Phage Therapy: A Targeted Approach to Overcoming Antibiotic Resistance
Microbial Pathogenesis,
Год журнала:
2024,
Номер
unknown, С. 107088 - 107088
Опубликована: Окт. 1, 2024
Язык: Английский
A flagella-dependent Burkholderia jumbo phage controls rice seedling rot and steers Burkholderia glumae toward reduced virulence in rice seedlings
mBio,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 27, 2025
ABSTRACT
Bacteriophages
(phages)
are
being
investigated
as
potential
biocontrol
agents
for
the
suppression
of
bacterial
diseases
in
cultivated
crops.
Jumbo
bacteriophages,
which
possess
genomic
DNA
larger
than
200
kbp,
generally
have
a
broader
host
range
other
phages
and
therefore
would
be
useful
against
wide
strains.
Thus,
characterization
novel
jumbo
specific
agricultural
pathogens
importance
development
phage
strategies.
Herein,
we
demonstrate
that
S13
requires
Burkholderia
glumae
flagella
its
attachment
infection
loss
B.
prevents
cellular
lysis.
As
is
known
virulence
factor,
results
surviving
population
with
reduced
virulence.
Further
experimentation
demonstrates
can
protect
rice
plants
from
-sponsored
destruction
seedling
model
infection.
IMPORTANCE
Bacterial
plant
threaten
many
major
food
crops
inflict
large
losses
worldwide.
pathogen
causes
such
rot,
wilt,
blight
several
including
rice,
tomato,
hot
pepper,
eggplant.
infects
during
all
developmental
stages,
causing
rot
panicle
(BPB).
The
incidence
predicted
to
increase
warming
global
temperatures,
different
control
strategies
targeting
explored.
These
include
chemical
antibiotic
soil
amendment,
microbiome
manipulation,
use
partially
resistant
cultivars.
However,
despite
growth
amelioration,
treatment
options
infections
remain
limited
cultural
practices.
Alternatively,
represents
promising
new
method
eliminating
crop
soils
improving
yields.
Язык: Английский
Enhanced bacteriostatic effects of phage vB_C4 and cell wall-targeting antibiotic combinations against drug-resistant Aeromonas veronii
Microbiology Spectrum,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 16, 2025
Aeromonas
veronii
is
a
vital
zoonotic
pathogen
known
for
its
extensive
drug
resistance
and
ability
to
form
biofilms,
which
contribute
antibiotic
resistance.
In
this
study,
the
phage
vB_C4,
specifically
targeting
A.
veronii,
was
isolated
subjected
bioinformatic
analysis
bacteriostatic
activity
assays.
The
combination
of
vB_C4
with
antibiotics
such
as
cephalothin
cefoxitin,
target
bacterial
cell
wall,
resulted
in
significantly
enhanced
effect
compared
either
or
alone.
Furthermore,
dosage
critical
optimizing
antimicrobial
when
used
conjunction
antibiotics.
This
combined
treatment
exhibited
more
distinct
removing
mature
biofilms
inhibiting
biofilm
formation,
leading
considerable
decrease
density
within
biofilm.
Overall,
synergistic
use
offers
novel
attitude
treating
pathogenic
bacteria
holds
significant
potential
preventing
emergence
drug-resistant
strains.IMPORTANCEThe
application
phages
not
only
effectively
inhibits
phage-resistant
but
also
reduces
required
effective
concentration
Additionally,
therapy
demonstrates
therapeutic
effects
on
clinical
infections
mediated
by
biofilms.
Consequently,
study
establishes
basis
evaluating
parameters
essential
utilizing
phage-antibiotic
biofilm-associated
infections,
thereby
offering
selection
management
multidrug-resistant
infections.
Язык: Английский
Bacterial Extracellular Vesicles and Antimicrobial Peptides: A Synergistic Approach to Overcome Antimicrobial Resistance
Antibiotics,
Год журнала:
2025,
Номер
14(4), С. 414 - 414
Опубликована: Апрель 19, 2025
Antimicrobial
resistance
is
already
a
major
global
health
threat,
contributing
to
nearly
5
million
deaths
annually.
The
rise
of
multidrug-resistant
pathogens
has
made
many
infections
increasingly
difficult
treat.
This
growing
threat
driven
the
search
for
alternative
therapeutic
approaches.
Among
most
promising
candidates
are
bacterial
extracellular
vesicles
(BEVs)
and
antimicrobial
peptides
(AMPs),
which
offer
unique
mechanisms
action,
potential
synergistic
effects,
ability
bypass
conventional
pathways.
review
summarizes
current
research
on
effects
BEVs
AMPs
overcome
resistance.
Язык: Английский
Antimicrobial efflux and biofilms: an interplay leading to emergent resistance evolution
Trends in Microbiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 1, 2025
The
biofilm
mode
of
growth
and
drug
efflux
are
both
important
factors
that
impede
the
treatment
bacterial
infections
with
antimicrobials.
Decades
work
have
uncovered
mechanisms
involved
in
biofilm-mediated
antimicrobial
tolerance,
but
links
between
these
phenomena
only
recently
been
discovered.
Novel
findings
show
how
impacts
global
cellular
physiology
antibiotic
underpinned
by
phenotypic
heterogeneity.
In
addition
can
mediate
cell-to-cell
interactions,
relevant
biofilms,
via
including
signaling
molecules
metabolites,
using
pump
components
establishment
local
gradients
pumping.
These
recent
suggest
tolerance
closely
coupled,
synergistic
effects
leading
to
evolution
resistance
environment.
Язык: Английский
Heterogeneous efflux pump expression underpins phenotypic resistance to antimicrobial peptides
Опубликована: Май 23, 2025
Antimicrobial
resistance
threatens
the
viability
of
modern
medical
interventions.
There
is
a
dire
need
developing
novel
approaches
to
counter
mechanisms
employed
by
starved
or
slow-growing
pathogens
that
are
refractory
conventional
antimicrobial
therapies.
peptides
have
been
advocated
as
potential
therapeutic
solutions
due
low
levels
genetic
observed
in
bacteria
against
these
compounds.
However,
here
we
show
subpopulations
stationary
phase
Escherichia
coli
and
Pseudomonas
aeruginosa
survive
tachyplesin
treatment
without
mutations.
These
phenotypic
variants
display
enhanced
efflux
activity
limit
intracellular
peptide
accumulation.
Differential
regulation
genes
involved
outer
membrane
vesicle
section,
modification,
protease
were
also
found
between
phenotypically
resistant
susceptible
cells.
We
discovered
formation
could
be
prevented
administering
combination
with
sertraline,
clinically
used
antidepressant,
suggesting
approach
for
combatting
antimicrobial-refractory
bacteria.
Язык: Английский
Antibiotic resistant bacteria survive treatment by doubling while shrinking
mBio,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 20, 2024
Many
antibiotics
that
are
used
in
healthcare,
farming,
and
aquaculture
end
up
environments
with
different
spatial
structures
might
promote
heterogeneity
the
emergence
of
antibiotic
resistance.
However,
experimental
evolution
microbes
at
sub-inhibitory
concentrations
has
been
mainly
carried
out
population
level
which
does
not
allow
capturing
single-cell
responses
to
antibiotics.
Here,
we
investigate
compare
resistance
ciprofloxacin
Язык: Английский