Discovery of Mutated Oncodriver Genes associated with Glioblastoma Originated from Stem Cells of Subventricular Zone through the Whole Exome Sequence Profile Analysis, and Drug Repurposing DOI Creative Commons

Arnob Sarker,

Burhan Uddin,

Reaz Ahmmed

и другие.

Heliyon, Год журнала: 2025, Номер 11(2), С. e42052 - e42052

Опубликована: Янв. 1, 2025

Glioblastoma (GBM) is one of the most aggressive cancers due to its high mortality rate in spite intensive treatment. It may be happened because drug resistance against their typical receptors, since these receptor genes are often mutated by environmental stress. So identifying oncodriver which could used as potential target essential order develop effective new therapeutic drugs well better prognosis for GBM patients. In this study, we analyzed whole exome sequencing (WES) profiles NCBI database on and matched-normal (control) samples originated from astrocyte like neural stem cells (NSC) subventricular zone (SVZ) explore GBM-causing genes, SVZ considered origin development. We detected 16 genes. Then, filtering differential co-expression analysis based independent RNA-Seq CGGA revealed 10 dysregulated Following that, 3 significantly overexpressed (MTCH2, VWF, WDR89) were identified targets. Then molecular mechanisms development investigated three driver through gene ontology (GO), KEGG-pathways, Gene regulatory network (GRN) mutation analysis. Finally, guided top-ranked six agents (Irinotecan, Imatinib, etoposide, pazopanib, trametinib cabozanitinib) recommended docking study. Most our findings received support literature review also. Therefore, study might carry values wet-lab researchers further investigation terms diagnosis therapies GBM.

Язык: Английский

Discovery of Mutated Oncodriver Genes associated with Glioblastoma Originated from Stem Cells of Subventricular Zone through the Whole Exome Sequence Profile Analysis, and Drug Repurposing DOI Creative Commons

Arnob Sarker,

Burhan Uddin,

Reaz Ahmmed

и другие.

Heliyon, Год журнала: 2025, Номер 11(2), С. e42052 - e42052

Опубликована: Янв. 1, 2025

Glioblastoma (GBM) is one of the most aggressive cancers due to its high mortality rate in spite intensive treatment. It may be happened because drug resistance against their typical receptors, since these receptor genes are often mutated by environmental stress. So identifying oncodriver which could used as potential target essential order develop effective new therapeutic drugs well better prognosis for GBM patients. In this study, we analyzed whole exome sequencing (WES) profiles NCBI database on and matched-normal (control) samples originated from astrocyte like neural stem cells (NSC) subventricular zone (SVZ) explore GBM-causing genes, SVZ considered origin development. We detected 16 genes. Then, filtering differential co-expression analysis based independent RNA-Seq CGGA revealed 10 dysregulated Following that, 3 significantly overexpressed (MTCH2, VWF, WDR89) were identified targets. Then molecular mechanisms development investigated three driver through gene ontology (GO), KEGG-pathways, Gene regulatory network (GRN) mutation analysis. Finally, guided top-ranked six agents (Irinotecan, Imatinib, etoposide, pazopanib, trametinib cabozanitinib) recommended docking study. Most our findings received support literature review also. Therefore, study might carry values wet-lab researchers further investigation terms diagnosis therapies GBM.

Язык: Английский

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