Extensive longevity and DNA virus-driven adaptation in nearctic Myotis bats DOI Creative Commons
Juan Manuel Vázquez, M. Elise Lauterbur, Saba Mottaghinia

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 11, 2024

Abstract The genus Myotis is one of the largest clades bats, and exhibits some most extreme variation in lifespans among mammals alongside unique adaptations to viral tolerance immune defense. To study evolution longevity-associated traits infectious disease, we generated near-complete genome assemblies cell lines for 8 closely related species . Using genome-wide screens positive selection, analyses structural variation, functional experiments primary lines, identify new patterns adaptation contributing longevity, cancer resistance, interactions bats. We find that bats have significant risk across demonstrate a DNA damage response cells long-lived M. lucifugus also evidence abundant viruses - but not RNA other sharp contrast with mammals, potentially role as reservoirs zoonoses. Together, our results how genomics derived from diverse taxa uncover molecular bases non-model organisms.

Язык: Английский

Processing of Genomic RNAs by Dicer in Bat Cells Limits SARS-CoV-2 Replication DOI Open Access
Iyanuoluwani Owolabi,

Shazeed-UI Karim,

Sweta Khanal

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 10, 2025

ABSTRACT Bats are reservoirs for numerous viruses that cause serious diseases in other animals and humans. Several mechanisms proposed to contribute the tolerance of bats these pathogens. This study investigates response bat cells double-stranded RNA generated by SARS-CoV-2 replication. Here, we found involvement Dicer processing viral genomic RNAs during infection. Examining sequencing infected cells, small-interfering (siRNA)-like fragments were derived from RNAs. Depletion showed a reduction an increase loads suggesting unlike mammals, may use limit prompted exploration key dsRNA sensors cells. Our analysis significant upregulation OAS1 MX1 dsRNA, while PKR levels remained low, alternative dsRNA-response present eschew common PKR-based system. These results further show how employ distinct strategies antiviral defense tolerating infections. They suggest bats, function not observed mammals. highlights mechanism originating evolve features could extreme responses such as is seen with SARS-CoV-2. GRAPHICAL SUMMARY

Язык: Английский

Процитировано

0
Bat adaptations in inflammation and cell death regulation contribute to viral tolerance DOI Creative Commons
Subham Das, Disha Jain, Priyanka Chaudhary

и другие.

mBio, Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

ABSTRACT Bats are reservoirs for multiple viruses, some of which known to cause global disease outbreaks. Virus spillovers from bats have been implicated in zoonotic transmission. Some bat species can tolerate viral infections, such as infections with coronaviruses and paramyxoviruses, better than humans less clinical consequences. Bat speculated evolved alongside these pathogens, adaptations within the immune system considered be associated tolerance. Inflammation cell death response virus prime human immunopathology. Unlike humans, mitigate infection-induced inflammation. Inflammatory pathways necroptosis pyroptosis immunopathology during but their regulation remains understudied. This review focuses on inflammation bats. We also provide a perspective possible contribution death-regulating proteins, caspases gasdermins, modulating tissue damage Understanding role responses valuable insights managing future outbreaks, addressing severity, improving pandemic preparedness.

Язык: Английский

Процитировано

0
Processing of genomic RNAs by Dicer in bat cells limits SARS-CoV-2 replication DOI Creative Commons
Iyanuoluwani Owolabi,

Shazeed-Ul Karim,

Sweta Khanal

и другие.

Virology Journal, Год журнала: 2025, Номер 22(1)

Опубликована: Март 25, 2025

Bats are reservoirs for numerous viruses that cause serious diseases in other animals and humans. Several mechanisms proposed to contribute the tolerance of bats these pathogens. This study investigates response bat cells double-stranded RNA generated by SARS-CoV-2 replication. Here, we found involvement Dicer processing viral genomic RNAs during infection. Examining sequencing infected cells, small-interfering (siRNA)-like fragments were derived from RNAs. Depletion showed a reduction an increase loads suggesting unlike mammals, may use limit prompted exploration key dsRNA sensors cells. Our analysis significant upregulation OAS1 MX1 dsRNA, while PKR levels remained low, alternative dsRNA-response present eschew common PKR-based system. These results further show how employ distinct strategies antiviral defense tolerating infections. They suggest bats, function not observed mammals. highlights mechanism originating evolve features could extreme responses such as is seen with SARS-CoV-2.

Язык: Английский

Процитировано

0
Extensive longevity and DNA virus-driven adaptation in nearctic Myotis bats DOI Creative Commons
Juan Manuel Vázquez, M. Elise Lauterbur, Saba Mottaghinia

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 11, 2024

Abstract The genus Myotis is one of the largest clades bats, and exhibits some most extreme variation in lifespans among mammals alongside unique adaptations to viral tolerance immune defense. To study evolution longevity-associated traits infectious disease, we generated near-complete genome assemblies cell lines for 8 closely related species . Using genome-wide screens positive selection, analyses structural variation, functional experiments primary lines, identify new patterns adaptation contributing longevity, cancer resistance, interactions bats. We find that bats have significant risk across demonstrate a DNA damage response cells long-lived M. lucifugus also evidence abundant viruses - but not RNA other sharp contrast with mammals, potentially role as reservoirs zoonoses. Together, our results how genomics derived from diverse taxa uncover molecular bases non-model organisms.

Язык: Английский

Процитировано

2