Comparative genomic analysis uncovered phylogenetic diversity, evolution of virulence factors, and horizontal gene transfer events in tomato bacterial spot Xanthomonas euvesicatoria DOI Creative Commons
Chien‐Jui Huang, Ting-Li Wu,

Yu-Lin Wu

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 5, 2024

Introduction Bacterial spot, caused by diverse xanthomonads classified into four lineages within three species, poses a significant threat to global pepper and tomato production. In Taiwan, bacterial spot phylogenetically related an atypical Xanthomonas euvesicatoria pv. perforans ( Xep ) strain NI1 from Nigeria were found. Methods To investigate the genetic structure of Taiwanese strains determine phylogenetic position strains, we completed high-quality, gap-free, circularized genomes seven performed comparative genomic analyses with X. pathovars. Average nucleotide identity, core genome analysis, phylogenomic analysis conducted. Results Three sequenced identified as typical , while clustered NI1, forming distinct genomovar euvesicatoria, proposed taiwanensis Xet ). This new lineage likely originated in Taiwan spread through seed trade. At level, chromosomes remained conserved among plasmids contributed virulence, avirulence, field fitness. Gap-free revealed associations between evolution type III effectors, horizontal gene transfer events, plasmid diversity, recombination. Discussion study highlights critical roles shaping makeup, evolution, environmental adaptation plant pathogenic xanthomonads. The identification genomovar, provides insights diversity pathogens

Язык: Английский

Evolution and spread of Xanthomonas citri subsp. citri in the São Paulo, Brazil, citrus belt inferred from 758 novel genomes DOI Creative Commons
Caio Felipe Cavicchia Zamunér,

Dennis Carhuaricra-Huaman,

Roobinidevi Ragupathy

и другие.

Microbial Genomics, Год журнала: 2025, Номер 11(1)

Опубликована: Янв. 16, 2025

The São Paulo state citrus belt in Brazil is a major production region. Since at least 1957, plantations this region have been affected by canker, an economically damaging disease caused Xanthomonas citri subsp. (Xcc). For about 50 years, until 2017, canker eradication programme was carried out In work, our aim to investigate the effects of on genetic variability and evolution Xcc. To end, we sequenced analysed 758 Xcc genomes sampled belt, together with 730 publicly available from around world. Our phylogenomic analyses show that these can be grouped into seven lineages Paulo, lineage L7 dominant. time estimate for its appearance closely matches date when expanded. subdivided L7.1 L7.2. samples, L7.2, which emerged 1964, far most abundant, showing had little impact strain diversification. On other hand, oscillations estimated effective population size L7.2 strains over match shifts programme. sum, present detailed view genomic diversity world largest such effort terms number crop pathogen undertaken so far. methods employed here form basis active surveillance areas.

Язык: Английский

Процитировано

0

Diversification of an emerging bacterial plant pathogen; insights into the global spread of Xanthomonas euvesicatoria pv. perforans DOI Creative Commons
Sujan Timilsina,

Fernanda Iruegas-Bocardo,

Mustafa O. Jibrin

и другие.

PLoS Pathogens, Год журнала: 2025, Номер 21(4), С. e1013036 - e1013036

Опубликована: Апрель 9, 2025

Emerging and re-emerging plant diseases continue to present multifarious threats global food security. Considerable recent efforts are therefore being channeled towards understanding the nature of pathogen emergence, their spread evolution. Xanthomonas euvesicatoria pv. perforans (Xep ) , one causal agents bacterial spot tomato, rapidly emerged displaced other xanthomonads in many tomato production regions around world. In less than three decades, it has become a dominant xanthomonad systems across world presents compelling example for diversification recently pathogens. Although Xep been continuously monitored Florida since its discovery, population structure evolution at genome-scale is yet be fully explored. The objectives this work were determine genetic diversity globally ascertain if different contain genetically distinct populations, examine relatedness strains collected seed areas East Asia regions, evaluate variation type III secretion effectors, which critical pathogenicity virulence factors, relationship structure. We used genome data from 270 13 countries phylogenetic analysis characterization effector gene among strains. Our results showed notable pathogen. found similar distant including over past 100 years, consistent with intercontinental dissemination hybrid chains. Evolution pangenome, acquisition loss secreted apparent within lineages. long-distance movement pathogen, together variants that may not widely distributed, poses risks emergence new production.

Язык: Английский

Процитировано

0

Xanthomonas as a model system for studying pathogen emergence and evolution DOI
Sujan Timilsina,

Amandeep Kaur,

Anuj Sharma

и другие.

Phytopathology, Год журнала: 2024, Номер 114(7), С. 1433 - 1446

Опубликована: Апрель 22, 2024

In this review, we highlight studies in which whole-genome sequencing, comparative genomics, and population genomics have provided unprecedented insights into past ongoing pathogen evolution. These include new understandings of the adaptive evolution secretion systems their effectors. We focus on

Язык: Английский

Процитировано

2

Intercontinental dissemination and diversification ofXanthomonas perforansin tomato production DOI Creative Commons
Sujan Timilsina,

Fernanda Iruegas-Bocardo,

Mustafa O. Jibrin

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 22, 2024

Abstract Emerging and re-emerging plant diseases continue to present multifarious threats global food security. Considerable recent efforts are therefore being channeled towards understanding the nature of pathogen emergence, their spread evolution. Xanthomonas euvesicatoria pv. perforans (Xep ), one causal agents bacterial spot tomato, rapidly emerged displaced other xanthomonads in tomato production regions around world. In less than three decades, it has become a dominant xanthomonad systems across world presents model for diversification recently pathogens. Although Xep been continuously monitored Florida since its discovery, population structure evolution at genome-scale is yet be fully explored. The objectives this work were determine genetic diversity globally ascertain if different contain genetically distinct populations, examine relatedness strains collected seed areas East Asia regions, evaluate variation type III effectors, which critical pathogenicity virulence factors, relationship structure. We used genome data from 270 13 countries phylogenetic analysis characterization Xop effector gene among strains. Our results showed notable pathogen. found similar distant including over past 100 years, consistent with intercontinental dissemination hybrid chains. Evolution pangenome, acquisition loss secreted apparent within lineages. long-distance movement pathogen, together variants that may not widely distributed, poses risks emergence new production.

Язык: Английский

Процитировано

1

Comparative genomic analysis uncovered phylogenetic diversity, evolution of virulence factors, and horizontal gene transfer events in tomato bacterial spot Xanthomonas euvesicatoria DOI Creative Commons
Chien‐Jui Huang, Ting-Li Wu,

Yu-Lin Wu

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 5, 2024

Introduction Bacterial spot, caused by diverse xanthomonads classified into four lineages within three species, poses a significant threat to global pepper and tomato production. In Taiwan, bacterial spot phylogenetically related an atypical Xanthomonas euvesicatoria pv. perforans ( Xep ) strain NI1 from Nigeria were found. Methods To investigate the genetic structure of Taiwanese strains determine phylogenetic position strains, we completed high-quality, gap-free, circularized genomes seven performed comparative genomic analyses with X. pathovars. Average nucleotide identity, core genome analysis, phylogenomic analysis conducted. Results Three sequenced identified as typical , while clustered NI1, forming distinct genomovar euvesicatoria, proposed taiwanensis Xet ). This new lineage likely originated in Taiwan spread through seed trade. At level, chromosomes remained conserved among plasmids contributed virulence, avirulence, field fitness. Gap-free revealed associations between evolution type III effectors, horizontal gene transfer events, plasmid diversity, recombination. Discussion study highlights critical roles shaping makeup, evolution, environmental adaptation plant pathogenic xanthomonads. The identification genomovar, provides insights diversity pathogens

Язык: Английский

Процитировано

1