bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 4, 2023
The
human
papillomavirus
(HPV)
oncoprotein
E7
is
a
relatively
short-lived
protein
required
for
HPV-driven
cancer
development
and
maintenance.
degraded
through
ubiquitination
mediated
by
cullin
1
(CUL1)
the
ubiquitin-conjugating
enzyme
E2
L3
(UBE2L3).
However,
proteins
are
maintained
at
high
levels
in
most
HPV-positive
cells.
A
previous
proteomics
study
has
shown
that
UBE2L3
CUL1
increased
knockdown
of
E3
ubiquitin
ligase
membrane-associated
ring-CH-type
finger
8
(MARCHF8).
We
have
recently
demonstrated
HPV
upregulates
MARCHF8
expression
keratinocytes
head
neck
(HPV+
HNC)
Here,
we
report
stabilizes
degrading
components
SKP1-CUL1-F-box
(SCF)
complex
HPV+
HNC
found
Viruses,
Год журнала:
2024,
Номер
16(12), С. 1935 - 1935
Опубликована: Дек. 18, 2024
Numerous
host
factors
function
as
intrinsic
antiviral
effectors
to
attenuate
viral
replication.
MARCH8
is
an
E3
ubiquitin
ligase
that
has
been
identified
a
restriction
factor
inhibits
the
replication
of
various
viruses.
This
study
elucidated
mechanism
by
which
restricts
respiratory
syncytial
virus
(RSV)
through
selective
degradation
small
hydrophobic
(SH)
protein.
We
demonstrated
directly
interacts
with
RSV-SH
and
catalyzes
its
ubiquitination
at
lysine
13,
leading
SH
via
ubiquitin-lysosomal
pathway.
Functionally,
expression
enhances
RSV-induced
apoptosis
degradation,
ultimately
reducing
titers.
Conversely,
RSV
strain
harboring
SH-K13R
mutation
exhibited
prolonged
protein
stability
attenuated
in
infected
cells,
even
presence
MARCH8.
Targeted
depletion
cellular
survival
potentially
increases
persistence.
These
findings
demonstrate
promotes
early
elimination
virus-infected
cells
abrogating
anti-apoptotic
SH,
thereby
transmission.
Our
provides
novel
insights
into
interplay
between
evasion
strategies,
providing
new
therapeutic
approaches
for
infections.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 4, 2023
The
human
papillomavirus
(HPV)
oncoprotein
E7
is
a
relatively
short-lived
protein
required
for
HPV-driven
cancer
development
and
maintenance.
degraded
through
ubiquitination
mediated
by
cullin
1
(CUL1)
the
ubiquitin-conjugating
enzyme
E2
L3
(UBE2L3).
However,
proteins
are
maintained
at
high
levels
in
most
HPV-positive
cells.
A
previous
proteomics
study
has
shown
that
UBE2L3
CUL1
increased
knockdown
of
E3
ubiquitin
ligase
membrane-associated
ring-CH-type
finger
8
(MARCHF8).
We
have
recently
demonstrated
HPV
upregulates
MARCHF8
expression
keratinocytes
head
neck
(HPV+
HNC)
Here,
we
report
stabilizes
degrading
components
SKP1-CUL1-F-box
(SCF)
complex
HPV+
HNC
found