Chiang Mai Journal of Science,
Год журнала:
2022,
Номер
49(3), С. 524 - 537
Опубликована: Май 26, 2022
The
rapid
expansion
of
the
agricultural
fi
eld
has
had
negative
impacts
on
soil
ecological
functions.
Soil
fungi
play
an
important
role
in
nutrient
cycling
and
maintenance
functions
terrestrial
ecosystems.
However,
our
understanding
fungal
communities
interaction
among
microbial
taxa
plant
protected
areas
is
still
limited.
This
study
applied
high-throughput
sequencing
investigation
their
diversity
within
Songkhla
zoo
Southern
Thailand.
sampling
sites
fell
into
three
zones:
zone
I
(dry
evergreen
forest,
mixed
deciduous
forest
eld);
II
(evergreen
forest);
III
rubber
tree
crop).
samples
were
collected
from
each
subjected
to
physicochemical
tests.
Total
DNA
was
extracted
samples,
we
analyzed
nuclear
ribosomal
internal
transcribed
spacer
1
(ITS1)
region
rDNA
gene
by
Illumina
Miseq
assess
communities.
parameters
revealed
that
total
calcium
(Ca),
manganese
(Mg)
potassium
(K)
signifi
cantly
higher
than
other
zones.
A
637,
702
745
OTUs
observed
I,
respectively,
with
143
common
across
all
sites.
different
between
Zone
highest
levels
community
richness
diversity,
followed
I.
dominant
genera
zones
Beauveria,
Staphylotrichum
Trichoderma,
respectively.
Furthermore,
high
concentration
K,
Ca
Mg
affected
zoo.
Our
ndings
provide
insights
importance
fungi,
this
identifi
ed
key
differences
Egyptian Journal of Biological Pest Control,
Год журнала:
2024,
Номер
34(1)
Опубликована: Янв. 30, 2024
Abstract
Background
The
genus
Beauveria
(Bals.)
Vuill.
includes
many
species,
some
of
which
are
limited
to
specific
regions
while
others
distributed
worldwide.
diversity
species
is
poorly
investigated
in
Syria
and
most
studies
lack
proper
diagnosis
species.
Entomopathogenic
isolates
this
were
obtained
using
the
Galleria
Bait
Method.
This
study
aimed
identify
these
based
on
morphological
characterizations
combined
with
molecular
data,
nuclear
ribosomal
internal
transcribed
spacer
(ITS)
elongation
factor
1-alpha
(EF1-α)
sequences.
has
also
been
evaluated
a
phylogenetic
analysis
available
ITS
sequences
Syrian
GenBank.
Results
Two
entomopathogenic
isolates,
B195
B243,
detected
soil
agro-ecosystems
coastal
region.
Morphological
information
revealed
that
two
belong
bassiana
(Hypocreales:
Cordycipitaceae)
514
bp
284
for
each
isolate
EF1-α,
respectively.
Pathogenicity
test
showed
100%
mortality
mellonella
L.
larvae
2–3
days
post-fungal
exposure
both
isolates.
tree
all
clustered
within
B.
,
considerable
intraspecific
diversity,
except
previously
identified
as
closely
related
Beuveria
pseudobassiana
S.A.
Rehner
Humber.
Conclusions
presents
morpho-molecular
characterization
soil-sourced
highly
pathogenic
G.
clarifies
their
placement.
Depending
our
findings,
further
exploration
still
needed
better
understanding
distribution
entomopathogen
Syria.
Entomological Research,
Год журнала:
2024,
Номер
54(4)
Опубликована: Апрель 1, 2024
Abstract
Melon
thrips,
Thrips
palmi
(Thysanoptera:
Thripidae),
is
a
serious
insect
pest
in
crop
productions,
and
synthetic
chemicals
are
frequently
used
to
control
them.
However,
this
practice
causes
residual
issue
nature
makes
melon
thrips
acquire
strong
resistance.
To
overcome
these
problems,
group
of
entomopathogenic
fungi
could
be
as
an
alternative
for
controlling
thrips.
In
work,
total
100
fungal
isolates
from
soil
four
different
regions
Korea
were
isolated
subjected
virulence
assays
against
Twenty‐five
highly
virulent
isolates,
which
showed
100%
mortality
6
days
after
treatment,
determined.
Liquid‐cultured
filtrates
the
selected
further
investigate
their
insecticidal
activity
25
finally
seven
including
Metarhizium
spp.
Fusarium
high
dose‐dependent
manner.
Under
thermal
stress
at
121°C,
culture
still
kept
activities.
When
liquid‐cultured
spores
exposed
45°C,
resistant
stress,
but
those
susceptible
stress.
The
did
not
show
any
phytotoxicity
tomato
plants,
followed
by
no
spores,
although
mycotoxins
need
clearly
characterized.
This
work
suggests
that
competitive
production
metabolites
compared
,
particularly
attractive
sporal
resistance
significantly
stronger
than
.
Chiang Mai Journal of Science,
Год журнала:
2022,
Номер
49(3), С. 524 - 537
Опубликована: Май 26, 2022
The
rapid
expansion
of
the
agricultural
fi
eld
has
had
negative
impacts
on
soil
ecological
functions.
Soil
fungi
play
an
important
role
in
nutrient
cycling
and
maintenance
functions
terrestrial
ecosystems.
However,
our
understanding
fungal
communities
interaction
among
microbial
taxa
plant
protected
areas
is
still
limited.
This
study
applied
high-throughput
sequencing
investigation
their
diversity
within
Songkhla
zoo
Southern
Thailand.
sampling
sites
fell
into
three
zones:
zone
I
(dry
evergreen
forest,
mixed
deciduous
forest
eld);
II
(evergreen
forest);
III
rubber
tree
crop).
samples
were
collected
from
each
subjected
to
physicochemical
tests.
Total
DNA
was
extracted
samples,
we
analyzed
nuclear
ribosomal
internal
transcribed
spacer
1
(ITS1)
region
rDNA
gene
by
Illumina
Miseq
assess
communities.
parameters
revealed
that
total
calcium
(Ca),
manganese
(Mg)
potassium
(K)
signifi
cantly
higher
than
other
zones.
A
637,
702
745
OTUs
observed
I,
respectively,
with
143
common
across
all
sites.
different
between
Zone
highest
levels
community
richness
diversity,
followed
I.
dominant
genera
zones
Beauveria,
Staphylotrichum
Trichoderma,
respectively.
Furthermore,
high
concentration
K,
Ca
Mg
affected
zoo.
Our
ndings
provide
insights
importance
fungi,
this
identifi
ed
key
differences
