Proviral role of ATG2 autophagy related protein in tomato bushy stunt virus replication through bulk phospholipid transfer into the viral replication organelle
Molecular Biology of the Cell,
Год журнала:
2024,
Номер
35(10)
Опубликована: Авг. 7, 2024
Subversion
of
cellular
membranes
and
membrane
proliferation
are
used
by
positive-strand
RNA
viruses
to
build
viral
replication
organelles
(VROs)
that
support
virus
replication.
The
biogenesis
the
membranous
VROs
requires
major
changes
in
lipid
metabolism
transfer
infected
cells.
In
this
work,
we
show
tomato
bushy
stunt
(TBSV)
hijacks
Atg2
autophagy
related
protein
with
bulk
activity
into
via
interaction
TBSV
p33
protein.
Deletion
yeast
knockdown
Язык: Английский
Autophagy machinery as exploited by viruses
Autophagy Reports,
Год журнала:
2025,
Номер
4(1)
Опубликована: Март 18, 2025
Viruses
adapt
and
modulate
cellular
pathways
to
allow
their
replication
in
host
cells.
The
catabolic
pathway
of
macroautophagy,
for
simplicity
referred
as
autophagy,
is
no
exception.
In
this
review,
we
discuss
anti-viral
functions
both
autophagy
select
components
the
machinery,
how
viruses
have
evaded
them.
Some
use
membrane
remodeling
ability
machinery
build
compartments
cytosol
or
efficiently
egress
from
cells
a
non-lytic
fashion.
remodeled
membranes
can
even
be
found
viral
particles
envelopes
single
around
virus
packages
that
protect
them
during
spreading
transmission.
Therefore,
studies
on
regulation
by
infections
reveal
beyond
lysosomal
degradation
cytosolic
constituents.
Furthermore,
they
also
pinpoint
molecular
interactions
with
which
most
manipulated,
may
relevant
develop
effective
disease
treatments
based
modulation.
Язык: Английский
Screening bacterial effectors and human virus proteins in yeast to identify host factors driving tombusvirus RNA recombination: a role for autophagy and membrane phospholipid content
Journal of Virology,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 27, 2025
ABSTRACT
Recombination
in
RNA
viruses
contributes
to
virus
evolution
and
rapid
emergence
of
new
viral
variants
that
helps
evade
host’s
antiviral
strategies.
Host
factors
play
important
but
poorly
characterized
roles
recombination.
The
authors
expressed
Legionella
bacterium
effector
proteins
SARS-CoV-2
human
metapneumovirus
(HMPV)
yeast
test
their
effects
on
tomato
bushy
stunt
(TBSV)
identified
16
effectors,
six
SARS-CoV-2,
two
HMPV
affecting
TBSV
recombination
likely
target
shared
host
with
TBSV.
Among
the
targets
effectors/viral
was
autophagy
pathway.
Inhibition
by
expression
RavZ
LegA9
effectors
reduced
production
recombinants
plants.
Induction
rapamycin,
via
nitrogen
starvation
or
overexpression
ATG2
lipid
transfer
protein,
led
enhanced
Using
vitro
replicase
assembly
giant
unilamellar
vesicles
confirmed
critical
role
phosphatidylethanolamine
We
suggest
pro-recombination
co-opted
is
provide
abundant
phospholipids
for
replication
organelle
biogenesis.
Overall,
this
work
highlights
membrane
context
regulation
show
N
M2-1
enhance
protecting
RNAs
from
Xrn1
5´−3´
exoribonuclease
yeast.
Altogether,
novel
strategy
using
as
a
cellular
system
sensor
might
assist
identification
functional
various
bacterial
IMPORTANCE
Positive-strand
(+)RNA
replicate
cytosol
infected
cells
exploiting
resources
frequently
lead
diseases.
Virus
results
generation
contribute
adaptation
hosts.
proteins,
This
approach
revealed
heterologous
TBSV,
including
In
replication.
nucleocapsid
protein
are
shown
Thus,
TBSV/yeast
can
be
used
find
functions
proteins.
Язык: Английский
Novel exploitation of autophagy by tombusviruses
Virology,
Год журнала:
2024,
Номер
603, С. 110363 - 110363
Опубликована: Дек. 18, 2024
Язык: Английский
Mobilization of nuclear antiviral factors by Exportin XPO1 via the actin network inhibits RNA virus replication
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 20, 2024
ABSTRACT
The
intricate
interplay
between
+RNA
viruses
and
their
hosts
involves
the
exploitation
of
host
resources
to
build
virus-induced
membranous
replication
organelles
(VROs)
in
cytosol
infected
cells.
Previous
genome-
proteome-wide
approaches
have
identified
numerous
nuclear
proteins,
including
restriction
factors
that
affect
tomato
bushy
stunt
virus
(TBSV).
However,
it
is
currently
unknown
how
cells
mobilize
antiviral
proteins
tombusviruses
manipulate
nuclear-cytoplasmic
communication.
authors
discovered
XPO1/CRM1
exportin
plays
a
central
role
TBSV
plants.
Based
on
knockdown,
chemical
inhibition,
transient
expression
vitro
experiments,
we
show
XPO1
acts
as
cellular
factor
against
TBSV.
recruited
by
p33
protein
into
cytosolic
VROs
via
direct
interaction.
Blocking
nucleocytoplasmic
transport
function
inhibits
delivery
several
resulting
dampened
effects.
co-opted
actin
network
critical
for
deliver
activities.
We
XPO1-delivered
accumulate
vir-condensates
associated
with
VROs.
Altogether,
emerging
theme
complex:
propose
vir-condensate
serves
battleground
supremacy
controlling
infection.
It
seems
balance
pro-viral
within
could
be
major
determining
susceptibility.
conclude
cargos
are
key
players
communication
during
replication.
Significance
Tomato
(TBSV),
similar
other
(+)RNA
viruses,
replicates
exploits
organellar
membrane
surfaces
viral
represent
sites
shuttle
inhibited
conserved
interaction
nod,
which
propelled
restricted
delivered
provided
inhibitory
functions
condensates
VRO-associated
condensate
hub
implications
its
Язык: Английский