Diversity and structure of the root-associated bacterial microbiomes of four mangrove tree species, revealed by high-throughput sequencing

PeerJ, Год журнала: 2023, Номер 11, С. e16156 - e16156

Опубликована: Окт. 4, 2023

Root-associated microbes of the mangrove trees play important roles in protecting and maintaining ecosystems. At present, most our understanding root-related microbial diversity is obtained from specific species selected geographic regions. Relatively little known about composition bacterial microbiota existing disparate microenvironments, particularly relationship among different tropical environments.We collected root, rhizosphere soil, non-rhizosphere soil four (Acanthus ilicifolius, Bruguiera gymnorrhiza, Clerodendrum inerme, Lumnitzera racemosa) detected 16S rRNA gene by a conventional PCR. We performed high throughput sequencing using Illumina Novaseq 6000 platform (2 × 250 paired ends) to investigate communities related with species.We analyzed diverse ecological niches species. Our data confirmed distinct distribution patterns three rhizocompartments Microbiome varied compartments host The between endosphere other two were distinctly independent large degree overlap critical community members same rhizocompartment across was found at phylum level. Furthermore, this first report Acidothermus environments. In conclusion, complicated host-microbe associations could lay foundation for exploitation resource production secondary metabolites.

Язык: Английский

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Microbial communities in swamps of four mangrove reserves driven by interactions between physicochemical properties and microbe in the North Beibu Gulf, China

Environmental Science and Pollution Research, Год журнала: 2022, Номер 29(25), С. 37582 - 37597

Опубликована: Янв. 23, 2022

Язык: Английский

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Microbial Detoxification of Sediments Underpins Persistence of <em>Zostera marina</em> Meadows

Опубликована: Апрель 15, 2024

Eelgrass meadows have attracted a lot of attention not only for their ability to maintain marine ecosystems as feeding grounds organisms, but also potential store atmos-pheric and dissolved CO2 blue carbon. This study comprehensively evaluated the bacterial chemical composition data obtained from eelgrass sediments different scales along coast Japan investigate sediment effect on acclimatization eelgrass. Regardless habitat, approximately 1% Anaerolineales, Babeliales, Cytophagales, Phycisphaerales were present in bottom sediment. Sulfate-reducing bacteria (SRB) at 3.69 % compared 1.70 bare Sulfur-oxidizing (SOB) 2.81% 1.10% sediment, respectively. Bacterial analysis linear discriminant revealed that sulfur-oxidizing detoxified H2S larger-scale had higher diversity sul-fur-oxidizing bacteria. Our result indicated one following systems detoxifies microbial oxidation by bacteria, O2 perme-ated physical diffusion benthos. issues maintaining sustain-able conservation bed.

Язык: Английский

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Microbial Detoxification of Sediments Underpins Persistence of Zostera marina Meadows

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5442 - 5442

Опубликована: Май 16, 2024

Eelgrass meadows have attracted much attention not only for their ability to maintain marine ecosystems as feeding grounds organisms but also potential store atmospheric and dissolved CO2 blue carbon. This study comprehensively evaluated the bacterial chemical data obtained from eelgrass sediments of different scales along Japanese coast investigate effect on acclimatization eelgrass. Regardless habitat, approximately 1% Anaerolineales, Babeliales, Cytophagales, Phycisphaerales was present in bottom sediment. Sulfate-reducing bacteria (SRB) were at 3.69% sediment compared 1.70% bare Sulfur-oxidizing (SOB) 2.81% 1.10% sediment, respectively. Bacterial composition analysis linear discriminant revealed that SOB detoxified H2S larger-scale had a higher diversity SOB. Our result indicated there regional differences system detoxifies meadows, either microbial oxidation mediated by or O2 permeation via physical diffusion benthos. However, since flora phylogenetic analyses cannot show bias and/or causality due PCR, future kinetic studies metabolism are expected.

Язык: Английский

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Beta diversity patterns and driving mechanisms of stream bacteria and fungi on Mt. Kilimanjaro

Ecological Informatics, Год журнала: 2024, Номер 82, С. 102747 - 102747

Опубликована: Июль 29, 2024

Aquatic microbes, which are highly diverse in freshwater biofilms, play a vital role shaping the structure and functioning of ecosystems. Nevertheless, influence environmental factors on aquatic microbes different biogeographic ecosystems, especially tropical mountains, is relatively unexplored. Here, we examined relative climate physicochemical beta diversity stream bacteria fungi using 16S rRNA amplicon sequencing at 40 sites Mt. Kilimanjaro. Towards larger elevational distance, total turnover increased significantly, while nestedness decreased. The showed greater spatial variation than that bacteria, local contribution to (LCBD) U-shaped pattern for both taxa. Bacteria had strong positive relationships regarding diversity, LCBD their components, except nestedness, indicating similar preferences resource overlap. Among variables, mean annual temperature, water depth elevation predicted microbial depth, temperature precipitation primarily affected LCBD. Overall, climate-related variables were dominant effects more pronounced bacteria. contrasting patterns bacterial fungal ecological interactions ecosystems provide rare dataset assessing functions understudied predicting impact global change microorganisms.

Язык: Английский

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