Genetic Status of the Swedish Central collection of heirloom apple cultivars

Scientia Horticulturae, Год журнала: 2020, Номер 272, С. 109599 - 109599

Опубликована: Июль 17, 2020

Cultivated apple is one of the most widely grown fruit crops worldwide.With introduction modern cultivars, from foreign and national breeding programs, use local cultivars decreased during 20th century.In order to minimize genetic erosion avoid loss special genotypes, a number clonal archives were established across Sweden, with goal retaining old cultivars.About 220 appointed for preservation, obtained status mandate cultivars.Initially, they identified based on pomological traits, but prior establishment Swedish Central Collection genotyped simple sequence repeat (SSR) markers.SSR markers helped evaluate preserved material, as well find best possible true-to-type source propagation, thus guiding Collection.Recently, 215 accessions this collection using 20 K Infinium® single nucleotide polymorphism (SNP) array, in gain insight into its structure.The initial SSR analysis confirmed identity multiple samples same cultivar name different locations several mislabeled samples.In subsequent SNP we 30 relationships parent-offspring relationships, including 18 trios.We also five inconsistent ploidy levels between data, some cases indicating problematic either or archives.These need further investigation ensure their true-to-typeness.Furthermore, has continued grow since onset work now contains additional which should be included future studies.The results indicate that holds high potential value programs.

Язык: Английский

---

The cherry 6+9K SNP array: a cost-effective improvement to the cherry 6K SNP array for genetic studies

Scientific Reports, Год журнала: 2020, Номер 10(1)

Опубликована: Май 6, 2020

Abstract Cherry breeding and genetic studies can benefit from genome-wide marker assays. Currently, a 6K SNP array enables genome scans in cherry; however, only third of these SNPs are informative, with low coverage many genomic regions. Adding previously detected to this could provide cost-efficient upgrade increased across the 670 cM/352.9 Mb cherry whole sequence. For sweet cherry, new were chosen following focal point strategy, grouping six eight within 10-kb windows an average 0.6 cM (627 kb) between points. Additional represent important Sweet fruticosa subgenome sour organellar genomes targeted 6942, 2020, 38 SNPs, respectively. The +9K add-on provided 2128, 1091, 70 reliable, polymorphic for avium subgenomes 1241 reliable formed 237 informative points, another 2504 in-between. resolution original will help increase understanding control key traits relationships among individuals cherry.

Язык: Английский

---

Detection of QTL for apple fruit acidity and sweetness using sensorial evaluation in multiple pedigreed full-sib families

Tree Genetics & Genomes, Год журнала: 2020, Номер 16(5)

Опубликована: Сен. 8, 2020

Язык: Английский

---

Detection of Breeding-Relevant Fruit Cracking and Fruit Firmness Quantitative Trait Loci in Sweet Cherry via Pedigree-Based and Genome-Wide Association Approaches

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Март 2, 2022

Breeding for decreased fruit cracking incidence and increased firmness in sweet cherry creates an attractive alternative to variable results from cultural management practices. DNA-informed breeding increases its efficiency, yet upstream research is needed identify the genomic regions associated with trait variation of a breeding-relevant magnitude, as well parental sources favorable alleles. The objectives this were quantitative loci (QTLs) firmness, estimate effects single nucleotide polymorphism (SNP) haplotypes at detected QTLs, ancestral source(s) functional haplotypes. Fruit evaluated multiple years on 259 unselected seedlings representing 22 important parents. Phenotypic data, conjunction genome-wide genotypic data RosBREED 6K SNP array, used QTL analysis performed via Pedigree-Based Analysis using FlexQTL™ software, supplemented by Genome-Wide Association Study BLINK software. Haplotype was conducted QTLs their phenotypic effects, tracked through pedigree. Four (two per trait) consistent across and/or both methods validated previously reported QTLs. qCrack-LG1.1m (the label given chromosome 1) explained 2-15.1% variance, while qCrack-LG5.1m, qFirm-LG1.2m, qFirm-LG3.2m 7.6-13.8, 8.8-21.8, 1.7-10.1% respectively. At each QTL, least two had significant considered putative Putative low-cracking unnamed parent 'Emperor Francis' 'Schmidt' parents 'Napoleon' 'Hedelfingen,' among others, high-firmness 'Schmidt,' grandparent 'Black Republican,' 'Rube,' unknown 'Napoleon.' These four stable can now be targeted DNA test development, goal translating information discovered here into usable tools aid decisions.

Язык: Английский

---

Tracing the color: quantitative trait loci analysis reveals new insights into red-flesh pigmentation in apple (Malus domestica)

Horticulture Research, Год журнала: 2024, Номер 11(8)

Опубликована: Июнь 27, 2024

Abstract Red-flesh color development in apple fruit is known to depend upon a particular allele of the MdMYB10 gene. While anthocyanin metabolic pathway well characterized, current genetic models do not explain observed variations red-flesh pigmentation intensity. Previous studies focused on total content as phenotypic trait characterize overall flesh color. this approach led global understanding mechanisms involved expression, it essential adopt more quantitative approach, by analyzing other phenolic compound classes, order better understand molecular subtle variation and distribution. In study, we performed pedigree-based loci (QTL) mapping, using FlexQTL™ software, decipher determinism five F1 inter-connected families segregating for trait. A 452 genotypes were evaluated profiles during 3 years (2021–2023). We identified 24 QTLs intensity profiles. Six detected LG1, LG2, LG8, LG9, LG11, LG16. Several genes QTL confidence intervals related metabolism. Further analyses allowed us propose model which competition between anthocyanins flavan-3-ols (monomer oligomer) end-products decisive development. model, alleles favorable high can be inherited from both white-flesh parents.

Язык: Английский

---