Phage cocktail amikacin combination as a potential therapy for bacteremia associated with carbapenemase producing colistin resistant Klebsiella pneumoniae DOI Creative Commons

Aye Mya Sithu Shein,

Dhammika Leshan Wannigama, Cameron Hurst

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 22, 2024

The increasing occurrence of hospital-associated infections, particularly bacteremia, caused by extensively drug-resistant (XDR) carbapenemase-producing colistin-resistant Klebsiella pneumoniae highlights a critical requirement to discover new therapeutic alternatives. Bacteriophages having host-specific bacteriolytic effects are promising alternatives for combating these pathogens. Among 12 phages isolated from public wastewater in Thailand, two phages-vB_kpnM_05 (myovirus) and vB_kpnP_08 (podovirus) showed broad-host range, producing activities against 81.3% (n = 26) 78.1% 25) 32 XDR K. pneumoniae, with capsular types—K15, K17, K50, K51, K52/wzi-50 K2/wzi-2. Both short replication times, large burst sizes rapid adsorptions. They exhibited significant stability under various environmental conditions. Genomic analysis revealed that both genetically distinct Myoviridae Podoviridae family, the lack toxin, virulence, lysogeny antibiotic resistance genes. These characteristics highlighted their potential utilizing phage therapy pneumoniae. Although cocktail combining vB_kpnM_05 provided bacteriolysis longer duration (8 h) than its monophage (6 h), bacterial regrowth was observed which suggested an evitable development phages' selection pressures. Future study will be undertaken elucidate precise mechanisms developed associated fitness cost. Remarkably, amikacin at sub-inhibitory concentrations produced potent synergy completely suppressing vitro. Our demonstrated prophylactic effectiveness cocktail-amikacin combination as alternative strategy overcoming bacteremia carbapenemase colistin vivo.

Язык: Английский

Lactiplantibacillus plantarum BXM2 Treatment Alleviates Disorders Induced by a High-Fat Diet in Mice by Improving Intestinal Health and Modulating the Gut Microbiota DOI Open Access

Xiao-hui Cai,

Juqing Huang,

Tian Yu

и другие.

Nutrients, Год журнала: 2025, Номер 17(3), С. 407 - 407

Опубликована: Янв. 23, 2025

Objective: Lactiplantibacillus plantarum BXM2 is a novel probiotic derived from fermented passion fruit (Passiflora edulis) juice that possesses promising potential. The aim of this study was to evaluate the beneficial effects L. supplementation in mice. Methods: orally administered male SPF C57BL/6J mice fed high-fat diet (HFD) its anti-obesity potential, as well on intestinal health and microbiota. Results: Our results demonstrated significantly decreased perirenal adipose index improved by increasing ratio villus height crypt depth goblet cell number intestine. Furthermore, treatment exhibited regulatory chronic inflammation normalizing mRNA expression TNF-α IL-6. Of note, reversed HFD-induced gut dysbiosis, evidenced Bacillota (Firmicutes) Bacteroidota, abundance obesity-related genera Dubosiella, Romboutsia, Lachnospiraceae_UCG006, increased Akkermansia Lactobacillus. Conclusions: findings support role interventions targeting dysbiosis disorders.

Язык: Английский

Процитировано

0
Phage cocktail amikacin combination as a potential therapy for bacteremia associated with carbapenemase producing colistin resistant Klebsiella pneumoniae DOI Creative Commons

Aye Mya Sithu Shein,

Dhammika Leshan Wannigama, Cameron Hurst

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 22, 2024

The increasing occurrence of hospital-associated infections, particularly bacteremia, caused by extensively drug-resistant (XDR) carbapenemase-producing colistin-resistant Klebsiella pneumoniae highlights a critical requirement to discover new therapeutic alternatives. Bacteriophages having host-specific bacteriolytic effects are promising alternatives for combating these pathogens. Among 12 phages isolated from public wastewater in Thailand, two phages-vB_kpnM_05 (myovirus) and vB_kpnP_08 (podovirus) showed broad-host range, producing activities against 81.3% (n = 26) 78.1% 25) 32 XDR K. pneumoniae, with capsular types—K15, K17, K50, K51, K52/wzi-50 K2/wzi-2. Both short replication times, large burst sizes rapid adsorptions. They exhibited significant stability under various environmental conditions. Genomic analysis revealed that both genetically distinct Myoviridae Podoviridae family, the lack toxin, virulence, lysogeny antibiotic resistance genes. These characteristics highlighted their potential utilizing phage therapy pneumoniae. Although cocktail combining vB_kpnM_05 provided bacteriolysis longer duration (8 h) than its monophage (6 h), bacterial regrowth was observed which suggested an evitable development phages' selection pressures. Future study will be undertaken elucidate precise mechanisms developed associated fitness cost. Remarkably, amikacin at sub-inhibitory concentrations produced potent synergy completely suppressing vitro. Our demonstrated prophylactic effectiveness cocktail-amikacin combination as alternative strategy overcoming bacteremia carbapenemase colistin vivo.

Язык: Английский

Процитировано

2