Tissue‐resident memory T cells and lung immunopathology
Immunological Reviews,
Год журнала:
2023,
Номер
316(1), С. 63 - 83
Опубликована: Апрель 4, 2023
Rapid
reaction
to
microbes
invading
mucosal
tissues
is
key
protect
the
host
against
disease.
Respiratory
tissue-resident
memory
T
(T
Язык: Английский
Bacterial-induced or passively administered interferon gamma conditions the lung for early control of SARS-CoV-2
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Дек. 12, 2023
Type-1
and
type-3
interferons
(IFNs)
are
important
for
control
of
viral
replication;
however,
less
is
known
about
the
role
Type-2
IFN
(IFNγ)
in
anti-viral
immunity.
We
previously
observed
that
lung
infection
with
Mycobacterium
bovis
BCG
achieved
though
intravenous
(iv)
administration
provides
strong
protection
against
SARS-CoV-2
mice
yet
drives
low
levels
type-1
IFNs
but
robust
IFNγ.
Here
we
examine
ongoing
IFNγ
responses
to
pre-established
bacterial
on
disease
outcomes
two
murine
models.
report
required
iv
induced
reduction
pulmonary
loads,
an
outcome
dependent
receptor
expression
by
non-hematopoietic
cells.
Importantly,
show
prompts
epithelial
cells
upregulate
IFN-stimulated
genes
reported
activity
IFNγ-dependent
manner,
suggesting
a
possible
mechanism
protection.
Finally,
confirm
properties
demonstrating
recombinant
cytokine
itself
challenge
when
administered
intranasally.
Together,
our
data
response
within
protective
infection,
concurrent
or
recent
infections
drive
may
limit
pathogenesis
supporting
prophylactic
uses
COVID-19
management.
Язык: Английский
Th2 and Th17‐associated immunopathology following SARS‐CoV‐2 breakthrough infection in Spike‐vaccinated ACE2‐humanized mice
Journal of Medical Virology,
Год журнала:
2024,
Номер
96(1)
Опубликована: Янв. 1, 2024
Abstract
Vaccines
have
demonstrated
remarkable
effectiveness
in
protecting
against
COVID‐19;
however,
concerns
regarding
vaccine‐associated
enhanced
respiratory
diseases
(VAERD)
following
breakthrough
infections
emerged.
Spike
protein
subunit
vaccines
for
SARS‐CoV‐2
induce
VAERD
hamsters,
where
aluminum
adjuvants
promote
a
Th2‐biased
immune
response,
leading
to
increased
type
2
pulmonary
inflammation
animals
with
infections.
To
gain
deeper
understanding
of
the
potential
risks
and
underlying
mechanisms
VAERD,
we
immunized
ACE2‐humanized
mice
adjuvanted
CpG‐ODN.
Subsequently,
exposed
them
increasing
doses
establish
infection.
The
vaccine
elicited
robust
neutralizing
antibody
responses,
reduced
viral
titers,
host
survival.
However,
infection,
vaccinated
exhibited
severe
immunopathology,
characterized
by
significant
perivascular
infiltration
eosinophils
CD4
+
T
cells,
along
expression
Th2/Th17
cytokines.
Intracellular
flow
cytometric
analysis
revealed
systemic
Th17
inflammatory
particularly
pronounced
lungs.
Our
data
demonstrate
that
aluminum/CpG
strong
Th1‐associated
immunity
COVID‐19
but
also
prime
which
may
contribute
rapid
onset
cell‐mediated
immunopathology
These
findings
underscore
necessity
further
research
unravel
complexities
enhance
formulations
broad
protection
maximum
safety.
Язык: Английский
Anti-Viral Activity of Bioactive Molecules of Silymarin against COVID-19 via In Silico Studies
Pharmaceuticals,
Год журнала:
2023,
Номер
16(10), С. 1479 - 1479
Опубликована: Окт. 17, 2023
The
severe
acute
respiratory
syndrome-coronavirus-2
(SARS-CoV-2)
infection
drove
the
global
coronavirus
disease
2019
(COVID-19)
pandemic,
causing
a
huge
loss
of
human
life
and
negative
impact
on
economic
development.
It
is
an
urgent
necessity
to
explore
potential
drugs
against
viruses,
such
as
SARS-CoV-2.
Silymarin,
mixture
herb-derived
polyphenolic
flavonoids
extracted
from
milk
thistle,
possesses
potent
antioxidative,
anti-apoptotic,
anti-inflammatory
properties.
Accumulating
research
studies
have
demonstrated
killing
activity
silymarin
dengue
virus,
chikungunya
hepatitis
C
virus.
However,
anti-COVID-19
mechanisms
remain
unclear.
In
this
study,
multiple
disciplinary
approaches
methodologies
were
applied
evaluate
anti-viral
agent
SARS-CoV-2
infection.
silico
molecular
docking,
network
pharmacology,
bioinformatic
methods
incorporated
assess
ligand–protein
binding
properties
analyze
protein–protein
interaction
network.
DAVID
database
was
used
gene
functions,
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
pathway
Gene
Ontology
(GO)
enrichment.
TCMSP
GeneCards
identify
drug
target
genes
COVID-19-related
genes.
Our
results
revealed
that
compounds,
silybin
A/B
silymonin,
displayed
triplicate
functions
infection,
including
directly
with
angiotensin-converting
enzyme
2
(ACE2)
inhibit
entry
into
host
cells,
viral
proteins
RdRp
helicase
replication
proliferation,
regulating
immune
response
indirectly
Specifically,
targets
molecules
in
regulation
screened
out,
proinflammatory
cytokines
TNF
IL-6
cell
growth
factors
VEGFA
EGF.
addition,
mechanism
drug-target
protein
investigated,
pockets
ACE2
proteins,
formation
hydrogen
bonds,
hydrophobic
interactions,
other
drug–protein
ligand
interactions.
Finally,
drug-likeness
candidate
passed
criteria
for
screening.
Overall,
study
demonstrates
Язык: Английский
Interaction dynamics between innate and adaptive immune cells responding to SARS-CoV-2 vaccination in non-human primates
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Дек. 2, 2023
Abstract
As
SARS-CoV-2
variants
continue
evolving,
testing
updated
vaccines
in
non-human
primates
remains
important
for
guiding
human
clinical
practice.
To
date,
such
studies
have
focused
on
antibody
titers
and
antigen-specific
B
T
cell
frequencies.
Here,
we
extend
our
understanding
by
integrating
innate
adaptive
immune
responses
to
mRNA-1273
vaccination
rhesus
macaques.
We
sorted
cells
from
a
pre-vaccine
time
point,
as
well
peripheral
two
weeks
after
each
of
vaccine
doses
used
single-cell
sequencing
assess
the
transcriptomes
receptors
cell.
show
that
subset
S-specific
expresses
cytokines
critical
activating
responses,
with
concomitant
increase
CCR5-expressing
intermediate
monocytes
shift
natural
killer
more
cytotoxic
phenotype.
The
second
dose,
administered
4
first,
elicits
an
circulating
germinal
center-like
2
later,
which
are
clonally
expanded
enriched
epitopes
receptor
binding
domain.
Both
stimulate
inflammatory
response
genes
associated
elevated
production.
Overall,
provide
comprehensive
picture
bidirectional
signaling
between
components
system
suggest
potential
mechanisms
enhanced
secondary
exposure.
Язык: Английский
Pivotal role of tissue-resident memory lymphocytes in the control of mucosal infections: can mucosal vaccination induce protective tissue-resident memory T and B cells?
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Сен. 11, 2023
OPINION
article
Front.
Immunol.,
11
September
2023Sec.
Immunological
Memory
Volume
14
-
2023
|
https://doi.org/10.3389/fimmu.2023.1216402
Язык: Английский