bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 26, 2023
Abstract
The
coronavirus
disease
2019
(COVID-19)
pandemic,
caused
by
severe
acute
respiratory
syndrome
2
(SARS-CoV-2)
may
be
over,
but
its
variants
continue
to
emerge,
and
patients
with
mild
symptoms
having
long
COVID
is
still
under
investigation.
SARS-CoV-2
infection
leading
elevated
cytokine
levels
suppressed
immune
responses
set
off
storm,
fatal
systemic
inflammation,
tissue
damage,
multi-organ
failure.
Thus,
drug
molecules
targeting
the
virus-specific
proteins
or
capable
of
suppressing
host
inflammatory
viral
would
provide
an
effective
antiviral
therapy
against
emerging
concern.
Evolutionarily
conserved
papain-like
protease
(PLpro)
main
(Mpro)
play
indispensable
role
in
virus
life
cycle
evasion.
Direct-acting
antivirals
both
these
proteases
represent
attractive
strategy
that
also
expected
reduce
inflammation.
present
study
has
evaluated
anti-inflammatory
potential
natural
triterpenoids:
azadirachtin,
withanolide_A,
isoginkgetin.
These
inhibit
Mpro
PLpro
proteolytic
activities
half-maximal
inhibitory
concentrations
(IC
50
)
values
ranging
from
1.42
32.7
µM.
Isothermal
titration
calorimetry
(ITC)
analysis
validated
binding
compounds
PLpro.
As
expected,
two
compounds,
withanolide_A
exhibit
potent
anti-SARS-CoV-2
activity
cell-based
assays,
half-
maximum
concentration
(EC
21.73
µM
31.19
µM,
respectively.
azadirachtin
when
assessed
using
HEK293T
cells
were
found
significantly
CXCL10,
TNFα,
IL6,
IL8
cytokines,
which
are
cases
COVID-19.
Interestingly,
rescue
decreased
type-I
interferon
response
(IFN-α1).
results
this
clearly
highlight
triterpenoids
as
target
specific
enzymes
pathways
involved
mediated
Polymers for Advanced Technologies,
Год журнала:
2024,
Номер
35(11)
Опубликована: Ноя. 1, 2024
ABSTRACT
This
research
investigates
the
possible
use
of
polycaprolactone
(PCL)
nanofiber
loaded
with
different
concentrations
clove
oil
(CO)
as
an
antimicrobial
bioactive
agent
for
utilization
in
food
packaging.
CO
nanoemulsions
were
prepared
using
various
resulting
small,
spherically‐shaped
particles.
The
codes
(CO‐1,
CO‐2,
and
CO‐3)
given
to
these
nanoemulsions,
which
had
relative
diameters
69
nm,
71
150
respectively.
After
adding
nanoemulsion
PCL
solutions,
samples
(PCL‐CO
1,
PCL‐CO
2,
3),
showed
contact
angle
values
(131.1°,
125.1°,
117.4°)
than
unmodified
nanofibers
without
(PCL
‐base;
140.5°).
These
obtained
exhibited
smooth
feature.
Additionally,
evaluations
thermal
stability
revealed
that
higher
resistance
when
compared
loading
base).
antibacterial
assessments
conducted
on
their
efficiency
against
typical
spoilage
bacteria,
such
Pseudomonas
aeruginosa
,
Salmonella
enterica
Listeria
monocytogenes
Bacillus
subtilis
improved
concentration
increased.
Surprisingly,
most
inhibitory
impact
was
seen
at
greater
concentrations,
bacterial
counts
being
considerably
reduced
by
6.5
log
10
CFU/mL.
Significantly,
3
composite
encouraging
characteristics
point
its
reducing
growth
microorganisms
packaging
environments.
results
highlight
potential
particular)
multi‐purposes
appropriate
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 26, 2023
Abstract
The
coronavirus
disease
2019
(COVID-19)
pandemic,
caused
by
severe
acute
respiratory
syndrome
2
(SARS-CoV-2)
may
be
over,
but
its
variants
continue
to
emerge,
and
patients
with
mild
symptoms
having
long
COVID
is
still
under
investigation.
SARS-CoV-2
infection
leading
elevated
cytokine
levels
suppressed
immune
responses
set
off
storm,
fatal
systemic
inflammation,
tissue
damage,
multi-organ
failure.
Thus,
drug
molecules
targeting
the
virus-specific
proteins
or
capable
of
suppressing
host
inflammatory
viral
would
provide
an
effective
antiviral
therapy
against
emerging
concern.
Evolutionarily
conserved
papain-like
protease
(PLpro)
main
(Mpro)
play
indispensable
role
in
virus
life
cycle
evasion.
Direct-acting
antivirals
both
these
proteases
represent
attractive
strategy
that
also
expected
reduce
inflammation.
present
study
has
evaluated
anti-inflammatory
potential
natural
triterpenoids:
azadirachtin,
withanolide_A,
isoginkgetin.
These
inhibit
Mpro
PLpro
proteolytic
activities
half-maximal
inhibitory
concentrations
(IC
50
)
values
ranging
from
1.42
32.7
µM.
Isothermal
titration
calorimetry
(ITC)
analysis
validated
binding
compounds
PLpro.
As
expected,
two
compounds,
withanolide_A
exhibit
potent
anti-SARS-CoV-2
activity
cell-based
assays,
half-
maximum
concentration
(EC
21.73
µM
31.19
µM,
respectively.
azadirachtin
when
assessed
using
HEK293T
cells
were
found
significantly
CXCL10,
TNFα,
IL6,
IL8
cytokines,
which
are
cases
COVID-19.
Interestingly,
rescue
decreased
type-I
interferon
response
(IFN-α1).
results
this
clearly
highlight
triterpenoids
as
target
specific
enzymes
pathways
involved
mediated
