bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 4, 2023
ABSTRACT
We
previously
performed
a
genome-wide
association
study
(GWAS)
to
identify
the
genetic
basis
of
praziquantel
(PZQ)
response
in
schistosomes,
identifying
two
quantitative
trait
loci
(QTL)
situated
on
chromosome
2
and
3.
reanalyzed
this
GWAS
using
latest
(v10)
genome
assembly
showing
that
single
locus
3,
rather
than
independent
loci,
determines
drug
response.
These
results
reveal
is
monogenic
demonstrates
importance
high-quality
genomic
information.
PLoS Pathogens,
Год журнала:
2022,
Номер
18(8), С. e1010706 - e1010706
Опубликована: Авг. 8, 2022
Hybridization
between
different
species
of
parasites
is
increasingly
being
recognised
as
a
major
public
and
veterinary
health
concern
at
the
interface
infectious
diseases
biology,
evolution,
epidemiology
ultimately
control.
Recent
research
has
revealed
that
viable
hybrids
introgressed
lineages
Schistosoma
spp.
are
prevalent
across
Africa
beyond,
including
those
with
zoonotic
potential.
However,
it
remains
unclear
whether
these
hybrid
represent
recent
hybridization
events,
suggesting
ongoing,
and/or
they
derived
from
ancient
events.
In
human
schistosomiasis,
investigation
hampered
by
inaccessibility
adult-stage
worms
due
to
their
intravascular
location,
an
issue
which
can
be
circumvented
post-mortem
livestock
abattoirs
for
known
To
characterise
composition
naturally-occurring
schistosome
hybrids,
we
performed
whole-genome
sequencing
21
natural
infective
isolates.
facilitate
this,
also
assembled
de
novo
chromosomal-scale
draft
assembly
curassoni
.
Genomic
analyses
identified
isolates
S
bovis
,
two
species,
all
were
early
generation
multiple
generations
found
within
same
host.
These
results
show
ongoing
process
populations
potential
further
challenge
elimination
efforts
against
schistosomiasis.
Antimicrobial Agents and Chemotherapy,
Год журнала:
2024,
Номер
68(3)
Опубликована: Янв. 30, 2024
We
previously
performed
a
genome-wide
association
study
(GWAS)
to
identify
the
genetic
basis
of
praziquantel
(PZQ)
response
in
schistosomes,
identifying
two
quantitative
trait
loci
situated
on
chromosomes
2
and
3.
reanalyzed
this
GWAS
using
latest
(version
10)
genome
assembly
showing
that
single
locus
chromosome
3,
rather
than
independent
loci,
determines
drug
response.
These
results
reveal
PZQ
is
monogenic
demonstrates
importance
high-quality
genomic
information.
PLoS neglected tropical diseases,
Год журнала:
2024,
Номер
18(7), С. e0012282 - e0012282
Опубликована: Июль 11, 2024
Overview
The
roadmap
adopted
by
the
World
Health
Organization
(WHO)
for
eliminating
neglected
tropical
diseases
aims
to
eliminate
schistosomiasis,
as
a
public
health
concern,
2030.
While
progress
has
been
made
towards
reducing
schistosomiasis
morbidity
control
in
several
sub-Saharan
African
countries,
there
is
still
more
that
needs
be
done.
Proper
surveillance
using
accurate
diagnostics
with
acceptable
sensitivity
and
specificity
essential
evaluating
success
of
all
efforts
against
schistosomiasis.
Microscopy,
despite
its
low
sensitivity,
remains
gold
standard
approach
diagnosing
disease.
Although
many
have
develop
new
based
on
circulating
parasite
proteins,
genetic
markers,
schistosome
egg
morphology,
their
paramagnetic
properties,
none
robust
enough
replace
microscopy.
This
review
highlights
common
diagnostic
approaches
detecting
field
clinical
settings,
major
challenges,
provides
novel
opportunities
diagnosis
pathways
will
critical
supporting
elimination
Methods
We
searched
relevant
reliable
published
literature
from
PubMed,
Scopus,
google
scholar,
Web
science.
search
strategies
were
primarily
determined
subtopic,
hence
following
words
used
(schistosom*,
diagnosis,
Kato–Katz,
antibody
test,
antigen,
POC-CCA,
UCP-LF-CAA,
molecular
diagnostics,
nucleic
acid
amplification
microfluidics,
lab-on
disk,
chip,
recombinase
polymerase
(RPA),
LAMP,
portable
sequencer,
nanobody
identical
multi-repeat
sequences,
TPPs,
REASSURED,
extraction
free),
Boolean
operators
AND
and/OR
refine
searching
capacity.
Due
global
nature
we
also
documents,
reports,
research
papers
international
organizations,
(WHO),
Center
Disease
Elimination.
PLoS neglected tropical diseases,
Год журнала:
2024,
Номер
18(7), С. e0012267 - e0012267
Опубликована: Июль 2, 2024
When
two
species
hybridize,
the
parental
genomes
are
brought
together
and
some
alleles
might
interact
for
first
time.
To
date,
extent
of
transcriptomic
changes
in
hybrid
generations,
along
with
their
functional
outcome
constitute
an
important
knowledge
gap,
especially
parasite
species.
Here
we
explored
molecular
outcomes
hybridization
first-generation
hybrids
between
blood
fluke
parasites
Schistosoma
haematobium
S.
bovis.
Through
a
approach,
measured
gene
expression
both
hybrids.
We
described
quantified
profiles
encountered
main
biological
processes
impacted.
Up
to
7,100
genes
fell
into
particular
profile
(intermediate
levels,
over-expressed,
under-expressed,
or
expressed
like
one
lines).
Most
these
were
different
depending
on
direction
cross
(S.
bovis
mother
father
reverse)
sex.
For
given
sex
direction,
vast
majority
hence
unassigned
profile:
either
they
differentially
but
not
typical
any
neither
lines
nor
lines.
The
most
prevalent
was
intermediate
(24%
investigated
genes).
These
results
suggest
that
compatibility
remains
quite
high.
also
found
support
over-dominance
model
(over-
under-expressed
compared
lines)
potentially
associated
heterosis.
In
females
particular,
such
as
reproductive
processes,
metabolism
cell
interactions
well
signaling
pathways
indeed
affected.
Our
study
provides
new
insight
biology
evidences
supporting
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2784 - 2784
Опубликована: Март 19, 2025
Alveolar
echinococcosis
(AE)
in
humans
is
caused
by
the
larval
(metacestode)
stage
of
Echinococcus
multilocularis,
commonly
known
as
‘fox
tapeworm’.
This
disease
predominantly
targets
liver
and
has
an
invasive
growth
pattern,
allowing
it
to
spread
adjacent
distant
tissues.
Due
its
gradual
progression
tumour-like
characteristics,
early
diagnosis
prompt
intervention
are
crucial,
particularly
there
currently
no
highly
effective
vaccines
or
chemotherapeutics
against
AE.
Current
estimates
suggest
that
~10,500
new
infections
occur
annually
worldwide;
however,
more
research
required
refine
prevalence
incidence
data
for
both
human
animal
hosts
endemic
areas
world.
article
discusses
biology
E.
outlines
aspects
pathogenesis,
diagnosis,
treatment,
management
AE,
reviews
global
distribution,
annual
incidence,
prevalence,
highlights
role
molecular
parasitology
advancing
therapeutic
strategies,
presents
recommendations
improving
prevention
control
AE
populations.
Infectious Diseases of Poverty,
Год журнала:
2025,
Номер
14(1)
Опубликована: Апрель 18, 2025
Abstract
Background
The
Schistosomiasis
Collection
at
the
Natural
History
Museum
(SCAN)
is
a
repository
of
schistosomiasis-related
specimens,
development
which
was
funded
by
Wellcome
Trust
between
2011
and
2021.
With
view
to
facilitating
research
improving
access
genetically
diverse
material,
SCAN
built
from
legacy
collections
specimens
amassed
over
decades,
with
more
recent
made
through
partnership
large
field-based
projects.
Methods
We
identified
literature
associated
2012
until
2024,
using
both
database
searches
(search
terms:
SCAN,
schistosomiasis
collection
NHM
schistosomiasis)
citations
publication
originally
laid
out
scope
Collection.
Studies
were
included
if
cited,
and/or
utilised
in
work.
Data
extracted
year
publication,
authors,
whether
how
used
work,
type
used.
Results
includes
88
published
works,
demonstrating
utility
supporting
research.
comprises
around
half
million
larval
schistosomes
originating
field,
approximately
3000
specimen
lots
lab-passaged
adult
parasites
stored
liquid
nitrogen.
11
schistosome
species,
majority
being
human
pathogens
Schistosoma
haematobium
S.
mansoni
,
while
also
including
many
livestock-associated
species.
Genome
analysis
S
.
guineensis
samples
indicate
historical
introgression
or
ongoing
hybridisation.
In
order
representation,
(>
19,000
forms
eggs,
550
laboratory
passaged
worms),
japonicum
bovis
curassoni
mattheei
rodhaini
intercalatum
margrebowiei
spindale
represented
only
laboratory-passaged
isolates
210,000
snails,
as
whole
encompassing
27
countries.
Conclusions
Improvements
DNA
sequencing
techniques
have
allowed
genome-level
data
be
accessed
archived
retrospective
collected
decades
ago.
has
been
use
exploring
diversity,
particularly
reference
hybridisation
drug
resistance.
Multiple
author
nationalities
demonstrate
collaborative
nature
Collection,
although
may
need
done
future,
promote
work
led
developing
countries
ensure
effective
collaboration
sample
sharing.
Graphical
Parasites & Vectors,
Год журнала:
2024,
Номер
17(1)
Опубликована: Май 8, 2024
Schistosomiasis
is
a
neglected
tropical
disease
that
afflicts
millions
of
people
worldwide;
it
caused
by
Schistosoma,
the
only
dioecious
flukes
with
ZW
systems.
Schistosoma
japonicum
endemic
to
Asia;
Z
chromosome
S.
comprises
one-quarter
entire
genome.
Detection
positive
selection
using
resequencing
data
understand
adaptive
evolution
has
been
applied
variety
pathogens,
including
japonicum.
However,
contribution
and
adaptation
often
neglected.
We
obtained
1,077,526
high-quality
SNPs
on
in
72
re-sequencing
publicly.
To
examine
faster
effect,
we
compared
sequence
divergence
two
closely
related
species,
haematobium
mansoni.
Genetic
diversity
was
between
autosomes
calculating
nucleotide
(π)
Dxy
values.
Population
structure
also
assessed
based
PCA
analysis.
Besides,
employed
multiple
methods
Tajima's
D,
FST,
iHS,
XP-EHH,
CMS
detect
signals
chromosome.
Further
RNAi
knockdown
experiments
were
performed
investigate
potential
biological
functions
candidate
genes.
Our
study
found
showed
more
pronounced
genetic
than
autosomes,
although
effect
may
be
smaller
variation
among
Compared
had
sub-populations.
Notably,
identified
set
genes
associated
host-parasite
co-evolution.
In
particular,
LCAT
exhibited
significant
within
Taiwan
population.
RNA
interference
suggested
necessary
for
survival
propagation
definitive
host.
addition,
several
specificity
intermediate
host
C-M
population,
Rab6
VCP,
which
are
involved
immune
evasion
provides
valuable
insights
into
further
advances
our
understanding
co-evolution
this
medically
important
parasite
its
hosts.
Infection Genetics and Evolution,
Год журнала:
2024,
Номер
122, С. 105609 - 105609
Опубликована: Май 26, 2024
Nuclear
hormone
receptors
(NHRs)
are
emerging
target
candidates
against
nematode
infection
and
resistance.
However,
there
is
a
lack
of
comprehensive
information
on
NHR-coding
genes
in
parasitic
nematodes.
In
this
study,
we
curated
the
nhr
gene
family
for
60
major
nematodes
from
humans
animals.
Compared
with
free-living
model
organism
Caenorhabditis
elegans,
remarkable
contraction
was
revealed
species,
genetic
diversification
conservation
unveiled
among
Clades
I
(10-13),
III
(16-42),
IV
(33-35)
V
(25-64).
Using
an
vitro
biosystem,
demonstrated
that
40
blood-feeding
Haemonchus
contortus
(clade
V;
barber's
pole
worm)
were
responsive
to
host
serum
one
(i.e.,
nhr-64)
consistently
stimulated
by
anthelmintics
ivermectin,
thiabendazole
levamisole);
high-throughput
RNA
interference
platform,
knocked
down
43
H.
identified
at
least
two
required
viability
nhr-105)
development
nhr-17)
infective
larvae
vitro.
Harnessing
preliminary
functional
atlas
will
prime
biological
studies
genetics,
infection,
anthelmintic
metabolism
within
animals,
as
well
promising
discovery
novel
intervention
targets.