Genomic Characterization of Bacillus sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological Potential DOI Creative Commons
Samuel Mwakisha Mwamburi, Sk Injamamul Islam,

Nguyen Dinh‐Hung

и другие.

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2476 - 2476

Опубликована: Дек. 2, 2024

Bacillus sp. THPS1 is a novel strain isolated from high-temperature hot spring in Thailand, exhibiting distinctive genomic features that enable adaptation to an extreme environment. This study aimed characterize the and functional attributes of understand its strategies evaluate potential for biotechnological applications. The draft genome 5.38 Mbp with GC content 35.67%, encoding 5606 genes, including those linked stress response sporulation, which are essential survival conditions. Phylogenetic analysis average nucleotide identity (ANI) values confirmed classification as distinct species within genus. Pangenome involving 19 others closely related thermophilic identified 1888 singleton genes associated heat resistance, specialized metabolism, suggesting nutrient-deficient, environments. Genomic revealed 12 biosynthetic gene clusters (BGCs), polyketides non-ribosomal peptides, highlighting synthesizing secondary metabolites may facilitate adaptation. Additionally, presence three Siphoviridae phage regions 96 mobile genetic elements (MGEs) suggests significant plasticity, whereas existence five CRISPR arrays implies advanced defense mechanism against infections, contributing stability. capacities make it promising candidate applications, particularly production heat-stable enzymes development resilient bioformulations.

Язык: Английский

CRISPR in clinical diagnostics: bridging the gap between research and practice DOI
Zainab Lafi,

Tha’er Ata,

S. Asha

и другие.

Bioanalysis, Год журнала: 2025, Номер unknown, С. 1 - 10

Опубликована: Фев. 4, 2025

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has transformed molecular biology through its precise gene-editing capabilities. Beyond initial applications in genetic modification, emerged as a powerful tool diagnostics and biosensing. This review explores transition from genome editing to innovative detection methods, including nucleic acid identification, single nucleotide polymorphism (SNP) analysis, protein sensing. Advanced technologies such SHERLOCK DETECTR demonstrate CRISPR's potential for point-of-care diagnostics, enabling rapid highly sensitive detection. The integration of chemical modifications, CRISPR-Chip technology, enzymatic systems like Cas12a Cas13a enhances signal amplification efficiency. These advancements promise decentralized, real-time diagnostic solutions with significant implications global healthcare. Furthermore, the fusion artificial intelligence digital health platforms is paving way more accessible, cost-effective, scalable approaches, ultimately revolutionizing precision medicine.

Язык: Английский

Процитировано

1
Reforestation impact on soil bacterial biodiversity antagonists of fungal pathogens in Amazon biome DOI

Tatiana Pereira,

Kaynara F. L. Kavasaki,

Kellen Banhos do Carmo

и другие.

Trees, Год журнала: 2025, Номер 39(1)

Опубликована: Янв. 14, 2025

Язык: Английский

Процитировано

0
Genomic Characterization of Bacillus sp. THPS1: A Hot Spring-Derived Species with Functional Features and Biotechnological Potential DOI Creative Commons
Samuel Mwakisha Mwamburi, Sk Injamamul Islam,

Nguyen Dinh‐Hung

и другие.

Microorganisms, Год журнала: 2024, Номер 12(12), С. 2476 - 2476

Опубликована: Дек. 2, 2024

Bacillus sp. THPS1 is a novel strain isolated from high-temperature hot spring in Thailand, exhibiting distinctive genomic features that enable adaptation to an extreme environment. This study aimed characterize the and functional attributes of understand its strategies evaluate potential for biotechnological applications. The draft genome 5.38 Mbp with GC content 35.67%, encoding 5606 genes, including those linked stress response sporulation, which are essential survival conditions. Phylogenetic analysis average nucleotide identity (ANI) values confirmed classification as distinct species within genus. Pangenome involving 19 others closely related thermophilic identified 1888 singleton genes associated heat resistance, specialized metabolism, suggesting nutrient-deficient, environments. Genomic revealed 12 biosynthetic gene clusters (BGCs), polyketides non-ribosomal peptides, highlighting synthesizing secondary metabolites may facilitate adaptation. Additionally, presence three Siphoviridae phage regions 96 mobile genetic elements (MGEs) suggests significant plasticity, whereas existence five CRISPR arrays implies advanced defense mechanism against infections, contributing stability. capacities make it promising candidate applications, particularly production heat-stable enzymes development resilient bioformulations.

Язык: Английский

Процитировано

0