Reverse engineering of a pathogenic antibody reveals the molecular mechanism of vaccine-induced immune thrombotic thrombocytopenia DOI Open Access
Daniil G. Ivanov, Nikola Ivetic, Yi Du

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 1, 2023

Abstract The massive COVID-19 vaccine roll-out campaign illuminated a range of rare side effects, the most dangerous which – vaccine-induced immune thrombotic thrombocytopenia (VITT) is caused by adenoviral (Ad)-vectored vaccines. VITT occurrence had been linked to production pathogenic antibodies that recognize an endogenous chemokine, platelet factor 4 (PF4). Mass spectrometry (MS)-based evaluation ensemble anti-PF4 obtained from patient’s blood indicates its major component monoclonal antibody. Structural characterization this antibody reveals several unusual characteristics, such as presence N -glycan in Fab segment and high density acidic amino acid residues CDR regions. A recombinant version (RVT1) was generated transient expression mammalian cells based on newly determined sequence. It captures key properties antibodies, their ability activate platelets PF4-dependent fashion. Homology modeling well-defined polyanionic paratope, docking studies indicate polycationic PF4 readily accommodates two segments, cross-linking yield polymerized complexes. Their existence verified with native MS detecting assemblies large 3 (PF4) 2 , pointing out at FcγRIIa-mediated activation molecular mechanism underlying clinical manifestations. In addition affinity, RVT1 binds other targets, indicating polyreactive nature This surprising polyspecificity not only sheds light etiology, but also opens up opportunities manage pathology. Significance Statement Vaccine-induced effect adenoviral-vectored vaccines emergence autoantibodies recognizing We have engineered sequencing patient-derived causes triggers thrombosis. used characterize architecture complexes combination biophysical computational approaches, revealing VITT. results work demonstrate critical role electrostatics recognition latter. Availability will be invaluable for future aiming understanding general mechanistic features autoimmune pathologies.

Язык: Английский

Blame Attribution and Compliance with COVID-19 Measures in Australia: The Theory of Planned Behaviour DOI Creative Commons
KyuJin Shim, Dashi Zhang

COVID, Год журнала: 2025, Номер 5(2), С. 14 - 14

Опубликована: Янв. 27, 2025

This study scrutinizes the influence of “blame attribution” and Theory Planned Behaviour (TPB) on compliance with COVID-19 public health measures in Australia. elucidates that blaming individuals rather than governments surprisingly augments support for governmental regulations, highlighting complexities blame attribution shaping adherence to policies. It underscores nuanced roles TPB elements like subjective norms behavioural control, revealing feelings empowerment, social responsibility, recognizing personal pandemic control enhance inclination directives. The outcomes emphasize criticality understanding dynamics devising efficacious communication management strategies, promoting societal essential regulations actions during crises, fostering a more resilient infrastructure dealing pandemics.

Язык: Английский

Процитировано

0
Newness, unfamiliarity, and cultural beliefs; social and behavioural barriers to COVID-19 vaccination among the Dumagat Remontado, an Indigenous population in the Philippines DOI
Carmen Silvestre, Bianca Joyce T Sornillo, Vivienne Endoma

и другие.

Health & Place, Год журнала: 2025, Номер 93, С. 103444 - 103444

Опубликована: Апрель 6, 2025

Язык: Английский

Процитировано

0
The barriers and facilitators of herpes zoster vaccination intentions of urban residents in China: a qualitative study DOI Creative Commons
Beibei Yuan, C. Long, Ming Wang

и другие.

Global Health Research and Policy, Год журнала: 2025, Номер 10(1)

Опубликована: Апрель 18, 2025

Abstract Background In an aging society, herpes zoster (HZ) increases the health burden on infected patients. While quantitative studies point to a lack of willingness accept HZ vaccine in China, there is limited number with in-depth qualitative analysis vaccination intention. This study undertakes qualitive method identify barriers and facilitators behind urban residents’ intention three China cities, contributes towards some targeted promotion suggestions other LMICs similar low coverage vaccination. Methods We conducted 12 focus group discussions cities China. each discussion we recruited 3 6 participants aged 20 older catch views from residents wider age range. Participants were by purposive sampling techniques. Guided belief model, thematic was used participants’ attitudes Results The attitude 59 participating showed low-level acceptability only 27.1% (16/59) displaying uptake. included or incorrect conception prevalence, risk factors, susceptibility, symptoms, prevention treatment methods, high cost vaccine. Perceived vulnerability HZ, fear pain individuals' financial capacity strongest addition, it found that advocacy professionals government subsidies vaccination, could attenuate above Conclusions Our revealed series recommend education workers officials address knowledge misconceptions, (or insurance providers) pay subsidize costs increase rate.

Язык: Английский

Процитировано

0
COVID-19 Information Overload Mediated the Effects of Cross-Channel Information Differences on Health Information Elaboration DOI
Qian Huang, Bingjing Mao, Xiaofeng Jia

и другие.

Journal of Health Communication, Год журнала: 2023, Номер 28(7), С. 401 - 411

Опубликована: Май 26, 2023

The COVID-19 pandemic has brought on an unprecedented amount of information about the virus and vaccination, varying significantly across channels. While extant research shows that excessive leads to overload less elaboration, few studies have examined factors associated with elaboration. Considering trend we likely receive same topics from different communication channels daily, this study sought understand how cross-channel differences in were subsequent survey assessed 471 participants' consumption (interpersonal vs. social media), concern quality, overload, health literacy, demographic characteristics February 2021. Our findings confirmed greater was negatively more Using a moderated mediation model, found people who received media, compared those equal amounts both media interpersonal communications, reported Additionally, experienced held quality tended elaborate information. All analyses controlled for literacy. Theoretical practical implications discussed.

Язык: Английский

Процитировано

10
Reverse Engineering of a Pathogenic Antibody Reveals the Molecular Mechanism of Vaccine-Induced Immune Thrombotic Thrombocytopenia DOI
Daniil G. Ivanov, Nikola Ivetic, Yi Du

и другие.

Journal of the American Chemical Society, Год журнала: 2023, Номер 145(46), С. 25203 - 25213

Опубликована: Ноя. 10, 2023

The massive COVID-19 vaccine roll-out campaign illuminated a range of rare side effects, the most dangerous which─vaccine-induced immune thrombotic thrombocytopenia (VITT)─is caused by adenoviral (Ad)-vectored vaccines. VITT occurrence had been linked to production pathogenic antibodies that recognize an endogenous chemokine, platelet factor 4 (PF4). Mass spectrometry (MS)-based evaluation ensemble anti-PF4 obtained from patient's blood indicates major component is monoclonal antibody. Structural characterization this antibody reveals several unusual characteristics, such as presence N-glycan in Fab segment and high density acidic amino acid residues complementarity-determining regions. A recombinant version (RVT1) was generated transient expression mammalian cells based on newly determined sequence. It captures key properties their ability activate platelets PF4 concentration-dependent fashion. Homology modeling well-defined polyanionic paratope, docking studies indicate polycationic readily accommodates two segments, cross-linking yield polymerized complexes. Their existence verified with native MS detecting assemblies large (RVT1)3(PF4)2, pointing out at FcγRIIa-mediated activation molecular mechanism underlying clinical manifestations. In addition affinity, RVT1 binds other targets, indicating polyreactive nature This surprising promiscuity not only sheds light etiology but also opens up opportunities manage pathology.

Язык: Английский

Процитировано

10
Structural Characterization of a Pathogenic Antibody Underlying Vaccine-Induced Immune Thrombotic Thrombocytopenia (VITT) DOI
Son Nguyen, Si-Hung Le, Daniil G. Ivanov

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(16), С. 6209 - 6217

Опубликована: Апрель 12, 2024

Vaccine-induced immune thrombotic thrombocytopenia (VITT) is a rare but dangerous side effect of adenoviral-vectored COVID-19 vaccines. VITT had been linked to production autoantibodies recognizing platelet factor 4 (PF4). Here, we characterize anti-PF4 antibodies obtained from patient's blood. Intact mass measurements indicate that significant fraction these represent limited number clones. MS analysis large antibody fragments (the light chain and the Fc/2 Fd heavy chain) confirms monoclonal nature this component repertoire reveals presence mature complex biantennary

Язык: Английский

Процитировано

2
Factors that influence recruitment to COVID-19 vaccine trials: a qualitative evidence synthesis DOI Creative Commons
Linda Biesty, Sarah Sheehan, Pauline Meskell

и другие.

Trials, Год журнала: 2024, Номер 25(1)

Опубликована: Дек. 19, 2024

The COVID-19 pandemic marked a unique period characterised by an extraordinary global virus spread. collective effort to halt the transmission of led various public health initiatives, including variety vaccine trials. Many these trials used adaptive methods address pandemic's challenges, such as need for rapid recruitment. These allow modifications trial procedures without undermining trial's integrity, making research process more flexible and efficient. However, recruiting participants remains considerable challenge. aim this qualitative evidence synthesis (QES) is explore factors that influence person's decision participate in trial. Lessons learned from could help shape future trials' design conduct, particularly those conducted within pandemic. We systematic search studies mixed with component WHO Research Database, MEDLINE, CINAHL, PsycINFO, Epistemomikos, Online Resource Clinical Trials (ORCCA), Cochrane Study Register. best-fit framework approach Social Ecological Model priori framework. GRADE-CERQual assess our confidence review findings. Five involving 539 were included. One included In three studies, asked hypothetically about their attitudes. Another study people who had either not responded or declined invitation developed six themes outlining trial: (1) personal gains, (2) perceived risk, (3) family community, (4) contributing others, (5) institutional trust mistrust, (6) accessibility This sheds light on how perceive potential personal, family, community advantages participation perceptions may be weighed against concerns safety. findings also point toward specific aspects methodology consider when designing

Язык: Английский

Процитировано

2
Messaging Preferences about the COVID-19 Vaccine among Adults in Eastern North Carolina DOI Creative Commons
Abby Schwartz, Alice R. Richman, Essie Torres

и другие.

Journal of Community Health, Год журнала: 2024, Номер 50(1), С. 71 - 80

Опубликована: Сен. 5, 2024

Язык: Английский

Процитировано

0
COVID-19 Testing Behavior as a Predictor of COVID-19 Vaccination in Southeastern Louisiana: A Longitudinal Cohort Study DOI Creative Commons
Sara Al-Dahir,

Saba Barri,

Klaus Heyer

и другие.

Vaccines, Год журнала: 2024, Номер 12(12), С. 1338 - 1338

Опубликована: Ноя. 27, 2024

Background: Global COVID-19 vaccination effort faces the challenges of vaccine hesitancy and resistance, rooted in misinformation institutional distrust. Addressing these barriers with customized messaging is essential, yet relationship between other health-seeking behaviors, like testing, has been underexplored. Method: This study assessed uptake Southeastern Louisiana across 10 pharmacies clinics areas historically high rates infection. Using a longitudinal cohort design from Fall 2022 through 2023, total 377 participants diverse backgrounds were surveyed while seeking routine care at partner organizations. Baseline data was collected on demographics, knowledge, attitudes, test-seeking behaviors. Information testing self-reported verified, as applicable, patient’s medical records. All analyzed using descriptive statistics, log-binomial to yield risk ratios, an ordinal logistic regression for series completion. Results: Among participants, 207 unvaccinated 170 received vaccine. individuals, 53 half-dose, 97 full dose, 14 booster. Notably, 75% 89% vaccinated underwent testing. Individuals who tested 1.71 times more likely be (95% CI 1.03, 2.84), previous refusal associated lower acceptance (0.77; 95% 0.54, 1.09). In bivariate multivariate analysis, behavior positively uptake. Conclusions: Exploring connection provides valuable insights future public health mitigate hesitancy.

Язык: Английский

Процитировано

0
Existential Approaches to Vaccine Hesitancy: Terror Management Theory DOI
Lindsey A. Harvell-Bowman

Risk, systems and decisions, Год журнала: 2024, Номер unknown, С. 135 - 141

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0