Virulence and tissue tropism of different epidemiologically significant SARS-CoV-2 variants for golden Syrian hamsters DOI Creative Commons
A. V. Gracheva, Andrey O. Drokov, D. I. Smirnova

и другие.

Journal of microbiology epidemiology immunobiology, Год журнала: 2024, Номер 101(4), С. 470 - 482

Опубликована: Сен. 9, 2024

Introduction. Animal models for SARS-CoV-2 infection, reproducing the clinical features of COVID-19 in humans, are important tools studying pathogenesis disease, transmission pathogen and indispensable testing antiviral drugs vaccines. The aim study was to assess virulence tissue tropism golden Syrian hamsters strains belonging different epidemiologically significant variants: Wuhan-like, Delta, Omicron BA.1.1 BA.5.2. Materials methods. Hamsters were intranasally infected with strains. Virulence assessed by comparing dynamics weight, viral load organs histopathological changes lungs uninfected animals. Results. Wuhan-like Dubrovka strain had greatest hamsters, which manifested development severe pneumonia a delay weight gain 14.6%, high virus content lungs, nasal passages brain — 6.2, 5,9 3.7 lg TCID50/ml homogenate, respectively. Presumably, it infection central nervous system that negatively affected general condition When viruses Delta variants, observed minor loss animals uninformative, so indicators such as lung histopathology, passages, heart other played decisive role assessing pathogenicity. A score assessment histopathology particular value severity pneumonia, since reduced subjectivity evaluating results histological examination provided semi-quantitative pathological process. Conclusion. Despite revealed lower variants compared ancestral Wuhan virus, this animal model retains its conducting preclinical trials drugs.

Язык: Английский

NK cells modulate in vivo control of SARS-CoV-2 replication and suppression of lung damage DOI Creative Commons
Harikrishnan Balachandran, Kyle Kroll,

Karen Terry

и другие.

PLoS Pathogens, Год журнала: 2024, Номер 20(8), С. e1012439 - e1012439

Опубликована: Авг. 12, 2024

Natural killer (NK) cells play a critical role in virus control. However, it has remained largely unclear whether NK cell mobilization SARS-CoV-2 infections is beneficial or pathologic. To address this deficit, we employed validated experimental depletion non-human primate (NHP) model with Delta variant B.1.617.2 challenge. Viral loads (VL), numbers, activation, proliferation, and functional measures were evaluated blood tissues. In non-depleted (control) animals, infection rapidly induced expansion, increased tissue trafficking associated VL. Strikingly, report that leads to higher VL, longer duration of viral shedding, significantly levels pro-inflammatory cytokines the lungs, overt lung damage. Overall, find first significant conclusive evidence for cell-mediated control replication disease pathology. These data indicate adjunct therapies could benefit from cell-targeted approaches.

Язык: Английский

Процитировано

1
Virulence and tissue tropism of different epidemiologically significant SARS-CoV-2 variants for golden Syrian hamsters DOI Creative Commons
A. V. Gracheva, Andrey O. Drokov, D. I. Smirnova

и другие.

Journal of microbiology epidemiology immunobiology, Год журнала: 2024, Номер 101(4), С. 470 - 482

Опубликована: Сен. 9, 2024

Introduction. Animal models for SARS-CoV-2 infection, reproducing the clinical features of COVID-19 in humans, are important tools studying pathogenesis disease, transmission pathogen and indispensable testing antiviral drugs vaccines. The aim study was to assess virulence tissue tropism golden Syrian hamsters strains belonging different epidemiologically significant variants: Wuhan-like, Delta, Omicron BA.1.1 BA.5.2. Materials methods. Hamsters were intranasally infected with strains. Virulence assessed by comparing dynamics weight, viral load organs histopathological changes lungs uninfected animals. Results. Wuhan-like Dubrovka strain had greatest hamsters, which manifested development severe pneumonia a delay weight gain 14.6%, high virus content lungs, nasal passages brain — 6.2, 5,9 3.7 lg TCID50/ml homogenate, respectively. Presumably, it infection central nervous system that negatively affected general condition When viruses Delta variants, observed minor loss animals uninformative, so indicators such as lung histopathology, passages, heart other played decisive role assessing pathogenicity. A score assessment histopathology particular value severity pneumonia, since reduced subjectivity evaluating results histological examination provided semi-quantitative pathological process. Conclusion. Despite revealed lower variants compared ancestral Wuhan virus, this animal model retains its conducting preclinical trials drugs.

Язык: Английский

Процитировано

0