A case study ofWhirly1(WHY1) evolution in the angiosperms: altered sequence, splicing, and expression in a clade of early-transitional mycoheterotrophic orchids DOI Creative Commons

Rachel M. Muti,

Craig F. Barrett, Brandon T. Sinn

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 24, 2023

Abstract The plastid-targeted transcription factor Whirly1 ( WHY1 ) has been implicated in chloroplast biogenesis, plastid genome stability, and fungal defense response, which together represent characteristics of interest for the study autotrophic losses across angiosperms. While gene loss nuclear genomes well studied mycoheterotrophic plants, evolution molecular mechanisms impacting stability are completely unknown. Here we characterize four early-transitional orchid species genus Corallorhiza by synthesizing results phylogenetic, transcriptomic, comparative genomic analyses with sequences sampled from 21 orders We found an increased number non-canonical isoforms assembled all but greenest species, including intron retention some isoforms. Within , phylotranscriptomic revealed presence tissue-specific differential expression only most photosynthetically capable a coincident increase fully species. Gene- codon-level tests selective regimes did not infer significant signal either relaxed selection or episodic diversifying so late-stage full orchids Epipogium aphyllum Gastrodia elata . Taken together, this provides comprehensive view angiosperms to date. Our reveal that splicing alteration decreased deceased plastome group these changes may precede shifts observed

Язык: Английский

Establishment of efficient Trichosanthes mottle mosaic virus-derived gene silencing in cucurbit plants DOI Creative Commons
Cheng Chen, Fang Zhu, Min Du

и другие.

Stress Biology, Год журнала: 2025, Номер 5(1)

Опубликована: Май 20, 2025

Abstract The Cucurbitaceae family includes a wide range of economically important fruits and vegetables; however, the laborious highly inefficient genetic transformation efficacy cucurbits has hindered exploration their gene functions. Virus-induced silencing (VIGS) technology, employed from antiviral RNA defense, emerged as viable alternative for high-throughput study plant function. In this study, we successfully established VIGS system utilizing Trichosanthes mottle mosaic virus (TrMMV), new member genus Tobamovirus . We demonstrated high durability mediated by TrMMV-VIGS vector in Nicotiana benthamiana , well several cucurbit species, including Cucurbita pepo Cucumis sativus C. lanatus melo insertion 90–400 bp fragments into led to effective target both with notably higher efficiency observed Furthermore, induced pronounced photobleaching phenotype flowers underscoring its potential application functional genomic research concerning floral traits particular species. Taken together, developed herein will facilitate rapid identification functions crops.

Язык: Английский

Процитировано

0
Inhibition of pepper huasteco yellow veins virus by foliar application of ZnO nanoparticles in Capsicum annuum L DOI
Samantha de Jesús Rivero-Montejo, Rafael F. Rivera-Bustamante,

Diana L. Saavedra-Trejo

и другие.

Plant Physiology and Biochemistry, Год журнала: 2023, Номер 203, С. 108074 - 108074

Опубликована: Окт. 1, 2023

Язык: Английский

Процитировано

6
Different viral effectors hijack TCP17, a key transcription factor for host Auxin synthesis, to promote viral infection DOI Creative Commons

Yanxiao Zhao,

Yong He, Xinyue Chen

и другие.

PLoS Pathogens, Год журнала: 2024, Номер 20(8), С. e1012510 - e1012510

Опубликована: Авг. 29, 2024

Auxin is an important class of plant hormones that play role in growth development, biotic stress response, and viruses often suppress host auxin levels to promote infection. However, previous research on auxin-mediated disease resistance has focused mainly signaling pathway, the molecular mechanisms how pathogenic proteins manipulate biosynthetic pathway remain poorly understood. TCP a plant-specific transcription factors, which TCP17 member binds promoter YUCCAs , key rate-limiting enzyme for synthesis, promotes expression involved synthesis plants. In this study, we reported Tomato spotted wilt virus (TSWV) infection suppressed through its interaction with TCP17. Further studies revealed NSs protein encoded by TSWV disrupts dimerization TCP17, thereby inhibit transcriptional activation ability reducing content Consequently, interference inhibits response signal Transgenic plants overexpressing exhibit against infection, whereas knocking out were more susceptible Additionally, other RNA (BSMV, RSV TBSV) can also interact interfere dimerization. Notably, overexpression enhanced BSMV. This suggests plays crucial defense different types use viral target component

Язык: Английский

Процитировано

1
Comparative transcriptome analysis reveals key pathways and regulatory networks in early resistance of Glycine max to soybean mosaic virus DOI Creative Commons

Han Li,

Jinyang Liu, Xingxing Yuan

и другие.

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Окт. 19, 2023

As a high-value oilseed crop, soybean [ Glycine max (L.) Merr.] is limited by various biotic stresses during its growth and development. Soybean mosaic virus (SMV) devastating viral infection of that primarily affects young leaves causes significant production economic losses; however, the synergistic molecular mechanisms underlying response to SMV are largely unknown. Therefore, we performed RNA sequencing on SMV-infected resistant susceptible lines determine mechanism resistance SMV. When clean reads were aligned G. reference genome, total 36,260 genes identified as expressed used for further research. Most differentially (DEGs) associated with found be enriched in plant hormone signal transduction circadian rhythm according Kyoto Encyclopedia Genes Genomes analysis. In addition salicylic acid jasmonic acid, which well known disease resistance, abscisic indole-3-acetic cytokinin also involved immune soybean. Ca 2+ signaling related DEGs plant-pathogen interaction negatively influence resistance. Furthermore, MAPK cascade was either or responses SMV, depending different downstream proteins. The phytochrome interacting factor-cryptochrome-R protein module MEKK3/MKK9/MPK7-WRKY33-CML/CDPK play essential roles based protein-protein prediction. Our findings provide general insights into regulatory networks have potential improve legume infection.

Язык: Английский

Процитировано

2
Development and application of monoclonal antibody-based dot-ELISA and colloidal gold immunochromatographic strip for rapid, specific, and sensitive detection of tomato brown rugose fruit virus DOI

Xinru Zhao,

Jiayu Wu,

Ziyue Ma

и другие.

Journal of Virological Methods, Год журнала: 2023, Номер 323, С. 114841 - 114841

Опубликована: Ноя. 7, 2023

Язык: Английский

Процитировано

2
Ethylene Response Transcription Factor 5 (ERF5) Enhances Defense Against Tobacco Curly Shoot Virus and Associated Betasatellite (TbCSV/TbCSB) in Nicotiana benthamiana DOI
Meisheng Zhao, Liping Zhang, Hussein Ghanem

и другие.

Virology, Год журнала: 2024, Номер 603, С. 110309 - 110309

Опубликована: Ноя. 29, 2024

Язык: Английский

Процитировано

0
A case study ofWhirly1(WHY1) evolution in the angiosperms: altered sequence, splicing, and expression in a clade of early-transitional mycoheterotrophic orchids DOI Creative Commons

Rachel M. Muti,

Craig F. Barrett, Brandon T. Sinn

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 24, 2023

Abstract The plastid-targeted transcription factor Whirly1 ( WHY1 ) has been implicated in chloroplast biogenesis, plastid genome stability, and fungal defense response, which together represent characteristics of interest for the study autotrophic losses across angiosperms. While gene loss nuclear genomes well studied mycoheterotrophic plants, evolution molecular mechanisms impacting stability are completely unknown. Here we characterize four early-transitional orchid species genus Corallorhiza by synthesizing results phylogenetic, transcriptomic, comparative genomic analyses with sequences sampled from 21 orders We found an increased number non-canonical isoforms assembled all but greenest species, including intron retention some isoforms. Within , phylotranscriptomic revealed presence tissue-specific differential expression only most photosynthetically capable a coincident increase fully species. Gene- codon-level tests selective regimes did not infer significant signal either relaxed selection or episodic diversifying so late-stage full orchids Epipogium aphyllum Gastrodia elata . Taken together, this provides comprehensive view angiosperms to date. Our reveal that splicing alteration decreased deceased plastome group these changes may precede shifts observed

Язык: Английский

Процитировано

0