Hyaluronic Acid-Based pH-Sensitive Nanogel: Synthesis, Characterization, and Assessment for Acyclovir Delivery In Vitro and In Vivo DOI
Tarek A. A. Moussa, Heba Ali Abd El‐Rahman,

Riham R. Mohamed

и другие.

Biomacromolecules, Год журнала: 2024, Номер 26(1), С. 341 - 362

Опубликована: Дек. 25, 2024

Acyclovir (ACV) is a potentially effective antiviral medication; however, it has serious drawback, which its poor solubility, bioavailability, and short half-life. The goal of this study to improve drawbacks through the synthesis nanogels. In study, cross-linked hyaluronic acid-grafted poly(acrylamide-co-itaconic acid) nanogel synthesized successfully free radical polymerization used as safe pH-responsive carrier for ACV. nanogels showed pH response in vitro vivo. prepared C5 (1:1 ratio acrylamide: itaconic), had highest grafting efficiency, maximum swelling, drug loading, release 7.4, higher than 1.2. Also, C5, large surface area, good stability, matrices shrank acidic medium protected drug, while basic medium, expanded released ACV sustained manner improved bioavailability half-life

Язык: Английский

Tamarind seed gum-based hydrogel for the targeted delivery of imidazobenzothiazole sulfonamide derivative as an anticancer agent DOI

Bhavya Balakrishnan,

B.K. Sarojini,

Bikrodi Sesappa Dayananda

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 295, С. 139665 - 139665

Опубликована: Янв. 8, 2025

Язык: Английский

Процитировано

1

Convenient Synthesis, Characterization, Evaluation and molecular docking of Some New Fused Pyrazolo[3,4-d]pyrimidine Derivatives and 3-Methyl-8 phenylpyrazolo[3',4':4,5]pyrimido[6,1-c][1,2,4]triazines Against Hela Cancer Cells DOI

Mirna T. Helmy,

Mohamed A. Mohamed Teleb, Demiana H. Hanna

и другие.

Tetrahedron, Год журнала: 2025, Номер unknown, С. 134557 - 134557

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Apoptosis induction in ascorbic acid treated human colorectal cancer cell lines (Caco-2) DOI

Naeimah Abdullah Mohammed Mustafa,

Mohamed A. El-Desouky,

Nessreen A. Shawush

и другие.

Journal of Biologically Active Products from Nature, Год журнала: 2025, Номер 15(1), С. 56 - 71

Опубликована: Янв. 2, 2025

Язык: Английский

Процитировано

0

Palladium‐Imidazole Nanoparticles' Cytotoxic Effects on Colon Cancer Cells: Induction of Cell Cycle Arrest and Apoptosis Mediated via Mitochondria DOI
Khaled M. Ismail, Safaa S. Hassan, Demiana H. Hanna

и другие.

Applied Organometallic Chemistry, Год журнала: 2024, Номер 39(1)

Опубликована: Дек. 10, 2024

ABSTRACT The present study emphasized the potential antitumor activity of compound A; [Pd (Im) 2 Cl ], a nano‐sized coordination with size 23.76 nm. Characterization was performed using elemental analysis, molar conductance, magnetic measurement, FTIR, Ultraviolet–visible (UV–vis) spectroscopy, thermal mass spectrometry, and NMR. complex showed stability up to 300°C. Furthermore, compound's formation spontaneity supported by activation parameters calculated from TGA. di‐aqua form separated solid complex, B (H O) ] 2+ , successfully prepared. geometry optimization imidazole its complexes that both were more stable than free imidazole. cis exhibits superior potent IC 50 value 5.72±0.68 μg/mL. This demonstrates greater inhibitory impacts on Caco‐2 cancer cell proliferation compared ]. Moreover, cytoxicity against cells confirmed significant increase in LDH levels treated untreated ones. Treatment markedly raised percentage early late apoptotic cells, accompanied Go/G1 phase arrest intense comet nucleus. Apoptosis induction mediated through increased reactive oxygen species (ROS) production. expression cleaved Caspase‐3, Bax, P53 while concurrently dropping Bcl‐2, cyclin D1, CDK4. Overall, these findings indicate triggers cytotoxicity dose‐dependent manner, primarily via ROS‐mediated death, possibly mitochondrial pathway. Additionally, active selected proteins related apoptosis cycle explored theoretical molecular docking results. outcomes also validated safety healthy suggesting it as promising treatment option for colon humans.

Язык: Английский

Процитировано

4

Berberine decorated zinc oxide loaded chitosan nanoparticles a potent anti cancer agent against breast cancer DOI Creative Commons

Fatemeh Esnaashari,

Hojjatolah Zamani, Hossein Zahmatkesh

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 25, 2025

Breast cancer ranks as the second leading reason of mortality among females globally, emphasizing critical need for novel anticancer treatments. In current work, berberine-zinc oxide conjugated chitosan nanoparticles were synthesized and characterized using various characterization techniques. The cytotoxic effects CS-ZnO-Ber NPs on MCF-7 cells assessed MTT assay. Also, annexin V-FITC/PI double staining, Hoechst 33,342 caspases-8 9 activity assays, cell cycle analysis performed. Furthermore, mRNA levels Bax Bcl-2 genes quantified qPCR. Additionally, migration was evaluated a scratch IC50 value against measured 7.41 µg/mL. Apoptosis induction in NP-treated confirmed by Annexin V/PI accompanied observation condensed chromatin fragmented DNA. Moreover, pro-apoptotic potential evidenced significant increases decreased Bcl-2/Bax ratio. arrest at sub-G1 noticed treated cells. markedly inhibited rate These findings suggest that induce cell-cycle activate apoptotic pathways cells, highlighting their hopeful therapeutic agent breast cancer.

Язык: Английский

Процитировано

0

Targeted delivery of Zingiber officinale Roscoe extract-loaded chitosan nanoparticles for inhibition of multidrug-resistant Escherichia coli-induced HCT-116 colon cancer DOI Creative Commons
Cletus Anes Ukwubile, Troy Salvia Malgwi,

Collins Emeka Odu

и другие.

Clinical Phytoscience, Год журнала: 2025, Номер 11(1)

Опубликована: Март 27, 2025

Abstract The emergence of multidrug-resistant ( MDR ) Escherichia coli E. is a growing concern, as it significantly contributes to colon cancer initiation and progression. Conventional treatment strategies face challenges such poor bioavailability antibiotic resistance, necessitating the development targeted therapies. This study evaluates efficacy Zingiber officinale Roscoe (ginger) extract-loaded chitosan nanoparticles GELCs in inhibiting -induced using HCT-116 cell line. were synthesized with an average size 150 ± 5 nm, zeta potential + 32.5 mV, encapsulation efficiency 84.3%. Their biological activity was assessed through vitro vivo models, focusing on antimicrobial effects, cytotoxicity, apoptosis induction, inflammatory modulation. exhibited potent activity, reducing colony-forming units (CFUs) by 83% compared untreated co-cultures. MTT assay results showed dose-dependent reduction viability, IC₅₀ 22.8 µg/mL , which lower than that free ginger extract (IC₅₀ = 48.6 µg/mL), indicating enhanced efficacy. Flow cytometry analysis confirmed increased apoptosis, BAX/BCl-2 ratio 11.3 GELC -treated group 1.0 control . ELISA assays further demonstrated cytokines, IL-6, TNF-α, IL-1β levels decreasing 54%, 61%, 47% respectively. These findings highlight dual anticancer making them promising candidate for therapy against -associated cancer. underscores advantages nanoparticle-based delivery systems enhancing therapeutic natural bioactive compounds.

Язык: Английский

Процитировано

0

Hyaluronic Acid-Based pH-Sensitive Nanogel: Synthesis, Characterization, and Assessment for Acyclovir Delivery In Vitro and In Vivo DOI
Tarek A. A. Moussa, Heba Ali Abd El‐Rahman,

Riham R. Mohamed

и другие.

Biomacromolecules, Год журнала: 2024, Номер 26(1), С. 341 - 362

Опубликована: Дек. 25, 2024

Acyclovir (ACV) is a potentially effective antiviral medication; however, it has serious drawback, which its poor solubility, bioavailability, and short half-life. The goal of this study to improve drawbacks through the synthesis nanogels. In study, cross-linked hyaluronic acid-grafted poly(acrylamide-co-itaconic acid) nanogel synthesized successfully free radical polymerization used as safe pH-responsive carrier for ACV. nanogels showed pH response in vitro vivo. prepared C5 (1:1 ratio acrylamide: itaconic), had highest grafting efficiency, maximum swelling, drug loading, release 7.4, higher than 1.2. Also, C5, large surface area, good stability, matrices shrank acidic medium protected drug, while basic medium, expanded released ACV sustained manner improved bioavailability half-life

Язык: Английский

Процитировано

3