas exceeding 10 (95% CI > 0) inhibited Subsequently, changes oxidation-related microorganisms (AOM), nitrite-oxidizing bacteria (NOB), comammox bacteria, denitrifying well dissimilatory nitrate reduction ammonium (DNRA) anammox processes under stress. Antibiotics unequally affect Our offer valuable insights into complex relationship between antibiotics contributing a better environmental consequences systems.

Язык: Английский

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Year-Round Shotgun Metagenomes Reveal Stable Microbial Communities in Agricultural Soils and Novel Ammonia Oxidizers Responding to Fertilization

Applied and Environmental Microbiology, Год журнала: 2017, Номер 84(2)

Опубликована: Ноя. 3, 2017

ABSTRACT The dynamics of individual microbial populations and their gene functions in agricultural soils, especially after major activities such as nitrogen (N) fertilization, remain elusive but are important for a better understanding nutrient cycling. Here, we analyzed 20 short-read metagenomes collected at four time points during 1 year from two depths (0 to 5 30 cm) Midwestern sites representing contrasting soil textures (sandy versus silty loam) with similar cropping histories. Although the community taxonomic functional compositions differed between locations depths, they were more stable within depth/site throughout than communities natural aquatic ecosystems. For example, among 69 population genomes assembled metagenomes, 75% showed less 2-fold change abundance any sampling points. Interestingly, six deep-branching Thaumarchaeota three complete ammonia oxidizer (comammox) Nitrospira increased up 5-fold upon addition N fertilizer. These results indicated that indigenous archaeal oxidizers may respond faster (are copiotrophic) fertilization previously thought. None 29 recovered putative denitrifier encoded denitrification pathway, suggesting is carried out by collection different populations. Altogether, our study identified novel genes responding seasonal human-induced perturbations soils should facilitate future monitoring efforts N-related studies. IMPORTANCE Even though impact management on structure has been recognized, an what each carries limited. Yet, this information cycling, potentially implications preserving sustainability. show reconstructed metagenome-assembled (MAGs) relatively content round, selected comammox nitrifiers oligotrophic marine counterparts studied.

Язык: Английский

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Nitrous Oxide Reduction by an Obligate Aerobic Bacterium, Gemmatimonas aurantiaca Strain T-27

Applied and Environmental Microbiology, Год журнала: 2017, Номер 83(12)

Опубликована: Апрель 8, 2017

N2O-reducing organisms with nitrous oxide reductases (NosZ) are known as the only biological sink of N2O in environment. Among most abundant nosZ genes found environment affiliated understudied Gemmatimonadetes phylum. In this study, a unique regulatory mechanism reduction Gemmatimonas aurantiaca strain T-27, an isolate phylum, was examined. Strain T-27 incubated and/or O2 electron acceptor. Significant observed when initially present. When batch cultures were amended and N2O, commenced after depleted. long-term incubation addition upon depletion, rate decreased over time came to eventual stop. Spiking culture resulted resuscitation activity, supporting hypothesis that by required transient presence The highest level transcription (8.97 transcripts/recA transcript) immediately ∼25-fold within 85 h, phenotype. difference between responses without starvation suggested helped sustain viability during temporary anoxia, although not coupled growth. findings study suggest obligate aerobic microorganisms may utilize surrogate for survive periodic anoxia.IMPORTANCE Emission potent greenhouse gas ozone depletion agent, from soil is largely determined microbial sources sinks. at environmentally relevant concentrations (up ∼1,000 parts per million volume [ppmv]). Although large fraction recovered genomes phylum Gemmatimonadetes, has yet been confirmed any these organisms. This demonstrates reduced bacterium, suggests novel regulation organism, which also be applicable other possessing genes. We expect will significantly advance understanding dynamics environments frequent transitions oxic anoxic conditions.

Язык: Английский

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Relative abundance of denitrifying and DNRA bacteria and their activity determine nitrogen retention or loss in agricultural soil

Soil Biology and Biochemistry, Год журнала: 2018, Номер 123, С. 97 - 104

Опубликована: Май 29, 2018

Язык: Английский

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