The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer DOI Creative Commons
M. I. Khalil, Canchai Yang, Lexi Vu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 4, 2023

The human papillomavirus (HPV) oncoprotein E7 is a relatively short-lived protein required for HPV-driven cancer development and maintenance. degraded through ubiquitination mediated by cullin 1 (CUL1) the ubiquitin-conjugating enzyme E2 L3 (UBE2L3). However, proteins are maintained at high levels in most HPV-positive cells. A previous proteomics study has shown that UBE2L3 CUL1 increased knockdown of E3 ubiquitin ligase membrane-associated ring-CH-type finger 8 (MARCHF8). We have recently demonstrated HPV upregulates MARCHF8 expression keratinocytes head neck (HPV+ HNC) Here, we report stabilizes degrading components SKP1-CUL1-F-box (SCF) complex HPV+ HNC found

Язык: Английский

Ubiquitination and degradation of MHC-II by Tim-3 inhibits antiviral immunity DOI
Jie Zhou,

Zhonglin LV,

Meichen Liu

и другие.

Cellular Immunology, Год журнала: 2024, Номер 407, С. 104889 - 104889

Опубликована: Ноя. 7, 2024

Язык: Английский

Процитировано

0
MARCH8 Restricts RSV Replication by Promoting Cellular Apoptosis Through Ubiquitin-Mediated Proteolysis of Viral SH Protein DOI Creative Commons
Takashi Okura,

Tatsuki Takahashi,

T Kameya

и другие.

Viruses, Год журнала: 2024, Номер 16(12), С. 1935 - 1935

Опубликована: Дек. 18, 2024

Numerous host factors function as intrinsic antiviral effectors to attenuate viral replication. MARCH8 is an E3 ubiquitin ligase that has been identified a restriction factor inhibits the replication of various viruses. This study elucidated mechanism by which restricts respiratory syncytial virus (RSV) through selective degradation small hydrophobic (SH) protein. We demonstrated directly interacts with RSV-SH and catalyzes its ubiquitination at lysine 13, leading SH via ubiquitin-lysosomal pathway. Functionally, expression enhances RSV-induced apoptosis degradation, ultimately reducing titers. Conversely, RSV strain harboring SH-K13R mutation exhibited prolonged protein stability attenuated in infected cells, even presence MARCH8. Targeted depletion cellular survival potentially increases persistence. These findings demonstrate promotes early elimination virus-infected cells abrogating anti-apoptotic SH, thereby transmission. Our provides novel insights into interplay between evasion strategies, providing new therapeutic approaches for infections.

Язык: Английский

Процитировано

0
The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer DOI Creative Commons
M. I. Khalil, Canchai Yang, Lexi Vu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 4, 2023

The human papillomavirus (HPV) oncoprotein E7 is a relatively short-lived protein required for HPV-driven cancer development and maintenance. degraded through ubiquitination mediated by cullin 1 (CUL1) the ubiquitin-conjugating enzyme E2 L3 (UBE2L3). However, proteins are maintained at high levels in most HPV-positive cells. A previous proteomics study has shown that UBE2L3 CUL1 increased knockdown of E3 ubiquitin ligase membrane-associated ring-CH-type finger 8 (MARCHF8). We have recently demonstrated HPV upregulates MARCHF8 expression keratinocytes head neck (HPV+ HNC) Here, we report stabilizes degrading components SKP1-CUL1-F-box (SCF) complex HPV+ HNC found

Язык: Английский

Процитировано

0