bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 4, 2023
The
human
papillomavirus
(HPV)
oncoprotein
E7
is
a
relatively
short-lived
protein
required
for
HPV-driven
cancer
development
and
maintenance.
degraded
through
ubiquitination
mediated
by
cullin
1
(CUL1)
the
ubiquitin-conjugating
enzyme
E2
L3
(UBE2L3).
However,
proteins
are
maintained
at
high
levels
in
most
HPV-positive
cells.
A
previous
proteomics
study
has
shown
that
UBE2L3
CUL1
increased
knockdown
of
E3
ubiquitin
ligase
membrane-associated
ring-CH-type
finger
8
(MARCHF8).
We
have
recently
demonstrated
HPV
upregulates
MARCHF8
expression
keratinocytes
head
neck
(HPV+
HNC)
Here,
we
report
stabilizes
degrading
components
SKP1-CUL1-F-box
(SCF)
complex
HPV+
HNC
found
Journal of Virology,
Год журнала:
2024,
Номер
98(7)
Опубликована: Июнь 6, 2024
ABSTRACT
Nucleoprotein
(N)
is
well
known
for
its
function
in
the
encapsidation
of
genomic
RNAs
negative-strand
RNA
viruses,
which
leads
to
formation
ribonucleoproteins
that
serve
as
templates
viral
transcription
and
replication.
However,
N
protein
other
aspects
during
infection
far
from
clear.
In
this
study,
snakehead
vesiculovirus
(SHVV),
a
kind
fish
rhabdovirus,
was
proved
be
ubiquitinated
mainly
via
K63-linked
ubiquitination.
We
identified
nine
host
E3
ubiquitin
ligases
interacted
with
SHVV
N,
among
seven
facilitated
ubiquitination
protein.
Further
investigation
revealed
only
two
ligases,
Siah
ligase
2
(Siah2)
leucine-rich
repeat
sterile
alpha
motif
containing
1
(LRSAM1),
mediated
upregulated
expression
Siah2
LRSAM1,
maintained
stability
N.
Besides,
overexpression
or
LRSAM1
promoted
replication,
while
knockdown
inhibited
Deletion
domain
did
not
affect
their
interactions
but
reduced
summary,
mediate
facilitate
provides
novel
insights
into
role
proteins
viruses.
IMPORTANCE
Ubiquitination
plays
an
important
nucleoprotein
viruses
has
rarely
been
investigated.
This
study
aimed
at
investigating
rhabdovirus
(snakehead
vesiculovirus),
identifying
related
determining
found
ubiquitination,
by
(Siah
2)
(leucine-rich
1).
The
data
suggested
were
hijacked
ubiquitinate
exhibited
anti-SHVV
targets
drug
design.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 4, 2023
The
human
papillomavirus
(HPV)
oncoprotein
E7
is
a
relatively
short-lived
protein
required
for
HPV-driven
cancer
development
and
maintenance.
degraded
through
ubiquitination
mediated
by
cullin
1
(CUL1)
the
ubiquitin-conjugating
enzyme
E2
L3
(UBE2L3).
However,
proteins
are
maintained
at
high
levels
in
most
HPV-positive
cells.
A
previous
proteomics
study
has
shown
that
UBE2L3
CUL1
increased
knockdown
of
E3
ubiquitin
ligase
membrane-associated
ring-CH-type
finger
8
(MARCHF8).
We
have
recently
demonstrated
HPV
upregulates
MARCHF8
expression
keratinocytes
head
neck
(HPV+
HNC)
Here,
we
report
stabilizes
degrading
components
SKP1-CUL1-F-box
(SCF)
complex
HPV+
HNC
found
