Molecular Evidence of Raccoon Dog (Nyctereutes procyonoides) as a Natural Definitive Host for Several Sarcocystis Species
Pathogens,
Год журнала:
2025,
Номер
14(3), С. 288 - 288
Опубликована: Март 15, 2025
Sarcocystis
parasites
infect
a
wide
range
of
animals,
including
reptiles,
birds,
and
mammals,
have
complex
two-host
prey–predator
life
cycle.
Sarcocysts
are
mainly
found
in
the
muscles
intermediate
hosts,
oocysts
sporulate
intestines
definitive
host.
The
raccoon
dog
(Nyctereutes
procyonoides),
native
to
Asia
invasive
Europe,
is
known
disease
carrier.
However,
studies
on
dogs
transmission
scarce.
Between
2019
2024,
total
26
carcasses
were
collected
Lithuania.
results
light
microscopy
examination
indicated
that
50%
samples
positive
for
spp.
sporocysts
sporulated
oocysts.
Based
nested
PCR
sequencing
cox1,
88.5%
these
parasites.
Molecular
analysis
revealed
presence
11
different
species.
Eight
species,
S.
alces,
capracanis,
hjorti,
iberica,
linearis,
morae,
tenella,
venatoria
reported
first
time
as
hosts.
identified
species
linked
such
cervids,
wild
boars,
pigs,
goats,
sheep.
These
findings
suggest
play
key
role
spread
Sarcocystis,
particularly
infecting
cervids.
Язык: Английский
SARS-CoV-2 spike protein: structure, viral entry and variants
Nature Reviews Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 6, 2025
Язык: Английский
Discovery of Nanosota-9 as anti-Omicron nanobody therapeutic candidate
PLoS Pathogens,
Год журнала:
2024,
Номер
20(11), С. e1012726 - e1012726
Опубликована: Ноя. 26, 2024
Omicron
subvariants
of
SARS-CoV-2
continue
to
pose
a
significant
global
health
threat.
Nanobodies,
single-domain
antibodies
derived
from
camelids,
are
promising
therapeutic
tools
against
pandemic
viruses
due
their
favorable
properties.
In
this
study,
we
identified
novel
nanobody,
Nanosota-9,
which
demonstrates
high
potency
wide
range
both
in
vitro
and
mouse
model.
Cryo-EM
data
revealed
that
Nanosota-9
neutralizes
through
unique
mechanism:
two
molecules
crosslink
receptor-binding
domains
(RBDs)
the
trimeric
spike
protein,
preventing
RBDs
binding
ACE2
receptor.
This
mechanism
explains
its
strong
anti-Omicron
potency.
Additionally,
epitopes
on
protein
relatively
conserved
among
subvariants,
contributing
broad
spectrum.
Combined
with
our
recently
developed
structure-guided
evolution
approach
for
nanobodies,
has
potential
serve
as
foundation
superior
therapeutic.
Язык: Английский
Receptor binding and structural basis of raccoon dog ACE2 binding to SARS-CoV-2 prototype and its variants
PLoS Pathogens,
Год журнала:
2024,
Номер
20(12), С. e1012713 - e1012713
Опубликована: Дек. 5, 2024
Raccoon
dog
was
proposed
as
a
potential
host
of
SARS-CoV-2,
but
no
evidence
support
such
notion.
In
our
study,
we
investigated
the
binding
affinities
raccoon
ACE2
(rdACE2)
to
spike
(S)
protein
receptor
domain
(RBD)
SARS-CoV-2
prototype
(PT)
and
its
variants.
It
revealed
that
RBD
from
variants
were
generally
lower
than
PT
RBD.
Through
structural
functional
analyses,
found
amino
acids
H34
M82
play
pivotal
roles
in
maintaining
affinity
different
sub-variants.
These
results
suggest
dogs
exhibit
susceptibility
compared
those
animal
species
with
high
prevalence
transmission.
Язык: Английский