A polymeric, PDMS-based large-scale skull replacement suitable for optical and mechanical access for long-term neuronal imaging, electrophysiology, and optogenetics DOI Creative Commons
Kengo Takahashi, Gerjan Huis in ’t Veld, Davide Benedetti

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

Summary Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain, such as widefield two-photon calcium imaging, electrophysiology, optogenetics, are now available. However, few effective approaches enable both optical mechanical access brain. In this work, we offer an in-depth guide for synthesizing, implanting, using polydimethylsiloxane (PDMS) windows skull replacements chronic imaging. Furthermore, provide instructions perform viral injections multi-site silicon probe implantation. Graphical abstract

Язык: Английский

A polymeric, PDMS-based large-scale skull replacement suitable for optical and mechanical access for long-term neuronal imaging, electrophysiology, and optogenetics DOI Creative Commons
Kengo Takahashi, Gerjan Huis in ’t Veld, Davide Benedetti

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

Summary Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain, such as widefield two-photon calcium imaging, electrophysiology, optogenetics, are now available. However, few effective approaches enable both optical mechanical access brain. In this work, we offer an in-depth guide for synthesizing, implanting, using polydimethylsiloxane (PDMS) windows skull replacements chronic imaging. Furthermore, provide instructions perform viral injections multi-site silicon probe implantation. Graphical abstract

Язык: Английский

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