Characterization of the hepatic flora and metabolome in nonalcoholic fatty liver disease DOI Creative Commons

Hua Jiang,

Hui Wang, Yisong Guo

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Дек. 20, 2024

The purpose of this study was to examine the hepatic bacterial composition and metabolome characteristics patients with NAFLD using 16S rDNA sequencing metabolomics. results revealed substantial differences in metabolites between group control group. These were used identify potential biomarkers that could be employed diagnose NAFLD. Liver tissues from 13 12 collected for microbiota examination. DNA profiles liver significantly different controls. exhibited an enrichment Enterobacterales, Mycobacteriales, Pseudomonadales, Flavobacteriales Xanthomonadales, Sphingomonadales, Lysobact, which characterised by a lack erales. At genus level, abundance values Escherichia-Shigella, Rhodococcus, Chryseobacterium elevated, while Stenotrophomonas, Lawsonella Sphingobium reduced. A total 402 distinct identified two groups, 78 up-regulated 14 down-regulated. metabolic pathways indicated linoleic acid metabolism most significant contributor differences, lipid substantially differentiated. metabolite levels correlated changes microflora, as demonstrated correlation analysis. Differences pathogenesis host physiological function may attributed flora metabolomic characteristics. In future, presents new opportunities investigation prospective diagnostic therapeutic targets

Язык: Английский

Probiotic, Postbiotic, and Paraprobiotic Effects of Lactobacillus rhamnosus as a Modulator of Obesity-Associated Factors DOI Creative Commons
Gabriela López-Almada, María Esther Mejía-León, Norma Julieta Salazar‐López

и другие.

Foods, Год журнала: 2024, Номер 13(22), С. 3529 - 3529

Опубликована: Ноя. 5, 2024

Obesity is a pandemic currently affecting the world's population that decreases quality of life and promotes development chronic non-communicable diseases.

Язык: Английский

Процитировано

4

Microbiome metabolic capacity is buffered against phylotype losses by functional redundancy DOI Creative Commons

Kayla Cross,

Noelle G. Beckman,

Benjamin C. Jahnes

и другие.

Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 30, 2025

ABSTRACT Many animals contain a species-rich and diverse gut microbiota that likely contributes to several host-supportive services include diet processing nutrient provisioning. Loss of microbiome taxa their associated metabolic functions as result perturbations may in loss microbiome-level reduction capacity. If are shared by multiple (i.e., functional redundancy), including deeply divergent lineages, then the impact taxon/function losses be dampened. We examined what degree alterations phylotype diversity Feeding two nutritionally imbalanced diets omnivorous Periplaneta americana over 8 weeks reduced phylotype-rich microbiomes ~25% based on 16S rRNA gene amplicon sequencing, yet PICRUSt2-inferred pathway richness was largely unaffected due being polyphyletic. concluded nonlinearity between taxon is members sharing many well-characterized functions, with lineages remaining after perturbation potentially capable preventing “service outages” redundancy. IMPORTANCE Diet can affect taxonomic composition diversity, but its impacts community-level capabilities less clear. Host health fitness increasingly linked further modeling relationship capability needed inform these linkages. Invertebrate animal models like American cockroach ideal for this inquiry because they amenable various provide high replicates per treatment at low costs thus enabling rigorous statistical analyses hypothesis testing. Microbiome diet-labile feeding unbalanced post-treatment), predicted capacities post-treatment were affected resilience abundant surviving well taxa. These results suggest both profiles should considered when attempting infer how altering possible host outcomes.

Язык: Английский

Процитировано

0

Impact of obesity on infarct size, circulating biomarkers, mitochondrial function and mortality in a Göttingen minipig myocardial infarct model DOI Creative Commons
Mette Flethøj, Karina Poulsdóttir Debes,

Cecilie Larsen

и другие.

Lab Animal, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Obesity is a risk factor for the development of coronary artery disease and myocardial infarction (MI). However, most large animal studies MI are performed in lean animals. Here we assessed impact obesity on echocardiographic findings, infarct size, circulating biomarkers, mitochondrial respiratory capacity mortality closed-chest minipig model MI. The initial study population consisted 20 obese (median 60.0 kg [interquartile range 55.9-64.6 kg]) 18 (25.0 [23.4-36.5 female Göttingen minipigs. duration induction using western-style diet was up to approximately 6 months (156 days [24-162 days]) before by 120-min balloon occlusion left anterior descending feasible 17 Mortality higher compared with animals (53% versus 12%), driven primarily refractory ventricular fibrillation during occlusion. Electrocardiographic findings showed longer QRS QT intervals more extensive ST-segment elevation Plasma concentrations pro-atrial natriuretic peptide, pro-C-type peptide cardiac troponin T were significantly lower Infarct size estimated 8 weeks after smaller (10% [9-11%]) (13% [13-15%]). Finally, mitochondrial-complex-I-linked overall animals; however, no group difference found intrinsic capacity.

Язык: Английский

Процитировано

0

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig DOI

Yasmin Eckstein,

Benedikt M. Kessler, Arne Hinrichs

и другие.

STAR Protocols, Год журнала: 2025, Номер 6(2), С. 103774 - 103774

Опубликована: Апрель 16, 2025

Язык: Английский

Процитировано

0

Dietary polyunsaturated fatty acids effect on cecal microbiome profile of maturing broiler chicken DOI Creative Commons

Vidya V. Jadhav,

Yewande O. Fasina, Scott H. Harrison

и другие.

Poultry Science, Год журнала: 2025, Номер unknown, С. 105167 - 105167

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Synergistic Interplay of Diet, Gut Microbiota, and Insulin Resistance: Unraveling the Molecular Nexus DOI
Rajesh Kanna Gopal,

Pitchaipillai Sankar Ganesh,

Naji Naseef Pathoor

и другие.

Molecular Nutrition & Food Research, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 16, 2024

Abstract This comprehensive review explores the intricate relationship between gut microbiota, diet, and insulin resistance, emphasizing novel roles of diet‐induced microbial changes in influencing metabolic health. It highlights how diet significantly influences microbiota composition, with different dietary patterns fostering diverse communities. These microbiome impact human metabolism by affecting inflammation, energy balance, sensitivity, particularly through metabolites like short‐chain fatty acids (SCFAs). Focusing key mediators endotoxemia systemic introduces personalized microbiome‐based therapeutic strategies, it also investigates effects components—fiber, polyphenols, lipids—on along protein intake amino acid metabolism. The study compares Western Mediterranean diets on microbiota‐insulin resistance axis. Therapeutic implications, including probiotics, fecal transplantation (FMT), diets, are discussed. Key findings reveal that high‐fat especially those rich saturated fats, contribute to dysbiosis increased intestinal permeability, while high‐fiber promote beneficial bacteria SCFAs. underscores future potential food interventions for preventing or managing resistance.

Язык: Английский

Процитировано

3

Effect of exclusive enteral nutrition on gut microbiota in Malaysian children with Crohn’s disease DOI Open Access

Linah Wetthasinghe,

Hien Fuh Ng, Kee Seang Chew

и другие.

IOP Conference Series Earth and Environmental Science, Год журнала: 2024, Номер 1408(1), С. 012003 - 012003

Опубликована: Окт. 1, 2024

Abstract Paediatric Crohn’s disease (pCD), once primarily observed in Western countries, is now experiencing a global increase incidence rates. Southeast Asian nations, including Malaysia, are witnessing gradual of pCD. In addition to clinical symptoms, children with (CD) also have mental and emotional impairments. Children CD experience gut microbiota dysbiosis. Exclusive enteral nutrition (EEN) the recommended first line treatment for mild-to-moderate pCD due its multifaceted disease-modulating effects, positive re-modulation diseased microbiota. However, there exists critical knowledge gap regarding understanding dynamics individuals treated EEN, particularly regions like Malaysia. This study aimed fill this void by investigating profile Malaysian CD, making it kind Using targeted 16S ribosomal DNA (16S rDNA) next-generation sequencing, identified dominated Fusobacterium nucleatum , Haemophilus parainfluenzae mortiferum Acidaminococcus Monoglobus among patients pCD, latter three being newly relation Following successful EEN treatment, enrichment was bacterial groups such as Lachnoclostridium Parabacteroides Eubacterium fissicatena group. The Clostridium sensu stricto 13 subterminale Acetivibrio ethanolgignens group, Robinsoniella peoriensis which not been previously associated were found be increased following treatment. While some similarities previous literature noted, Malaysians exhibited several novel both during sheds light on interplay between pathology, emphasising versatility nutritional therapy approaches diverse populations. Further research area holds promise refining strategies improving outcomes individuals, only Malaysia but globally.

Язык: Английский

Процитировано

0

Characterization of the hepatic flora and metabolome in nonalcoholic fatty liver disease DOI Creative Commons

Hua Jiang,

Hui Wang, Yisong Guo

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Дек. 20, 2024

The purpose of this study was to examine the hepatic bacterial composition and metabolome characteristics patients with NAFLD using 16S rDNA sequencing metabolomics. results revealed substantial differences in metabolites between group control group. These were used identify potential biomarkers that could be employed diagnose NAFLD. Liver tissues from 13 12 collected for microbiota examination. DNA profiles liver significantly different controls. exhibited an enrichment Enterobacterales, Mycobacteriales, Pseudomonadales, Flavobacteriales Xanthomonadales, Sphingomonadales, Lysobact, which characterised by a lack erales. At genus level, abundance values Escherichia-Shigella, Rhodococcus, Chryseobacterium elevated, while Stenotrophomonas, Lawsonella Sphingobium reduced. A total 402 distinct identified two groups, 78 up-regulated 14 down-regulated. metabolic pathways indicated linoleic acid metabolism most significant contributor differences, lipid substantially differentiated. metabolite levels correlated changes microflora, as demonstrated correlation analysis. Differences pathogenesis host physiological function may attributed flora metabolomic characteristics. In future, presents new opportunities investigation prospective diagnostic therapeutic targets

Язык: Английский

Процитировано

0