The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer DOI Creative Commons
M. I. Khalil, Canchai Yang, Lexi Vu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 4, 2023

The human papillomavirus (HPV) oncoprotein E7 is a relatively short-lived protein required for HPV-driven cancer development and maintenance. degraded through ubiquitination mediated by cullin 1 (CUL1) the ubiquitin-conjugating enzyme E2 L3 (UBE2L3). However, proteins are maintained at high levels in most HPV-positive cells. A previous proteomics study has shown that UBE2L3 CUL1 increased knockdown of E3 ubiquitin ligase membrane-associated ring-CH-type finger 8 (MARCHF8). We have recently demonstrated HPV upregulates MARCHF8 expression keratinocytes head neck (HPV+ HNC) Here, we report stabilizes degrading components SKP1-CUL1-F-box (SCF) complex HPV+ HNC found

Язык: Английский

Siah2- and LRSAM1-mediated K63-linked ubiquitination of snakehead vesiculovirus nucleoprotein facilitates viral replication DOI

Ningyan Jiang,

Hongyan Zhao,

Xiangmou Qin

и другие.

Journal of Virology, Год журнала: 2024, Номер 98(7)

Опубликована: Июнь 6, 2024

ABSTRACT Nucleoprotein (N) is well known for its function in the encapsidation of genomic RNAs negative-strand RNA viruses, which leads to formation ribonucleoproteins that serve as templates viral transcription and replication. However, N protein other aspects during infection far from clear. In this study, snakehead vesiculovirus (SHVV), a kind fish rhabdovirus, was proved be ubiquitinated mainly via K63-linked ubiquitination. We identified nine host E3 ubiquitin ligases interacted with SHVV N, among seven facilitated ubiquitination protein. Further investigation revealed only two ligases, Siah ligase 2 (Siah2) leucine-rich repeat sterile alpha motif containing 1 (LRSAM1), mediated upregulated expression Siah2 LRSAM1, maintained stability N. Besides, overexpression or LRSAM1 promoted replication, while knockdown inhibited Deletion domain did not affect their interactions but reduced summary, mediate facilitate provides novel insights into role proteins viruses. IMPORTANCE Ubiquitination plays an important nucleoprotein viruses has rarely been investigated. This study aimed at investigating rhabdovirus (snakehead vesiculovirus), identifying related determining found ubiquitination, by (Siah 2) (leucine-rich 1). The data suggested were hijacked ubiquitinate exhibited anti-SHVV targets drug design.

Язык: Английский

Процитировано

0

Ubiquitination and degradation of MHC-II by Tim-3 inhibits antiviral immunity DOI
Jie Zhou,

Zhonglin LV,

Meichen Liu

и другие.

Cellular Immunology, Год журнала: 2024, Номер 407, С. 104889 - 104889

Опубликована: Ноя. 7, 2024

Язык: Английский

Процитировано

0

The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer DOI Creative Commons
M. I. Khalil, Canchai Yang, Lexi Vu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 4, 2023

The human papillomavirus (HPV) oncoprotein E7 is a relatively short-lived protein required for HPV-driven cancer development and maintenance. degraded through ubiquitination mediated by cullin 1 (CUL1) the ubiquitin-conjugating enzyme E2 L3 (UBE2L3). However, proteins are maintained at high levels in most HPV-positive cells. A previous proteomics study has shown that UBE2L3 CUL1 increased knockdown of E3 ubiquitin ligase membrane-associated ring-CH-type finger 8 (MARCHF8). We have recently demonstrated HPV upregulates MARCHF8 expression keratinocytes head neck (HPV+ HNC) Here, we report stabilizes degrading components SKP1-CUL1-F-box (SCF) complex HPV+ HNC found

Язык: Английский

Процитировано

0