Experimental Eye Research, Год журнала: 2023, Номер 238, С. 109748 - 109748
Опубликована: Дек. 9, 2023
Язык: Английский
Progress in Retinal and Eye Research, Год журнала: 2024, Номер 99, С. 101234 - 101234
Опубликована: Янв. 2, 2024
The cornea, as a dynamic and responsive tissue, constantly interacts with mechanical forces in order to maintain its structural integrity, barrier function, transparency refractive power. Cells within the cornea sense respond various that fundamentally regulate their morphology fate development, homeostasis pathophysiology. Corneal cells also dynamically extracellular matrix (ECM) ensuing cell-ECM crosstalk serves signaling reservoir providing biophysical biochemical cues corneal cells. Here we provide an overview of mechanotransduction pathways then delve into recent advances mechanobiology, focusing on interplay between responses epithelial, stromal, endothelial We identify species-specific differences biomechanics facilitate identification optimal animal models study wound healing, disease, novel therapeutic interventions. Finally, key knowledge gaps opportunities mechanobiology are pressing for research community address especially pertinent domains limbal stem cell deficiency, keratoconus Fuchs' dystrophy. By furthering our understanding can contextualize discoveries regarding diseases well innovative treatments them.
Язык: Английский
Процитировано
13
Cells, Год журнала: 2024, Номер 13(14), С. 1218 - 1218
Опубликована: Июль 19, 2024
Descemet’s Stripping Only (DSO) is a surgical technique that utilizes the peripheral corneal endothelial cell (CEnC) migration for wound closure. Ripasudil, Rho-associated protein kinase (ROCK) inhibitor, has shown potential in DSO treatment; however, its mechanism promoting CEnC remains unclear. We observed ripasudil-treated immortalized normal and Fuchs dystrophy (FECD) cells exhibited significantly enhanced healing, particularly effective FECD cells. Ripasudil upregulated mRNA expression of Snail Family Transcriptional Repressor (SNAI1/2) Vimentin (VIM) while decreasing Cadherin (CDH1), indicating endothelial-to-mesenchymal transition (EMT) activation. activated Rac1, driving actin-related complex (ARPC2) to leading edge, facilitating migration. Ex vivo studies on cadaveric membrane (DM) showed increased proliferation CEnCs after ripasudil treatment. An ex model demonstrated from DM stroma with ripasudil. Coating small incision lenticule extraction (SMILE) tissues an FNC coating mix treating conjunction further improved resulted monolayer formation, as detected by ZO-1 junctional marker, thereby reduction EMT. In conclusion, effectively cellular migration, novel model, when stromal microenvironment was modulated. This suggests promising adjuvant treatment, highlighting clinical significance.
Язык: Английский
Процитировано
4
Carbohydrate Polymers, Год журнала: 2024, Номер 346, С. 122596 - 122596
Опубликована: Авг. 8, 2024
Язык: Английский
Процитировано
3
Progress in Retinal and Eye Research, Год журнала: 2023, Номер 96, С. 101192 - 101192
Опубликована: Июнь 30, 2023
Язык: Английский
Процитировано
7
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Май 4, 2024
Abstract Fuchs endothelial corneal dystrophy (FECD) is a complex disease characterized by the progressive decline and morphological changes of cells (CECs) that leads to edema vision loss. The most common mutation in FECD an intronic CTG repeat expansion transcription factor 4 ( TCF4 ) its altered expression. Corneal wound healing occurs primarily through cell enlargement migration, CECs have been shown display increased migration speeds. In this study, we aim determine whether can promote cellular CECs. We generated stable CEC lines derived from patients overexpressed different isoforms investigated epithelial-to-mesenchymal (EMT) expression, analysis found full length TCF4-B isoform overexpression promotes EMT-independent manner. RNA-sequencing identified several pathways including negative regulation microtubules, with TUBB4A (tubulin beta 4A class IVa) as top upregulated gene. expression was ex vivo specimens, there cytoskeleton proteins, tubulin actin, compared normal healthy donor specimens. Additionally, acetylation detyrosination microtubules supporting microtubule stability could migration. Future studies be aimed at investigating if targeting would therapeutic potential for promoting regeneration.
Язык: Английский
Процитировано
2
Cell Proliferation, Год журнала: 2024, Номер 57(7)
Опубликована: Фев. 14, 2024
Abstract A major risk factor for glaucoma, the first leading cause of irreversible blindness worldwide, is decellularisation trabecular meshwork (TM) in conventional outflow pathway. Stem cell‐based therapy, particularly utilisation induced pluripotent stem cells (iPSCs), presents an enticing potential tissue regeneration and intraocular pressure (IOP) maintenance glaucoma. We have previously observed that differentiated iPSCs can stimulate endogenous cell proliferation TM, a pivotal TM aqueous humour restoration. In this study, we investigated response vivo after interacting with iPSC‐derived identified two subpopulations responsible relatively long‐term regeneration: ATP Binding Cassette Subfamily G Member 2 (ABCG2)‐positive Nestin (NES)‐positive cells. further uncovered alterations these responsive are linked to ageing different glaucoma etiologies, suggesting ABCG2 + subpopulation decellularization could serve as Taken together, our findings illustrated proliferative pathway when stimulated defined them precursors, which may be applied develop novel therapeutic approaches
Язык: Английский
Процитировано
1
Case Reports in Ophthalmology, Год журнала: 2024, Номер 15(1), С. 518 - 524
Опубликована: Июнь 26, 2024
Corneal graft detachment is a major postoperative complication of Descemet's stripping automated endothelial keratoplasty (DSAEK). When corneal becomes detached, function generally fails, and repeat transplantation required. Herein, we report rare case in which transparent cornea was maintained after the removal dislocated DSAEK graft.
Язык: Английский
Процитировано
0
Cornea, Год журнала: 2024, Номер unknown
Опубликована: Окт. 29, 2024
Purpose: To examine clinical results up to 10 years after Descemet Membrane Endothelial Keratoplasty (DMEK) and Triple-DMEK. Methods: Prospective study including 201 eyes, of which 54 eyes [38 DMEKs 16 Triple-DMEKs; Fuchs endothelial dystrophy (n = 45), pseudophakic bullous keratopathy 9)] reached the minimum follow-up 5 were followed for years. We evaluated best-corrected visual acuity (BCVA, logarithm angle resolution [logMAR]), cell density (ECD, cells/mm 2 ), minimal central corneal thickness (CCT, μm), retinal (μm), rebubbling, graft survival re-DMEK rate, chamber alterations, incidence glaucoma. Results: Fifty-four had a 5-year 37 an 8- 10-year follow-up. Mean was 94.4 ± 12.1 months. Best-corrected increased from 0.6 0.3 logMAR 0.1 0.2 at 6 months ( P ≤ 0.001) 539 μm long term. decreased 2488 320.9 (donor) 980.1 437 with average ECD loss/year 4% did not correlate BCVA. Central 596.9 82.2 498.6 24.3 12 remained stable 1.000). rebubbling rate 0.5/eye Eight (14.8%) received least 1 rebubbling. Primary failure/rejection 1.5%/0% within first postoperative year, secondary failure 12.4% 7 (preoperative n 3, de novo 5) open-angle glaucoma without changes (14.8%, 0.001). Conclusions: Although decreases continuously in long-term follow-up, excellent can be preserved DMEK The final outcome estimated surgery.
Язык: Английский
Процитировано
0
Experimental Eye Research, Год журнала: 2023, Номер 238, С. 109748 - 109748
Опубликована: Дек. 9, 2023
Язык: Английский
Процитировано
1